Papers and Patents using Spy technology Many of these publications don’t come up from a Pubmed search or citation search, so it should be useful to bring them all together and hyperlinked (now >1,200 publications). Patents may be omitted if there is a journal paper covering very similar work. Ones with particular relevance to IMMUNOLOGY, MATERIALS (polymers or mechanobiology), IMAGING , SEQUENCING or ENZYMES are marked. For an overview, please see our open-access review “Power to the Protein” from 2020. SpyBank is a database of >1,000 Tag/Catcher aa sequences to download (Excel file), from publications below. SpyBank includes includes expression organism, Tag/Catcher location (N-terminal, internal, C-terminal) and annotated linkers, as a reference for groups creating their own fusions. SpyBank itself is described in: “Insider information on successful covalent protein coupling with help from SpyBank. Keeble AH, Howarth M. Meth Enz 2019 PDF”. (See note on codon usage at Reagents page). Most recent below. Publications 601-900 here. Publications 301-600 here. Publications 1-300 here. Engineering a Fusion Protein Biomaterial Based on SpyTag/SpyCatcher Bioconjugation of Elastin and Collagen Synthetic Proteins Pedro Toledo-Garcia…Alberto Donayre-Torres. ACS Omega April 16, 2025 MATERIALS HETERODIMERIC PROTEIN PRODUCTION METHOD, DIMERIC PROTEIN, MONOMERIC PROTEIN, AND TARGET RESPONSIVE HETERODIMERIC PROTEIN SCREENING METHOD European Patent Office Application Number 23811901 Application Date 26.05.2023 Inventors MAKABE KOKI et al. 1225 Detection and isolation of viable cancer cells mediated by spytag and spycatcher using conditionally replicative adenovirus and magnetic microbeads Sadegh Goli…Kayhan Azadmanesh. Scientific Reports volume 15, Article number: 11243 (2025) MATERIALS Development of a versatile and high-loading capacity yeast surface assembly system via SpyC/SpyT conjugation Ke Xu…Guimin Zhang. Process Biochemistry 12 April 2025 Hydrogels with prestressed tensegrity structures Bin Xue…Yi Cao. Nature Communications volume 16, Article number: 3637 (2025) MATERIALS METHODS AND MOLECULAR CONSTRUCTS FOR INCORPORATING MOLECULES INTO ELECTRICAL CIRCUITS Patent WO/2025/080785 Publication Date 17.04.2025 Applicants ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY [US]/[US] RECOGNITION ANALYTIX, INC. [US]/[US] MATERIALS POLYPEPTIDES AND PEPTIDE TAGS CAPABLE OF SPONTANEOUS ISOPEPTIDE BOND FORMATION AT LOW TEMPERATURE AND LOW PH AND USES THEREOF WO/2025/074091, Publication Date 10.04.2025 Inventors: READING, Eamonn; HAMMERSCHMID, Dietmar; HOWARTH, Mark; KEEBLE, Anthony SYSTEMS OF SINGLE-CELL LABELING AND METHODS OF USING THE SAME Patent Application Number 18902306 Publication Date 03.04.2025 Inventor Amelia ESCOLANO IMMUN GENERATION OF ANTIGEN-SPECIFIC T AND B CELLS USING ENGINEERED COMMENSALS Patent WO/2025/076409 Publication Date 10.04.2025 Inventors FISCHBACH, Michael, A. et al. IMMUN EPITOPE PEPTIDE AND ANTIBODY FOR TREATING HBV INFECTION AND RELATED DISEASES Patent 18832696 Publication Date 27.03.2025 Applicants XIAMEN UNIVERSITY, YANG SHENG TANG COMPANY, LTD. IMMUN BIOENGINEERING MICROBIAL ENCAPSULIN NANO-PARTICLES AS EFFICACIOUS NANOMEDICINE FOR NUCLEIC ACIDS Patent WO/2025/068517 Publication Date 03.04.2025 Inventors HELALAT, Seyed Hossein; SUN, Yi IMMUN Destabilising Effect of Class B CpG Adjuvants on Different Proteins and Vaccine Candidates Kawkab Kanjo…Raghavan Varadarajan. Vaccines 2025, 13(4), 395 IMMUN A customized multienzyme cascade immobilization system for efficiently biosynthesizing allitol Xuanzhen Ma…Hui-Min Qin. Food Bioscience 9 April 2025, 106578 ENZ Multiphasic protein condensation governed by shape and valency Vikas Pandey…Hidetoshi Urakubo. Cell Reports 7 April 2025, 115504 MATERIALS AMINO ACID BINDING AGENTS AND THEIR USES Patent WO/2025/064836 Publication Date 27.03.2025 Applicants GLYPHIC BIOTECHNOLOGIES, INC. [US]/[US] SEQUENCING Continuous Protein Sensing Using Fast-Dissociating Antibody Fragments in Competition-Based Biosensing by Particle Motion Claire M. S. Michielsen…Menno W. J. Prins. ACS Sensors 2025 Mar Enhancement of Lycopene Biosynthesis Using Self-Assembled Multi-Enzymic Protein Cages Yulong Zhou…Bing Tian. Microorganisms 2025, 13(4), 747 ENZ Extracellular Bacterial Production of DNA Hydrogels–Toward Engineered Living Materials Philipp Gaspers…Christof M. Niemeyer. Small 2025 Mar MATERIALS A combined adjuvant and ferritin nanocage based mucosal vaccine against Streptococcus pneumoniae induces protective immune responses in a murine model Tien Duc Nguyen…Joon Haeng Rhee. Nature Communications volume 16, Article number: 2871 (2025) IMMUN VERSATILE IMMUNOGENIC MODULES AND NANOPARTICLES FOR TICK VACCINES WO/2025/057109 Publication Date 20.03.2025 Inventors CORREA, Agustin et al. IMMUN Mechanically weak and highly dynamic state of mechanosensitive titin Ig domains induced by proline isomerization Yukai Wang…Shimin Le. Nature Communications volume 16, Article number: 2771 (2025) MATERIALS Controlled Enzyme Cargo Loading in Engineered Bacterial Microcompartment Shells Nicholas M. Tefft…Michaela A. TerAvest. Biochemistry 2025 Mar MATERIALS ENZ The SpyBLI cell-free pipeline for the rapid quantification of binding kinetics from crude samples Olga Predeina…Mark R. Howarth, Pietro Sormanni. bioRxiv 2025 March Functional and structural profiling of circulation via genetically encoded modular fluorescent probes. Marta Vittani…Hajime Hirase. bioRxiv 2025 Mar IMAGING PRODUCTION AND PURIFICATION OF COVALENTLY SURFACE MODIFIED ADENO-ASSOCIATED VIRUS Patent WO/2025/054526 Publication Date 13.03.2025 REGENERON PHARMACEUTICALS, INC. [US]/[US] IMMUN NOVEL HEPATITIS C VIRUS (HCV) IMMUNOGENS AND RELATED USES Patent WO/2025/054380 Publication Date 13.03.2025 Inventors HE, Linling et al. IMMUN SYSTEMS AND METHODS FOR GENERATING MULTIVALENT MOSAIC HLA NANOPARTICLES FOR PERSONALIZED IMMUNOTHERAPY APPLICATIONS Patent WO/2025/049742 Publication Date 06.03.2025 SGOURAKIS, Nikolas et al. IMMUN 1200 A BIVALENT POLYPEPTIDE SCAFFOLD AND USES THEREOF Patent WO/2025/046229 Publication Date 06.03.2025 HOWARTH, Mark and DRISCOLL, Claudia IMMUN OMEGA-guided DNA polymerases enable random mutagenesis in a tunable window Li-Hua Liu…Ao Jiang. Trends Biotech 2025 Mar ENZ INJECTABLE HYDROGEL FOR SUSTAINED CO-DELIVERY OF AN ANTIGEN AND AN ADJUVANT, AND USES THEREOF Patent United States of America Application Number 18826093 Szu-Wen Wang et al. MATERIALS IMMUN ALPHA-HELICAL PROTEIN NANOFIBRILS Patent WO/2025/046122 Publication Date 06.03.2025 REMAUT, Han et al. MATERIALS CATENATED VAR2CSA RECOMBINANT PROTEIN, PREPARATION METHOD THEREFOR, AND USE THEREOF Patent USA Application Number 18727302 Application Date 09.06.2022 CAS LAMVAC (GUANGZHOU) BIOMEDICAL TECHNOLOGY IMMUN Surface display of eugenol oxidase and dioxygenase complex as a sustainable biocatalyst for efficient bioconversion of lignin-derived 4-n-propylguaiacol to vanillin Yongqing Tian…Jing Wo. Microbial Cell Factories volume 24, Article number: 54 (2025) ENZ Reversible peptide tagging system to regulate enzyme activity Yao Chen…Ling Jiang. Cell Reports Physical Sciences 4 March 2025, 102462 ENZ Preparation of multimeric peptide-MHC with SpyCatcher modules for antigen-specific T cell detection Xiangyao Wang…Si Liu. Sensors and Actuators B 430, 1 May 2025, 137342 IMMUN Construction of a Cofactor Self-Sufficient Enzyme Cascade System Coupled with Microenvironmental Engineering for Efficient Biosynthesis of Tetrahydrofolate and Its Derivative of L-5-Methyltetrahydrofolate Ziting Yan…Kequan Chen. Catalysts 2025, 15(3), 235 ENZ Intermolecular Misfolding Captured in Parallelly Organized Titin Jiacheng Zuo…Hongbin Li. Journal of the American Chemical Society Vol 147/Issue 6 February 2, 2025 MATERIALS Simplified MS2 phage-like particle production including a novel maturation protein internal fusion affinity tag Enos C. Kline…Barry R. Lutz. Biotechnology & Biotechnological Equipment Volume 39, 2025 - Issue 1 MATERIALS Development and Efficacy Evaluation of a Novel Nanoparticle-Based Hemagglutination Inhibition Assay for Serological Studies of Porcine Epidemic Diarrhea Virus Fengyan Liang…Yifei Lang. Vet. Sci. 2025, 12(2), 101 IMMUN Tetravalent Virus-like Particles Engineered To Display Envelope Domain IIIs of Four Dengue Serotypes in Silkworm as Vaccine Candidates Krishna Raja…Enoch Y. Park. Biomacromolecules February 3, 2025 IMMUN In vivo targeted gene delivery using Adenovirus-antibody molecular glue conjugates Paul J. Rice-Boucher…Zhi Hong Lu. bioRxiv 2025 Feb IMMUN FETCH enables fluorescent labeling of membrane proteins in vivo with spatiotemporal control in Drosophila Kevin D. Rostam…Richard S. Mann. bioRxiv 2025 Feb IMAGING The Power of Three: Dynactin associates with three dyneins under load for greater force production Lu Rao…Arne Gennerich. bioRxiv 2025 Jan ENZ Design and characterization of HIV-1 vaccine candidates to elicit antibodies targeting multiple epitopes Harry B. Gristick…Pamela J. Bjorkman. bioRxiv 2025 Jan IMMUN LIFETIME ALPHA-HEMOLYSIN NANOPORES US Patent Application Number 18798279 Publication Date 30.01.2025 Applicants Roche Sequencing Solutions, Inc. SEQUENCING DESIGN OF IMMUNOGENS THAT PREFERENTIALLY INTERACT WITH B CELL RECEPTORS CONTAINING COMPLEMENTARY DETERMINING REGION LOOPS OF SPECIFIC COMPOSITION Patent WO/2025/029745 Publication Date 06.02.2025 Inventors AZOITEI, Mihai IMMUN Engineering high-activity crosslinked enzyme aggregates via SpyCatcher/SpyTag-mediated self-assembly Xueliang Ma…Lili Jia. International Journal of Biological Macromolecules 295 (2025) 139591 MATERIALS ENZ Resolving native GABAA receptor structures from the human brain Jia Zhou…Ryan E. Hibbs. Nature 2025 Jan DIRECTLY FUNCTIONALIZED ELECTROCHMICAL TRANSISTEORS, AND CONVECTION DRIVEN ULTRA-RAPID DETECTION OF BIOMARKERS USING TRANSISTORS Patent Application Number 18711844 Publication Date 23.01.2025 Inventors Sahika Inal et al. MATERIALS AFFINITY PURIFICATION, PROXIMITY-BASED SORTASE LIGATION, AND DETECTION OF PROTEINS WITH PRECURSOR PEPTIDES AND B1 PROTEINS FROM LASSO PEPTIDE BIOSYNTHESIS SYSTEMS Patent Application Number 18716935 Publication Date 23.01.2025 Applicants UNIVERSITY OF PENNSYLVANIA AlphaThera LLC Inventors Andrew TSOURKAS et al. GENE THERAPY DELIVERY COMPOSITIONS AND METHODS OF USE THEREOF Patent Application Number 18775975 Publication Date 23.01.2025 Inventors Logan Collins et al. In Situ Bioconjugation of Synthetic Peptides onto Universal Chimeric Antigen Receptor T Cells for Targeted Cancer Immunotherapies Ian I. Cardle…Drew L. Sellers. ACS Nano 2025 Jan IMMUN 1175 Programmable Scaffold-Mediated Assembly Regulation Tool for Dynamic Control of a Multienzyme Biocatalyst Mengkai Hu…Zhiming Rao. ACS Catalysis 2025 Jan ENZ Designed mosaic nanoparticles enhance cross-reactive immune responses in mice Eric Wang…Arup K. Chakraborty. Cell 2025 Jan IMMUN Programing Green Light-Responsive Hydrogels: from Photo-Weakening to Photo-unresponsiveness and Photo-Strengthening Saskia Frank, Seraphine Wegner. Chemistry 14 January 2025 MATERIALS Designing a microbial factory suited for plant chloroplast-derived enzymes to efficiently and green synthesize natural products: capsanthin and capsorubin as examples Huibin Chen…Zhen Liu. Metabolic Engineering 16 January 2025 ENZ Ferritin nanoparticles significantly enhance the immune response to the African swine fever virus p34 protein Rong Zhang…Haixue Zheng. International Journal of Pharmaceutics 16 January 2025 IMMUN SPATIAL PROXIMITY ASSAY Patent WO/2025/012402 Publication Date 16.01.2025 Inventors HINNAH, Konstantin, WILHELM, Jonas, JOHNSSON, Kai Optimising CAR-T cell sensitivity by engineering matched extracellular sizes between CAR/antigen and CD2/CD58 adhesion complexes Jake Burton…Omer Dushek. bioRxiv 2025 Jan IMMUN Nanoparticle Vaccine Triggers Interferon-Gamma Production and Confers Protective Immunity against Porcine Reproductive and Respiratory Syndrome Virus Yangyang Sun…Ping Jiang. ACS Nano 2025 Jan IMMUN MagIC-Cryo-EM: Structural determination on magnetic beads for scarce macromolecules in heterogeneous samples Yasuhiro Arimura…Hironori Funabiki. bioRxiv 2025 Jan MATERIALS Hidden route of protein damage through oxygen-confined photooxidation Seoyoon Kim…Duyoung Min. Nature Communications volume 15, Article number: 10873 (2024). MATERIALS Development of a Tick-Borne Encephalitis Virus Nanoparticle Vaccine Utilizing Envelope Dimer Bingjie Wang, Haiyan Zhao. bioRxiv 2024 Nov IMMUN Adaptive multi-epitope targeting and avidity-enhanced nanobody platform for ultrapotent, durable antiviral therapy Yufei Xiang…Yi Shi. Cell 2024 Nov In situ forming and self-crosslinkable protein hydrogels for localized cancer therapy and topical wound healing Soomin Eom…Sebyung Kang. J Cont Rel 2025 Feb MATERIALS CONJUGATED MOLECULES Patent WO/2024/256843 Publication Date 19.12.2024 Applicants VALINK THERAPEUTICS LTD [GB]/[GB] METHODS FOR PURIFYING FILLED ADENO-ASSOCIATED VIRUS CAPSIDS Patent WO/2024/263705 Publication Date 26.12.2024 REGENERON PHARMACEUTICALS, INC. [US]/[US] Preparation and immunogenicity evaluation of ferritin nanoparticles conjugated with African swine fever virus p30 protein Yue Zhang…Haixue Zheng. Sheng Wu Gong Cheng Xue Bao 2024 Dec 25;40(12):4509-4520. IMMUN NOVEL BINDERS TARGETING THE MULTI-DRUG RESISTANT PATHOGEN ACINETOBACTER BAUMANNII Patent WO/2024/261344 Publication Date 26.12.2024 Inventors VAN DER HENST, Charles et al. METHOD FOR SCREENING A MOLECULE FOR BINDING TO A PROTEIN OF INTEREST Patent WO/2024/256374 Publication Date 19.12.2024 Inventors GILLINGHAM, Dennis Development of a Versatile Cancer Vaccine Format Targeting Antigen-Presenting Cells Using Proximity-Based Sortase A-Mediated Ligation of T‑Cell Epitopes Aru Z. Wang…Joke M. M. den Haan. Bioconj Chem 2024 Dec IMMUN Hierarchical design of pseudosymmetric protein nanocages Quinton M. Dowling…Neil P. King. Nature 2024 Dec MATERIALS SpyFixer enables efficient site-specific immobilization for protein-protein interaction analysis and antibody purification Ya Xiang…Xiaofeng Yang. International Journal of Biological Macromolecules 287, January 2025, 138548 MATERIALS Clickable, Thermally Responsive Hydrogels Enabled by Recombinant Spider Silk Protein and Spy Chemistry for Sustained Neurotrophin Delivery Yue Li…Fei Sun. Adv Mat 2024 Dec MATERIALS Modular functionalization of cellular nanodiscs enables targeted delivery of chemotherapeutics into tumors Ilkoo Noh…Liangfang Zhang. J Cont Release 2025 Feb MATERIALS Delivery of dendritic cells targeting 3M2e-HA2 nanoparticles with a CpG adjuvant via lysosomal escape of Salmonella enhances protection against H9N2 avian influenza virus Futing Jia…Yanlong Jiang. Poultry Science 2025 IMMUN Development of a self-assembled dual-enzyme co-display platform on the surface of the natural “chitosan beads” of yeast spores Jiawen Bai…Zijie Li. International Journal of Biological Macromolecules 286, January 2025, 138308 MATERIALS ENZ 1150 EPITOPE PEPTIDE AND ANTIBODY FOR TREATING HBV INFECTION AND RELATED DISEASES European Patent Office Application Number 23746324 Publication Date 04.12.2024 Inventors LUO WENXIN et al. IMMUN Construction of Chitinase Complexes Using Self-Assembly Systems for Efficient Hydrolysis of Chitin Zhewei Shen…Chenggang Xu. ACS Synbio 2024 Nov ENZ Modular Tunable α-Helical Peptide Hydrogels for Neuronal Cells D. Arne Scott…Derek N. Woolfson. Adv Funct Mat 2024 Nov MATERIALS Remodelling of Rea1 linker domain drives the removal of assembly factors from pre-ribosomal particles Johan Busselez…Helgo Schmidt. Nature Communications volume 15, Article number: 10309 (2024) MagIC-Cryo-EM: Structural determination on magnetic beads for scarce macromolecules in heterogeneous samples Yasuhiro Arimura…Hironori Funabiki. eLife 2024 Nov MATERIALS MULTIPLEXED PROFILING OF RNA AND DNA MODIFICATIONS Patent United States of America Publication Date 21.11.2024 ALIDA BIOSCIENCES INC. ANTI-VIRUS MOIETY IMMOBILISED IN A MATRIX Patent United States of America Application Number 18690098 Publication Date 14.11.2024 SAMATVA RESEARCH CORPORATION MATERIALS N-TERMINAL MODIFIER AGENTS AND BINDERS FOR TREATING AND ANALYZING PEPTIDES Patent United States of America Publication Date 14.11.2024 Applicants Encodia, Inc. SEQUENCING Towards Precision Biocatalysis - Leveraging Inline NMR for Autonomous Experimentation in Flow Reactors Felix Ott…Christof M. Niemeyer. Chemistry-Methods 2024 Nov MATERIALS ENZ Modular Display of Plasmodium yoelii Circumsporozoite Surface Protein and Merozoite Surface Protein-1 on Norovirus-like Particles Jirayu Boonyakida…Enoch Y. Park. Bioconj Chem 2024 Nov IMMUN Tuning architectural organization of eukaryotic P450 system to boost bioproduction in Escherichia coli Yikui Li…Ren Wang. Nature Communications volume 15, Article number: 10009 (2024) ENZ The Photocleavable Protein PhoCl-Based Dynamic Hydrogels Jingqi Lei…Hongbin Li. ACS Biomat Sci 2024 Nov MATERIALS Mechanically Tunable, Compostable, Healable and Scalable Engineered Living Materials Avinash Manjula-Basavanna…Neel S. Joshi. Nature Communications volume 15, Article number: 9179 (2024) MATERIALS SPYCATCHER ANALOGS AND USES THEREOF Patent WO/2024/231728 Publication Date 14.11.2024 BIO-RAD ABD SEROTEC GMBH [DE]/[DE] PLASMONIC NANOANTENNA AND USE OF SAME IN SINGLE MOLECULE SEQUENCING Patent WO/2024/229524 Publication Date 14.11.2024 NEWSOUTH INNOVATIONS PTY LIMITED [AU]/[AU] MATERIALS SEQUENCING METHOD FOR DETECTING INTERACTIONS Patent WO/2024/231680 Publication Date 14.11.2024 Inventors DUSHEK, Omer et al. VIRAL ANTIGEN DISPLAY COMPOSITIONS AND METHODS Patent United States of America Publication Date 07.11.2024 Alon M. Herschhorn IMMUN Engineering the Mechanical Stability of a Therapeutic Complex between Affibody and Programmed Death-Ligand 1 by Anchor Point Selection Byeongseon Yang…Michael A. Nash. ACS Nano 2024 Nov IMMUN MATERIALS A Versatile Method to Create Antibody/Split-Enzyme Complexes and Its Application to a Rapid, Homogeneous, and Universal Electrochemical Immunosensing System Yuka Tobita…Ryutaro Asano. Adv Sensor Res 2024 Oct ENZ VP8 Mosaic Nanoparticles Elicit Cross-Neutralizing Immune Responses and Provide Protection Against Heterotypic Rotavirus Challenge in Mice Feibo Song…Ningshao Xia. ACS Nano 2024 Nov IMMUN Rationally designed multimeric nanovaccines using icosahedral DNA origami for display of SARS-CoV-2 receptor binding domain Qingqing Feng…Xiao Zhao. Nat Comm 2024 Nov IMMUN MATERIALS Development of a Versatile Cancer Vaccine Format Targeting Antigen-Presenting Cells Using Proximity- Based Sortase A-Mediated Ligation of T-Cell Epitopes Aru Z. Wang…Joke M. M. den Haan. Bioconj Chem 2024 Nov IMMUN ANTI-HUMAN M-CADHERIN (CDH15) ANTIBODIES, CONJUGATES, AND USES THEREOF FOR DELIVERY OF GENETIC PAYLOADS TO MUSCLE CELLS Patent WO/2024/229105 Publication Date 07.11.2024 REGENERON PHARMACEUTICALS, INC. [US]/[US] Design of a Genetically Programmable and Customizable Protein Scaffolding System for the Hierarchical Assembly of Robust, Functional Macroscale Materials Ruijie Zhang…Claudia Schmidt-Dannert. ACS Syn Biol 2024 Oct MATERIALS Engineered Outer Membrane Vesicles as Nanosized Immune Cell Engagers for Enhanced Solid Tumor Immunotherapy Jianan Sun…Xiaobao Bi. ACS Nano 2024 Oct IMMUN 1125 Light-Based Juxtacrine Signaling Between Synthetic Cells Hossein Moghimianavval, Kyle J. Loi, Sung-Won Hwang, Yashar Bashirzadeh, Allen P. Liu. Small Science 2024 Oct MATERIALS CD11A TAGE COMPOSITIONS AND USES THEREOF Patent WO/2024/226822 Publication Date 31.10.2024 SPOTLIGHT THERAPEUTICS [US]/[US] In-Situ Structure and Topography of AMPA Receptor Scaffolding Complexes Visualized by CryoET Richard G. Held…Axel T. Brunger. bioRxiv 2024 Oct MATERIALS CAPSID ENGINEERED ADENO-ASSOCIATED VIRUSES Patent WO/2024/220714 Publication Date 24.10.2024 Inventors SCHMIDT, Daniel et al. Magnetic protein aggregates generated by supramolecular assembly of ferritin cages - a modular strategy for the immobilization of enzymes Gizem Olcucu…Ulrich Krauss. Front Bioeng 2024 Oct MATERIALS The liquid lectin array detects compositional glycocalyx differences using multivalent DNA-encoded lectins on phage Guilherme M. Lima…Ratmir Derda. Cell Chem Biol 2024 Oct MATERIALS Controlled enzyme cargo loading in engineered bacterial microcompartment shells Nicholas M Tefft…Michaela A TerAvest. bioRxiv 2024 Oct MATERIALS ENZ MODULAR RE-TARGETING OF RHABDOVIRAL (G) GLYCOPROTEINS FROM THEIR NATURAL RECEPTORS Patent WO/2024/216223 Publication Date 17.10.2024 VYRIAD [US]/[US] IMMUN Developing Porous Protein Cage Nanoparticles as Cargo-Loadable and Ligand-Displayable Modular Delivery Nanoplatforms Soomin Eom…Sebyung Kang. ACS Applied Materials & Interfaces 2024 Oct MATERIALS Transient CAR T cells with specificity to oncofetal glycosaminoglycans in solid tumors Nastaran Khazamipour…Mads Daugaard. EMBO Mol Med 2024 Oct IMMUN Experimental VLP vaccine displaying a furin antigen elicits production of autoantibodies and is well tolerated in mice Vili Lampinen…Vesa Hytönen. Nanoscale Advances 2024 IMMUN Coupling of Spectrin Repeat Modules for the Assembly of Nanorods and Presentation of Protein Domains Klemen Mezgec…Roman Jerala. ACS Nano 2024 MATERIALS Folds from fold: Exploring topological isoforms of a single-domain protein Zhiyu Qu…Wen-Bin Zhang. PNAS 2024 121 (43) e2407355121 MATERIALS Construction of bispecific antibodies by specific pairing between the heavy chain and the light chain using removable SpyCatcher/SnoopCatcher units Jyunna Yoshida…Koki Makabe. Journal of Biological Engineering volume 18, Article number: 57 (2024) IMMUN Loop Grafting Improves Neuraminidase Protein Vaccine Production Pramila Rijal, Leiyan Wei, Guido C Paesen, David I Stuart, Mark R Howarth, Kuan-Ying A Huang, Thomas A Bowden, Alain RM Townsend. bioRxiv 2024 Oct IMMUN Plug and play virus-like particles for the generation of anti-toxin antibodies Rebecca J. Edge…Stuart Ainsworth. Toxicon X 2024 September IMMUN Development of a Two-Component Nanoparticle Vaccine Displaying an HIV-1 Envelope Glycoprotein that Elicits Tier 2 Neutralising Antibodies. Malebo, K…Chapman, R. Vaccines 2024 Oct IMMUN Development and Characterization of A Novel SpyTagged Modular Nanobody as A Detection Platform for CD22-Positive Cells Amirhosein Maali…Zahra Sharifzadeh. Cell J 2024 Oct 7;26(8):487-495. IMMUN A Modular Bacteriophage T4 Nanoparticle Platform Enables Rapid Design of Completely Protective Multivalent Mucosal Dual Vaccine Against COVID-19 and Flu Jingen Zhu…Venigalla B. Rao. bioRxiv 2024 Oct IMMUN Modular Nano-Antigen Display Platform for Pigs Induces Potent Immune Responses Yilan Chen…Gaiping Zhang. ACS Nano 2024 Oct IMMUN Modulation of SpyCatcher Ligation Kinetics by SpyTag Thioamide Substitution Kristen E. Fiore…E. James Petersson. bioRxiv 2024 Oct A Versatile Magnetic Nanoplatform for Plug-and-Play Functionalization: Genetically Programmable Cargo Loading to Bacterial Magnetosomes by SpyCatcher “Click Biology” Frank Mickoleit…Dirk Schüler. ACS Nano 2024 Oct MATERIALS Preparation of Bio-Orthogonal Self-Cross-Linked Arabinose Isomerase for Enzymatic Synthesis of ʟ-Ribulose Houtian Luo…Li Qiao. Chemistry 03 October 2024 ENZ Single-molecule force spectroscopy reveals intra- and intermolecular interactions of Caenorhabditis elegans HMP-1 during mechanotransduction Shimin Le…Jie Yan. PNAS 2024 Jul MATERIALS A Spy Chemistry-Based Method for Purification of Proteins with Authentic N-Termini by Xiaofeng Yang…Zhanglin Lin. Catalysts 2024, 14(9), 651 1100 Targeted Delivery of BCL-2 siRNA Using Modified Ferritin Nanocarriers for Antitumor Therapy Li-Miao Qin…Yuqing Wu. ACS Applied Nano Materials September 26, 2024 IMMUN Proximity-Unlocked Luminescence by Sequential Enzymatic Reactions from Antibody and Antibody/Aptamer (PULSERAA): A Platform for Detection and Visualization of Virus-Containing Spots Daimei Miura…Ryutaro Asano. Advanced Science 2024 Sept METHODS FOR IN VIVO EDITING OF B CELLS Patent WO/2024/196669 Publication Date 26.09.2024 WALKING FISH THERAPEUTICS, INC. [US]/[US], WASHINGTON UNIVERSITY [US]/[US] IMMUN ARTIFICIAL COMPLEX FOR TETHERING CHROMATIDS OR CHROMOSOMES Patent WO/2024/194344 Publication Date 26.09.2024 Inventors SCHUH, Melina et al. Repurposing Copper(II)/THPTA as A Bioorthogonal Catalyst for Thiazolidine Bond Cleavage Chengyun Ma…Xiaobao Bi. Advanced Science19 September 2024 Ferritin nanoparticle-based Nipah virus glycoprotein vaccines elicit potent protective immune responses in mice and hamsters Shaohong Chen…Sandra Chiu. Virologica Sinica 16 September 2024 IMMUN Dynamic allostery drives autocrine and paracrine TGF-β signaling Mingliang Jin…Yifan Cheng. Cell 2024 Sept A Self-Assembling γPFD-SpyCatcher Hydrogel Scaffold for the Coimmobilization of SpyTag-Enzymes to Facilitate the Catalysis of Regulated Enzymes Bei-Ping…Fu-Xing Niu. Journal of Agricultural and Food Chemistry Vol 72/Issue 36 August 28, 2024. MATERIALS Ligand requirements for immunoreceptor triggering Michael I. Barton…Philip Anton van der Merwe. Communications Biology volume 7, Article number: 1138 (2024) IMMUN ANTIBODY-GUIDED PCSK9-MIMICKING IMMUNOGENS LACKING 9-RESIDUE SEQUENCE OVERLAP WITH HUMAN PROTEINS Patent 18270436 Application Date 31.12.2021 Inventors Peter D. Kwong et al. IMMUN COMPOSITIONS AND METHODS FOR PRODUCING GLYCOCONJUGATE POLYPEPTIDES HAVING ISOPEPTIDE BONDS WITH A SECOND POLYPEPTIDE PARTNER AND USES THEREOF WO/2024/182291 Publication Date 06.09.2024 Applicants VAXNEWMO LLC [US]/[US] IMMUN HIGH-THROUGHPUT SEROTYPING AND ANTIBODY PROFILING ASSAYS Patent Application Number 18658862 Application Date 08.05.2024 Applicants Encodia, Inc. IMMUN SEQUENCING SEQUENTIAL ENCODING METHODS AND RELATED KITS Patent Application Number 18586030 Application Date 23.02.2024 Applicants Encodia, Inc. SEQUENCING Genetically surface-modified Escherichia coli outer membrane vesicles targeting MUC1 antigen in cancer cells Sedthawut Laotee, Wanatchaporn Arunmanee. Biotechnology Reports Volume 44, December 2024, e00854 IMMUN An Antibiotic-Degrading Engineered Biofilm Platform to Combat Environmental Antibiotic Resistance Gökçe Özkul…Şafak Şeker. ACS Biomaterials Science & Engineering September 3, 2024 MATERIALS DNA-Directed Activation of Photocatalytic Labeling at Cell–Cell Contact Sites Xu Zhou…Jeffrey D. Martell. ACS Chemical Biology September 3, 2024 Leveraging Immunofocusing and Virus-like Particle Display to Enhance Antibody Responses to the Malaria Blood- Stage Invasion Complex Antigen PfCyRPA by Kasper H. Björnsson…Lea Barfod. Vaccines 2024, 12(8), 859 IMMUN CD13-targeting and TRAIL-displaying protein nanoparticles effectively induce apoptotic cell death of acute myeloid leukemia, prolonging survival in mouse models Heejin Jun…Sebyung Kang. Nano Today Volume 59, December 2024, 102474 Tumor-agnostic cancer therapy using antibodies targeting oncofetal chondroitin sulfate Elena Ethel…Ali Salanti. Nature Communications volume 15, Article number: 7553 (2024) IMMUN Directional co-immobilization of artificial multimeric-enzyme complexes as a robust biocatalyst for biosynthesis curcumin glucosides and regeneration of UDP-glucose Mohamed Yassin Ali…Daochen Zhu. International Journal of Biological Macromolecules Volume 278, Part 4, October 2024, 135035 ENZ Stepping dynamics of dynein characterized by MINFLUX Joseph Slivka…Ahmet Yildiz. bioRxiv 2024 ENZ IMAGING Autoinhibition of cMyBP-C by its middle domains A.C. Greenman…S.P. Harris. bioRxiv 2024 MATERIALS Design of a Soluble Multivalent Notch Agonist Rubul Mout…George Q Daley. bioRxiv 2024 The role of protein shape in multiphasic separation within condensates Vikas Pandey…Hidetoshi Urakubo. bioRxiv 2024 MATERIALS Phollow: Visualizing Gut Bacteriophage Transmission within Microbial Communities and Living Animals Lizett Ortiz de Ora…Travis J Wiles. Nature Microbiology (2025) IMAGING 1075 Nonlinear compliance of NompC gating spring and its implication in mechanotransduction Yukun Wang…Yongli Zhang. bioRxiv 2024 MATERIALS SubtiToolKit - a bioengineering kit for Bacillus subtilis and Gram-positive bacteria Joaquin Caro-Astorga…Tom Ellis. bioRxiv 2024 Interplay of kinetochores and catalysts drives rapid assembly of the mitotic checkpoint complex Suruchi Sethi…Andrea Musacchio. bioRxiv 2024 Conditional CAR T cells with specificity to oncofetal glycosaminoglycans in solid tumors Nastaran Khazamipour…Mads Daugaard. bioRxiv 2024 IMMUN Engineering the Mechanical Stability of a Therapeutic Affibody/PD-L1 Complex by Anchor Point Selection Byeongseon Yang…Michael A. Nash. bioRxiv 2024 MATERIALS CHIMERIC PROTEINS AND THEIR USE AS AN ANTIGEN DETECTING SYSTEM Patent WO/2024/175675 cPublication Date 29.08.2024 Inventors FERNÁNDEZ HERRERO, Luis Ángel et al. IMMUN Endogenous capsid-forming protein ARC for self-assembling nanoparticle vaccines Yu Li…Junjie Xu. Journal of Nanobiotechnology volume 22, Article number: 513 (2024) IMMUN Mosaic sarbecovirus nanoparticles elicit cross-reactive responses in pre-vaccinated animals Alexander A. Cohen…Pamela J. Bjorkman. Cell 2024 Aug IMMUN Directional co-immobilization of artificial multimeric-enzyme complexes as a robust biocatalyst for biosynthesis curcumin glucosides and regeneration of UDP-glucose Mohamed Yassin Ali…Daochen Zhu. International Journal of Biological Macromolecules 278, Part 4, October 2024, 135035 ENZ A SARS-CoV-2 peptide antigen purified from bacteria and displayed in a high-density repetitive manner on a virus-like particle could generate anti-SARS-CoV- 2 neutralizing antibodies unlike free peptide Feroza Begum…Upasana Ray. BBRC Aug 2024 IMMUN CTC-derived pancreatic cancer models serve as research tools and are suitable for precision medicine approaches Jiajia Tang…Christopher Heeschen. Cell Reports Medicine August 19, 2024 A Click-Type Enzymatic Method for Antigen-Adjuvant Conjugation Yange Sun…Li Zhu. Small Methods 23 August 2024 IMMUN TREATMENT OF MUSCLE RELATED DISORDERS WITH ANTI-HUMAN CACNG1 ANTIBODIES Patent WO/2024/173248 Publication Date 22.08.2024 Applicants REGENERON PHARMACEUTICALS, INC. [US]/[US] POLYPEPTIDE FRAGMENTS, IMMUNOGENIC COMPOSITION AGAINST SARS-CoV-2, AND IMPLEMENTATIONS THEREOF US Patent 18004065 Publication Date 08.08.2024 MYNVAX PRIVATE LIMITED IMMUN COMPOSITIONS AND METHODS FOR CONTROLLED MRNA TRANSLATION AND STABILITY US patent 18392928 Publication Date 08.08.2024 Applicants TRUSTEES OF BOSTON UNIVERSITY Site-specific tethering nanobodies on recombinant adeno-associated virus vectors for retargeted gene therapy Yuanjie Zhang…Dezhong Ji. Acta Biomaterialia 2024 Aug Influenza virus strains expressing SARS-CoV-2 receptor binding domain protein confer immunity in K18-hACE2 mice Nathaniel A. Rader…F. Heath Damron. Vaccine Volume 20, October 2024, 100543 IMMUN Reinforcing thermostability and pH robustness of exo-inulinase facilitated by ReverseTag/ReverseCatcher tagging system Xixi Xie…Ling Jiang. International Journal of Biological Macromolecules 9 August 2024, 134502 ENZ Virus-like particles displaying the mature C-terminal domain of filamentous hemagglutinin are immunogenic and protective against Bordetella pertussis respiratory infection in mice Gage M Pyles…Mariette Barbier. Infect Immun 2024 Jul 18:e0027024. IMMUN Controlled labelling of tracer antibodies for time-resolved fluorescence-based immunoassays Anastasiia Kushnarova-Vakal…Urpo Lamminmäki. Scientific Reports volume 14, Article number: 18113 (2024) IMMUN EPITOPE-SCAFFOLD IMMUNOGENS FOR PANCORONAVIRUS VACCINES Patent WO/2024/155907 Publication Date 25.07.2024 Inventors AZOITEI, Mihai et al. IMMUN VACCINE Patent WO/2024/157026 Publication Date 02.08.2024 Inventors DRAPER, Simon J et al. IMMUN Probing the Topological Effects on Stability Enhancement and Therapeutic Performance of Protein Bioconjugates: Tadpole, Macrocycle versus Figure-of-Eight Yajie Liu…Wen-Bin Zhang. Adv Health Mat 2024 Aug MATERIALS Evaluation of SARS-CoV-2-Specific IgY Antibodies: Production, Reactivity, and Neutralizing Capability against Virus Variants Andrea Aebischer…Christian Grund. Int. J. Mol. Sci. 2024, 25(14), 7976 IMMUN Sustainable Spinning of Artificial Spider Silk Fibers with Excellent Toughness and Inherent Potential for Functionalization Ruxia Fan…A. Sesilja Aranko. Adv Funct Mat 26 July 2024 MATERIALS 1050 Enhancing the stress resistance of nitrile hydratase from Rhodococcus ruber via SpyTag/SpyCatcher-mediated α- and β- subunits ligation Miaomiao Wang…Huimin Yu. Molecular Biology Reports. Volume 51, article number 817, (2024) ENZ Engineering a Self-Assembled Protein Cage for Targeted Dual Functionalization Xiao Ma…Chuang Xue. Nano Lett. 2024 July IMMUN MATERIALS VCP/p97-associated proteins are binders and debranching enzymes of K48–K63- branched ubiquitin chains Sven M. Lange…Yogesh Kulathu. Nature Structural & Molecular Biology (2024) ENZ Orchestrated Codelivery of Peptide Antigen and Adjuvant to Antigen-Presenting Cells by Using an Engineered Chimeric Peptide Enhances Antitumor T-Cell Immunity Haifeng Pan…Ningshao Xia. Cancer Immunol Res (2024) 12 (7): 905–920 IMMUN Induction of neutralising antibodies against conserved rhinovirus capsid protein VP4 depends on presenting VP4 in a virus-like conformation James T. Kelly…Tobias J. Tuthill. bioRxiv 2024 Jul IMMUN Genetically Decorating Microbial Surface Curli Fiber with Biocatalytic Properties Qingxia Wang…Jinyong Yan. ACS Sustainable Chem. Eng. 2024, Jul MATERIALS ENZ COMPOSITIONS AND METHODS FOR CONTROLLED MRNA TRANSLATION AND STABILITY Patent WO/2024/137990 Publication Date 27.06.2024 Inventors MARZILLI, Alexander Michael, NGO, John Tuan Genetically Programmed Temperature-Responsive Barnacle-Derived Protein with an Enhanced Adhesion Ability Yuanxing Shi…Jinyong Yan. ACS Appl. Bio Mater. 2024 MATERIALS Engineering Mesoscale T Cell Receptor Clustering by Plug-and-Play Nanotools M Florencia Sánchez…Robert Tampé. Adv Mater 2024 Jun 25:e2310407. IMMUN PARTICLE DELIVERY SYSTEMS Patent WO/2024/129976 Publication Date 20.06.2024 Applicants SCRIBE THERAPEUTICS INC. [US]/[US] ANTIGEN-BINDING MOLECULES THAT BIND TO AAV PARTICLES AND USES WO/2024/130175 Publication Date 20.06.2024 Applicants REGENERON PHARMACEUTICALS, INC. [US]/[US] IMMUN Oriented immobilization of nanobodies using SpyCatcher/SpyTag significantly enhances the capacity of affinity chromatography Yuxiang Zhang…Yongdong Liu. J Chromatography A 2024 Jun Development of the IMP biosensor for rapid and stable analysis of IMP concentrations in fermentation broth Shibo Jiang…Suiping Zheng. Biotech J 11 June 2024 ENZ BROADLY NEUTRALIZING ANTIBODY-TEMPLATED DIVALENT IMMUNOGEN VACCINES WO/2024/123708 Publication Date 13.06.2024 UNIVERSITY OF HAWAII [US]/[US] Inventors MACPHERSON, Iain et al. IMMUN AN ARTIFICIAL PROTEIN-CAGE COMPRISING ENCAPSULATED THEREIN A GUEST CARGO United States of America Application Number 18547242 Publication Date 06.06.2024 Applicants UNIWERSYTET JAGIELLONSKI Inventors Jonathan HEDDLE et al. MATERIALS COMPOSITIONS AND METHODS FOR FIBROBLAST GROWTH FACTOR RECEPTOR 3-MEDIATED DELIVERY TO ASTROCYTES United States of America Application Number 18509030 Publication Date 30.05.2024 Regeneron Pharmaceuticals, Inc. Dual role of the peptide- loading complex as proofreader and limiter of MHC- I presentation Jamina Brunnberga…Robert Tampé. PNAS 2024 IMMUN Cu(II)/THPTA-Mediated Thiazolidine Deprotection for Living Phages and Cell Surfaces Labeling Chengyun Ma…Xiaobao Bi. ChemRxiv 07 June 2024 MATERIALS Development of an improved blood-stage malaria vaccine targeting the essential RH5-CyRPA-RIPR invasion complex Barnabas G. Williams…Simon J. Draper. Nature Communications volume 15, Article number: 4857 (2024) IMMUN AlbuCatcher for Long-Acting Therapeutics Ji Hyun Rho…Inchan Kwon. ACS Omega 2024, 9, 21, 22990–23000 Nanoparticle display of neuraminidase elicits enhanced antibody responses and protection against influenza A virus challenge M N Pascha…C A M de Haan. NPJ Vaccines. 2024 May 31;9(1):97. IMMUN Single immunization of non-adjuvanted recombinant TTFC-mi3 nanoparticle vaccine elicited a rapid and potent protective immunity against tetanus Qinggang He…Yi Zhang. Vaccine 2024 May IMMUN Microgels with Immobilized Glycosyltransferases for Enzymatic Glycan Synthesis Isabel Katja Sommerfeld…Andrij Pich. Biomacromolecules 2024 May MATERIALS ENZ Enhancing the Stress Resistance of Nitrile Hydratase from Rhodococcus ruber via SpyTag/SpyCatcher-mediated α- and β- subunits ligation Miaomiao Wang, Huimin Yu. Research Square 2024 May ENZ Design of a genetically programmable and customizable protein scaffolding system for the hierarchical assembly of robust, functional macroscale materials Ruijie Zhang…Claudia Schmidt- Dannert. bioRxiv 2024 May MATERIALS 1025 Mosaic and cocktail capsid-virus-like particle vaccines for induction of antibodies against the EPCR-binding CIDRα1 domain of PfEMP1 Ilary Riedmiller…Louise Turner. bioRxiv 2024 April IMMUN Genetically modifying the protein matrix of macroscopic living materials to control their structure and rheological properties Esther M. Jimenez…Caroline M. Ajo-Franklin. bioRxiv 2024 May MATERIALS Development and Characterization of Recombinant ADP-Ribose Binding Reagents that Allow Simultaneous Detection of Mono and Poly ADP-Ribose Shu-Ping Chiu…W. Lee Kraus. bioRxiv 2024 May Preserving ester-linked modifications reveals glutamate and aspartate mono-ADP-ribosylation by PARP1 and its reversal by PARG Edoardo José Longarini & Ivan Matić. Nature Communications volume 15, Article number: 4239 (2024) ENZ TALIN-BASED MECHANOSENSORY HYDROGEL Patent WO/2024/100399 Publication Date 16.05.2024 Applicants UNIVERSITY OF KENT [GB]/[GB] Inventors HISCOCK, Jennifer, GOULT, Ben MATERIALS Reengineering of an Artificial Protein Cage for Efficient Packaging of Active Enzymes Yusuke Azuma…Jonathan G. Heddle. Small 2024 May MATERIALS Comparative analysis of folding and unfolding dynamics and free-energy landscapes in homologous cold shock proteins with variable thermal stabilities Zhenyong Xue…Hu Chen. Phys. Rev. Research 6, 023170 – Published 14 May 2024 Engineering Pickering Emulsion with Cell Surface-Displayed Esterase Est7 for Specific and Efficient Synthesis of EPA Monoglyceride Kunpeng Gao…Xiangzhao Mao. ACS Sustainable Chem. Eng. 2024 MATERIALS ENZ Mosaic RBD nanoparticle elicits immunodominant antibody responses across sarbecoviruses Chuanyu Liu…George F. Gao. Cell Reports 2024 May IMMUN MODIFIED COVALENTLY-LINKED PILI AND RECOMBINANT BACTERIA COMPRISING THE SAME Patent WO/2024/092769 Publication Date 10.05.2024 Applicants SHENZHEN INSTITUTE OF ADVANCED TECHNOLOGY Inventors ZHONG, Chao et al. MATERIALS UNIVERSAL ADJUVANT FOR NASAL, ORAL, AND INTRAMUSCULAR DELIVERY OF VACCINES Patent United States of America Publication Date 02.05.2024 Applicants Design-Zyme LLC IMMUN SELF-ADJUVANTING MULTI-PROTEIN COMPLEXES FOR MODULAR VACCINE PRODUCTION Patent WO/2024/097817 Publication Date 10.05.2024 Applicants DESIGN-ZYME LLC [US]/[US] IMMUN Customizable Click Biochemistry Strategy for the Design and Preparation of Glucagon-like Peptide-1 Conjugates and Coagonists Yunchun Zheng…Xiaofeng Yang. Bioconjug Chem. 2024 May 3. Nanobody in a Double “Y”-Shaped Assembly: A Promising Candidate for Lateral Flow Immunoassays Yumeng Xiao…Lichuan Gu. Anal. Chem. 2024 IMMUN Ligand-dependent folding and unfolding dynamics and free energy landscapes of acylphosphatase Li Yuan…Hu Chen. Soft Matter 2024 Apr ENZ Construction of Switch Modules for CAR-T Cell Treatment Using a Site-Specific Conjugation System Tuersunayi Abudureheman…Kai-Ming Chen. Bioconjugate Chem. 2024 IMMUN SpyTag Peptide with Alkoxyl Aspartic Acids for pH-Dependent Activation of the SpyCatcher/Tag System Sonji Che…Koki Makabe. Bioconjugate Chem. 2024 Catalytic Synthesis of (S)-CHBE by Directional Coupling and Immobilization of Carbonyl Reductase and Glucose Dehydrogenase Yadong Wang…Fenghuan Wang. Biomolecules 2024, 14(4), 504 ENZ BUOYANCY ACTIVATED CELL SORTING AND SCALABLE PROTEIN PURIFICATION WITH ENGINEERED GAS VESICLES Patent WO/2024/086802 Publication Date 25.04.2024 LU, Jiaozhi et al. MATERIALS POLYPEPTIDE FUSIONS OR CONJUGATES FOR GENE EDITING Patent WO/2024/086596 Publication Date 25.04.2024 4M GENOMICS INC. ENZ SYNTHETIC INTERMEDIATES FOR UNIVERSAL CHIMERIC ANTIGEN RECEPTOR IMMUNE CELL THERAPIES Patent WO/2024/081824 Publication Date 18.04.2024 Applicants UNIVERSITY OF WASHINGTON [US]/[US] Inventors SELLERS, Drew et al. IMMUN BIOCOMPATIBLE QUANTUM DOT-POLYMER COMPOSITE AND DIAGNOSTIC KIT USING SAME Patent 18276829 Publication Date 11.04.2024 Applicants UNIST Inventors Jongnam Park et al. MATERIALS Detoxifying Cyanides Using Cyanase Enzyme Complexes Composed of Carbonic Anhydrase via Irreversible Covalent Bonds Habin Sun…Jeong Eun Hyeon. J. Agric. Food Chem. 2024, 72, 10, 5318–5324 ENZ Antigen-functionalized turnip mosaic virus nanoparticles increase antibody sensing in saliva. A case study with SARS-CoV-2 RBD Carlos Medrano-Arranz…Daniel A. Truchado. Diagnostic Microbiology and Infectious Disease Volume 109, Issue 3, July 2024, 116298 IMMUN METHODS AND COMPOSITIONS FOR T CELL DIFFERENTIATION Patent WO/2024/076499 Publication Date 11.04.2024 Inventors DALEY, George Q. et al. IMMUN 1000 SYSTEMS, FORMULATIONS AND METHODS FOR GENERATING UNIVERSAL PEPTIDE/MHC COMPLEXES WITH ENGINEERED DISULFIDE CONNECTING THE HEAVY AND LIGHT CHAINS Patent WO/2024/072958 Publication Date 04.04.2024 Inventors SGOURAKIS, Nikolaos et al. IMMUN DIRECTED IN VIVO AFFINITY MATURATION OF ANTIBODIES Patent WO/2024/073744 Publication Date 04.04.2024 Inventors FARZAN, Michael R. et al. IMMUN Overcoming Protein Orientation Mismatch Enables Efficient Nanoscale Light-Driven ATP Production Andrea Marco Amati…Christoph von Ballmoos. ACS Synth. Biol. April 3, 2024 MATERIALS Precision Engineering the Co-Immobilization of Enzymes for Cascade Biocatalysis Zhiyuan Luo…Roger A Sheldon. Angew Chem Int Ed Engl 2024 Mar 31:e202403539. ENZ Proofreading and single-molecule sensitivity in T-cell receptor signaling by condensate nucleation William L White…Hao Yuan Kueh. bioRxiv 2024 Mar IMMUN AN ENZYMATIC SYSTEM FOR PRECISE CELL TARGETING Patent WO/2024/064818 Publication Date 28.03.2024 HOLDEN, Rebecca ENZ AFFINITY CAPTURING AND DIRECTLY DETERMINING STRUCTURES OF PROTEINS AND OTHER MATERIALS ON SUPERPARAMAGNETIC BEADS BY CRYO-ELECTRON MICROSCOPY SINGLE-PARTICLE ANALYSIS WO/2024/064112 Publication Date 28.03.2024 ARIMURA, Yasuhiro et al. MATERIALS Construction of a versatile fusion protein for targeted therapy and immunotherapy Xiu-Song Huang…Cai-Wen Duan. Protein Science 19 March 2024 IMMUN Rational Design of Spontaneous Self-Cyclization Enzymes In Vivo and In Vitro with Improved Thermal Tolerance and Activity Bin Wei…Hao Liang. ACS Catal. 2024, 14, 5469–5480 March 28, 2024 ENZ Intranasal delivery of Salmonella OMVs decorated with Chlamydia trachomatis antigens induces specific local and systemic immune responses Dung T. Huynh…Joen Luirink. Human Vaccines & Immunotherapeutics Volume 20, 2024 IMMUN SpyMask enables combinatorial assembly of bispecific binders Claudia L. Driscoll…Mark R. Howarth. Nature Communications volume 15, Article number: 2403 (2024) IMMUN Molecular density-accelerated binding-site maturation underlies CENP-T-dependent kinetochore assembly Ekaterina V. Tarasovetc…Ekaterina L. Grishchuk. bioRxiv 2024 Rational structure-guided design of a blood stage malaria vaccine immunogen presenting a single epitope from PfRH5 Thomas E. Harrison…Matthew K. Higgins. bioRxiv 2024 IMMUN Designed mosaic nanoparticles enhance cross-reactive immune responses in mice Eric Wang…Arup K. Chakraborty. bioRxiv 2024 IMMUN Architecture and flexibility of native kinetochores revealed by structural studies utilizing a thermophilic yeast Daniel J. Barrero…Sue Biggins. bioRxiv 2024 METHODS OF PRODUCING EXTRACELLULAR VESICLES Patent Application Number 18246304 Publication Date 14.03.2024 Applicants LONZA SALES AG Nanoparticle vaccines based on the receptor binding domain of porcine deltacoronavirus elicit robust protective immune responses in mice Yuanhong Wang…Lingxue Yu. Front. Immunol., 14 March 2024 IMMUN A cVLP-Based Vaccine Displaying Full-Length PCSK9 Elicits a Higher Reduction in Plasma PCSK9 Than Similar Peptide-Based cVLP Vaccines Louise Goksøyr…Adam F. Sander. Vaccines 2023, 11(1), 2; IMMUN NANOPARTICLE-DERIVED VACCINES AGAINST POXVIRUSES, AND METHODS FOR MAKING AND USING THE SAME Patent WO/2024/049990 Publication Date 07.03.2024 Inventors MOSS, Bernard et al. IMMUN Rapid and Convenient Single-Chain Variable Fragment-Employed Electrochemical C-Reactive Protein Detection System Daimei Miura… Ryutaro Asano. Int. J. Mol. Sci. 2024, 25(5), 2859 Site-directed immobilization of enzymes on nanoparticles using self-assembly systems Haili Zhou…Fei Peng. Bioresour Technol 2024 Feb 28:397:130505. ENZ MagIC-Cryo-EM: Structural determination on magnetic beads for scarce macromolecules in heterogeneous samples Yasuhiro Arimura…Hironori Funabiki. bioRxiv 2024 Feb HIGH THROUGHPUT PEPTIDE IDENTIFICATION USING CONJUGATED BINDERS AND KINETIC ENCODING US patent 18466543 Publication Date 15.02.2024 Applicants Encodia, Inc. SEQUENCING Denaturing purifications demonstrate that PRC2 and other widely reported chromatin proteins do not appear to bind directly to RNA in vivo Jimmy K. Guo…Mitchell Guttman. Molecular Cell 21 February 2024 Mosaic sarbecovirus vaccination elicits cross-reactive responses in pre-immunized animals Alexander A. Cohen…Pamela J. Bjorkman. bioRxiv 2024 Feb IMMUN 975 LENGTH-ADAPTED CHIMERIC ANTIGEN RECEPTORS AND LENGTH-ADAPTED ACCESSORY RECEPTORS FOR IMPROVED CAR T CELL ACTIVATION Patent WO/2024/033642 Publication Date 15.02.2024 DUSHEK, Omer IMMUN Development and Immunological Evaluation of a Multiantigen Thermostable Nanovaccine Adjuvanted with T-Cell-Activating Scaffold for African Swine Fever Lidan Sun…Zeliang Chen. ACS Appl. Bio Mater. 2024 Feb IMMUN Irreversible light-activated SpyLigation mediates split-protein assembly in 4D Brizzia G. Munoz-Robles & Cole A. DeForest. Nature Protocols 2024 Jan MATERIALS OptoREACT: Optogenetic Receptor Activation on Nonengineered Human T Cells Anja Armbruster…Maximilian Hörner. ACS Synth. Biol. 2024 Feb IMMUN High-Level Secretory Production of Recombinant E2-Spy Antigen Protein via Combined Strategy in Pichia pastoris Bingkun Li…Ding Li. Fermentation 2024 Feb IMMUN Flow-Induced Microfluidic Assembly for Advanced Biocatalysis Materials Phillip Lemke…Christof M. Niemeyer. Advanced Functional Materials 24 January 2024 MATERIALS ENZ Nanoengineering Carboxysome Shells for Protein Cages with Programmable Cargo Targeting Tianpei Li…Lu-Ning Liu. ACS Nano 2024 Feb MATERIALS ENZ Active Template Synthesis of Protein [n]Catenanes Using Engineered Peptide–Peptide Ligation Tools Fan Zhang…Wen-Bin Zhang. CCS Chemistry Feb 2024 MATERIALS Development of plug-and-deliverable intracellular protein delivery platforms based on botulinum neurotoxin Seong Guk Park… Sebyung Kang. Int J Biol Macromol 2024 Jan 23;261(Pt 1):129622. ENZ Mechanically Tunable, Compostable, Healable and Scalable Engineered Living Materials Avinash Manjula-Basavanna…Anna Duraj-Thatte. bioRxiv 2024 Feb MATERIALS VIRAL PARTICLES RETARGETED TO TRANSFERRIN RECEPTOR 1 Patent WO/2024/026494 Publication Date 01.02.2024 REGENERON PHARMACEUTICALS, INC. [US]/[US] IMMUN De novo design of modular protein hydrogels with programmable intra- and extracellular viscoelasticity Rubul Mout…David Baker. PNAS 2024 Jan MATERIALS Exploration and Application of DNA-Binding Proteins to Make a Versatile DNA–Protein Covalent-Linking Patch (D-Pclip): The Case of a Biosensing Element Erika Komiya…Ryutaro Asano. J. Am. Chem. Soc. 2024 Jan Enhancing antibody responses by multivalent antigen display on thymus-independent DNA origami scaffolds Eike-Christian Wamhoff…Mark Bathe. Nature Communications volume 15, Article number: 795 (2024) MATERIALS IMMUN Spontaneous and site-specific immobilization of PNGase F via spy chemistry Liang Zhang…Song Wang. RSC Advances 2024 Jan ENZ Cryo-EM analysis on magnetic beads for scarce macromolecules in heterogeneous samples Yasuhiro Arimura…Hironori Funabiki. bioRxiv 2024 Jan MATERIALS Engineering multivalent Fc display for FcγR blockade Ekaterina Petrova…Ana I. Teixeira. bioRxiv 2024 Jan IMMUN Discovery and engineering of the antibody response against a prominent skin commensal Djenet Bousbaine…Michael A. Fischbach. Nature 2024 Dec and bioRxiv 2024 Jan IMMUN Enhancing humoral and mucosal immune response of PED vaccine candidate by fusing S1 protein to nanoparticle multimerization Minghui Li…Gaiping Zhang. Veterinary Microbiology19 January 2024, 110003 IMMUN Photoactivatable Nanobody Conjugate Dimerizer Temporally Resolves Tiam1-Rac1 Signaling Axis Chengjian Zhou…Xi Chen. Advanced Science. 15 January 2024 OMPG VARIANTS Patent Application Number 18449904 Publication Date 11.01.2024 Applicants Roche Sequencing Solutions, Inc. SEQUENCING GENETICALLY ENGINEERED RECOMBINANT PROTEINS FOR FUNCTIONAL REGENERATIVE TISSUE SCAFFOLDS Patent Application Number 18365648 Publication Date 11.01.2024 Inventors Minkyu Kim et al. MATERIALS MOLECULAR PEPTIDE MUTANT Patent Application Number 18255108 Publication Date 04.01.2024 Applicants NANJING TECH UNIVERSITY Potent immune responses against thermostable Foot-and-Mouth disease virus VP1 nanovaccine adjuvanted with polymeric thermostable scaffold Yuanli Peng…Zeliang Chen. Vaccine 13 January 2024 IMMUN Affinity-assisted covalent self-assembly of PduQ-SpyTag and Nox-SpyCatcher to construct multi-enzyme complexes on the surface of magnetic microsphere modified with chelated Ni2+ Mianxing Luo…Guo Chen. International Journal of Biological Macromolecules 11 January 2024 ENZ 950 Light-based juxtacrine signaling between synthetic cells Hossein Moghimianavval…Allen P Liu. bioRxiv 2024 Jan Reengineering of an artificial protein cage for efficient packaging of active enzymes Yusuke Azuma…Jonathan G. Heddle. bioRxiv 2023 Dec ENZ Enhancing immune responses of ESC-based TAA cancer vaccines with a novel OMV delivery system Meiling Jin…Jian-Dong Huang. Journal of Nanobiotechnology volume 22, Article number: 15 (2024) IMMUN Hidden route of protein damage through confined oxygen gas Seoyoon Kim…Duyoung Min. bioRxiv 2024 Jan MATERIALS Preclinical Development of a Stabilized RH5 Virus-Like Particle Vaccine that Induces Improved Anti-Malarial Antibodies Lloyd D W King…Mark Howarth, Sumi Biswas, Simon J Draper. Cell Reports Medicine Volume 5, Issue 7, 16 July 2024, 101654 and bioRxiv 2024 Jan IMMUN Cleavage-intermediate Lassa virus trimer elicits neutralizing responses, identifies neutralizing nanobodies, and reveals an apex-situated site-of-vulnerability Jason Gorman…Peter D. Kwong. Nature Communications volume 15, Article number: 285 (2024) IMMUN KARI NANOPARTICLE Patent WO/2024/006532 Publication Date 04.01.2024 OLMEDILLAS, Eduardo et al. Extraordinary Titer and Broad Anti-SARS-CoV-2 Neutralization Induced by Stabilized RBD Nanoparticles from Strain BA.5 Z. Wang…S. Wang. Vaccines 2024 IMMUN SpyDirect: A Novel Biofunctionalization Method for High Stability and Longevity of Electronic Biosensors Keying Guo…Sahika Inal. Advanced Science 31 December 2023 MATERIALS Display of PETase on the Cell Surface of Escherichia coli Using the Anchor Protein PgsA Takuma Yamashita…Hiroyasu Ogino. Applied Biochemistry and Biotechnology Jan 2024 ENZ Lipid nanodisc scaffold and size alter the structure of a pentameric ligand-gated ion channel Vikram Dalal…Wayland W. L. Cheng. Nature Communications volume 15, Article number: 25 (2024) Molecular Engineering of a Spider Silk and Mussel Foot Hybrid Protein Gives a Strong and Tough Biomimetic Adhesive Yin Yin…Markus B. Linder. Advanced Materials Interfaces 02 January 2024 MATERIALS METHODS FOR SPORE DISPLAY Patent WO/2023/245018 Publication Date 21.12.2023 Inventors NICKS, Trevor et al. MATERIALS IMMUN The SpyCatcher-SpyTag interaction mediates tunable anti-tumor cytotoxicity of NK cells. Guo C…Zhu W. Mol Immunol. 2023 Dec 20;165:11-18. IMMUN Ligand-Mediated Mechanical Enhancement in Protein Complexes at Nano- and Macro-Scale Samuel Kim…Minkyu Kim. ACS Appl. Mater. Interfaces 2023 MATERIALS De novo Semi-synthetic Platform for Monitoring Protein degradation in Live Cells Mahdi Hasan…Ashraf Brik. Chembiochem 2023 Nov 30:e202300731. Protein cage nanoparticle-based NK cell-engaging nanodrones (NKeNDs) effectively recruit NK cells to target tumor sites and suppress tumor growth Seong Guk Park…Sebyung Kang. Nanotoday 2024 Feb IMMUN Engineered immunogens to elicit antibodies against conserved coronavirus epitopes A. Brenda Kapingidza…Mihai L. Azoitei. Nature Communications volume 14, Article number: 7897 (2023) IMMUN METHODS AND COMPOSITIONS FOR DETERMINING THE ANTIGEN SPECIFICITY OF T CELLS Patent WO/2023/235761 Publication Date 07.12.2023 Inventors HACOHEN, Nir BAKALAR, Matthew IMMUN UNIVERSAL ADJUVANT FOR NASAL, ORAL, AND INTRAMUSCULAR DELIVERY OF VACCINES Patent WO/2023/230241 Publication Date 30.11.2023 DESIGN-ZYME LLC [US]/[US] IMMUN Encapsulation of AAV into protein vault nanoparticles as a novel solution to gene therapy's neutralizing antibody problem Logan Thrasher Collins…David Curiel. bioRxiv 2023 Nov IMMUN MATERIALS Accelerating the design of pili-enabled living materials using an integrative technological workflow Yuanyuan Huang…Chao Zhong. Nature Chemical Biology (2023) MATERIALS Enhancing anti-viral neutralization response to immunization with HIV-1 envelope glycoprotein immunogens Shamim Ahmed…Alon Herschhorn. npj Vaccines volume 8, Article number: 181 (2023) IMMUN Free Energy Landscape of Type III Fibronectin Domain with Identified Intermediate State and Hierarchical Symmetry Hao Sun…Hu Chen. Phys. Rev. Lett. 131, 218402 – Published 22 November 2023 MATERIALS Dimer stabilization by SpyTag/SpyCatcher coupling of the reductase domains of a chimeric P450 BM3 monooxygenase from Bacillus spp. improves its stability, activity and purification Arabella Essert, Kathrin Castiglione. ChemBioChem 2023 Nov ENZ 925 A candidate nanoparticle vaccine comprised of multiple epitopes of the African swine fever virus elicits a robust immune response Jinxing Song…Gaiping Zhang. Journal of Nanobiotechnology volume 21, Article number: 424 (2023) IMMUN VECTORS AND METHODS FOR IN VIVO ANTIBODY PRODUCTION Patent WO/2023/220603 Publication Date 16.11.2023 REGENERON PHARMACEUTICALS, INC. [US]/[US] IMMUN Study on the properties of a dual-system-based protein scaffold for orthogonal self-assembly Meng Zhang…Jun Zhao. International Journal of Biological Macromolecules16 November 2023, 127946 MATERIALS Development of a Tag/Catcher-mediated capsid virus-like particle vaccine presenting the conserved Neisseria gonorrhoeae SliC antigen that blocks human lysozyme Fabian G Martinez…Aleksandra E Sikora. Infect Immun 2023 Nov 2:e0024523. IMMUN Protein nanoscaffold enables programmable nanobody-luciferase immunoassembly for sensitive and simultaneous detection of aflatoxin B1 and ochratoxin A Shaowen Wu…Shijuan Yan. J Hazard Mater 2023 Oct 5:462:132701. ENZ A novel protein purification scheme based on salt inducible self-assembling peptides Guang Zeng…Zhanglin Lin. Microbial Cell Factories volume 22, Article number: 224 (2023) A BACTERIOPHAGE-BASED, NEEDLE AND ADJUVANT-FREE, MUCOSAL COVID-19 VACCINE Patent WO/2023/209556 Publication Date 02.11.2023 Inventors RAO, Venigalla B. et al. IMMUN VARIANTS OF THE GAIN DOMAIN OF ADHESION G PROTEIN-COUPLED RECEPTORS Patent WO/2023/213993 Publication Date 09.11.2023 Inventors MARFOGLIA, Matthieu et al. METHOD FOR BIOSYNTHESIS OF PROTEIN HETEROCATENANE Patent United States of America Application Number 17999377 Publication Date 02.11.2023 Inventors Wenbin ZHANG et al MATERIALS A modular approach to enhancing cell membrane-coated nanoparticle functionality using genetic engineering Nishta Krishnan…Liangfang Zhang. Nature Nanotechnology (2023) Oct IMMUN A nanoparticle vaccine displaying the ookinete PSOP25 antigen elicits transmission-blocking antibody response against Plasmodium berghei Guixiang Yao…Yaming Cao. Parasites & Vectors volume 16, Article number: 403 (2023) IMMUN Molecular imaging of bacterial outer membrane vesicles based on bacterial surface display Dávid Szöllősi…Krisztián Szigeti. Scientific Reports volume 13, Article number: 18752 (2023) CONJUGATE SYSTEMS AND METHODS OF USE THEREOF Patent Application Number 18301228 Publication Date 19.10.2023 Applicants David Curiel et al. IMMUN MODIFIED VIRAL PARTICLES FOR GENE THERAPY US patent Application Date 11.05.2023 Publication Date 26.10.2023 Inventors Paul Alexander HEPPENSTALL IMMUN LONG LIFETIME ALPHA-HEMOLYSIN NANOPORES US patent Application Number 18171969 Publication Date 19.10.2023 Applicants Roche Sequencing Solutions SEQUENCING Engineered Reversible Inhibition of SpyCatcher Reactivity Enables Rapid Generation of Bispecific Antibodies Christian Hentrich…Francisco Ylera. Nature Communications volume 15, Article number: 5939 (2024) and bioRxiv 2023 Oct IMMUN Mechanical disengagement of the cohesin ring Martina Richeldi…Maxim I. Molodtsov. Nature Structural & Molecular Biology (2023) Oct MATERIALS Thermostable T Cell Multiepitope Nanoparticle Antigens Inducing Potent Immune Responses against the Swine Fever Virus Lidan Sun…Zeliang Chen. ACS Infect. Dis. 2023, Oct IMMUN Toward Minimal Transcription–Translation Machinery Ting Wang and Yuan Lu. ACS Synth. Biol. 2023 Oct ENZ An Antibiotic Degrading Engineered Living Material Platform to Combat Environmental Antibiotic Resistance Gokce Ozkul…Ozgur Safak Seker. bioRxiv 2023 Oct MATERIALS HIGH THROUGHPUT PEPTIDE IDENTIFICATION USING CONJUGATED BINDERS AND KINETIC ENCODING Patent WO/2023/201365 Publication Date 19.10.2023 ENCODIA, INC. [US]/[US] SEQUENCING Engineering of an Optogenetic T Cell Receptor Compatible with Fluorescence-Based Readouts Vincent Idstein…Wolfgang W. Schamel. ACS Synth. Biol. 2023 Oct IMMUN Biological Applications of Synthetic Binders Isolated from a Conceptually New Adhiron Library Claudia D’Ercole…Ario de Marco. Biomolecules 2023, 13(10), 1533 Synthetic Par polarity induces cytoskeleton asymmetry in unpolarized mammalian cells JL Watson…E Derivery. Cell Volume 186, Issue 21, 12 October 2023, Pages 4710-4727.e35 Unveiling Mechanical Activation: GAIN Domain Unfolding and Dissociation in Adhesion GPCRs Chaoyu Fu…Jie Yan. Nano Lett. 2023 Oct MATERIALS Publications 601-900 here. 301-600 publications here. 1-300 publications here.