ADDRESS Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD UK
CONTACT e: t: 01223 334176
2023 Mark Howarth. All rights reserved.
Papers and Patents using Spy technology Many of these publications don’t come up from a Pubmed search or citation search, so it should be useful to bring them all together and hyperlinked (now >800 publications). Patents may be omitted if there is a journal paper covering very similar work. Ones with particular relevance to IMMUNOLOGY, MATERIALS (polymers or mechanobiology), IMAGING , SEQUENCING or ENZYMES are marked. For an overview, please see our open-access review “Power to the Protein” from 2020. SpyBank is a database of >1,000 Tag/Catcher aa sequences to download (Excel file), from publications below. SpyBank includes includes expression organism, Tag/Catcher location (N-terminal, internal, C-terminal) and annotated linkers, as a reference for groups creating their own fusions. SpyBank itself is described in: “Insider information on successful covalent protein coupling with help from SpyBank. Keeble AH, Howarth M. Meth Enz 2019 PDF”. (See note on codon usage at Reagents page). Most recent below. Publications 301-600 here. Publications 1-300 here. Ligand requirements for immunoreceptor triggering Michael I. Barton…Anton van der Merwe. bioRxiv 2023 Sept IMMUN Engineered Immunogens to Elicit Antibodies Against Conserved Coronavirus Epitopes Brenda Kapingidza…Mihai L. Azoitei. bioRxiv 2023 September IMMUN Bioluminescent detection of viral surface proteins using branched multivalent protein switches Alexander Grawe…Maarten Merkx. bioRxiv 2023 Sept IMMUN MATERIALS Engineered Escherichia coli Consortia Function in a Programmable Pattern for Multiple Enzymatic Biosynthesis Wenxue Zhang…Ping Wang. ACS Appl. Mater. Interfaces 2023 September ENZ Recording Binding Information Directly into DNA-Encoded Libraries Using Terminal Deoxynucleotidyl Transferase Lukas A. Schneider…Dennis Gillingham. JACS 2023 September ENZ Snoopligase-catalyzed molecular glue enables efficient generation of hyperoligomerized TRAIL variant with enhanced antitumor effect T She…X Lu. Journal of Controlled Release. September 2023, Pages 856-870 MATERIALS Molecular basis of product recognition during PIP5K-mediated production of PI(4,5)P2 with positive feedback Benjamin R. Duewell…Scott D. Hansen. bioRxiv 2023 September ENZ The molecular reach of antibodies determines their SARS-CoV-2 neutralisation potency Anna Huhn…Omer Dushek. bioRxiv 2023 Sept IMMUN AP profiling resolves co-translational folding pathway and chaperone interactions in vivo Xiuqi Chen…Christian M. Kaiser. bioRxiv 2023 September In-situ Single-Molecule Investigations of the Impacts of Biochemical Perturbations on Conformational Intermediates of Monomeric α-Synuclein Wenmao Huang…Jie Yan. bioRiv 2023 Sept MATERIALS HIV-1 binds dynein directly to hijack microtubule transport machinery Somayesadat Badieyan…Michael A. Cianfrocco. bioRxiv 2023 Sept MATERIALS A platform for dissecting force sensitivity and multivalency in actin networks Joseph T. Levin…Gregory M. Alushin. bioRxiv 2023 Aug MATERIALS Rationally designed multimeric nanovaccines using icosahedral DNA origami for molecularly controlled display of SARS-CoV-2 receptor binding domain Qingqing Feng…Xiao Zhao. bioRiv 2023 Aug IMMUN MATERIALS Engineering Protein Nanoparticles Functionalized with an Immunodominant Coxiella burnetii Antigen to Generate a Q Fever Vaccine Aaron Ramirez…Szu-Wen Wang. Bioconjugate Chem. 2023 September IMMUN Compact and modular bioprobe: Integrating SpyCatcher/SpyTag recombinant proteins with zwitterionic polymer-coated quantum dots Sunghwan Kim…Jongnam Park. J Colloid Interface Sci 2023 Aug 6;652(Pt A):184-194. 875 SpyCombinator Assembly of Bispecific Binders Claudia L Driscoll…Mark Howarth. bioRxiv 2023 Sept Neoantigen-Based Nanovaccine In Combination with Immune Checkpoint Inhibitors Abolish Postsurgical Tumor Recurrence and Metastasis Peng Zheng…Yanbing Ma. Small 2023 Aug IMMUN GENETICALLY CONTROLLED NANOSCOPY CONTRAST-GENERATING UNITS, GENETICALLY CONTROLLED STRUCTURAL ELEMENTS, GENETICALLY CONTROLLED SCAFFOLDS, NANOBIOMATERIAL BASED THEREON, AND USE THEREOF IN NANOSOCOPY METHODS Patent WO/2023/156638 Publication Date 24.08.2023 Inventors WESTMEYER, Gil Gregor et al. MATERIALS IMAGING METHODS AND COMPOSITIONS FOR TREATING OR AMELIORATING CARDIAC MUSCLE ARRHYTHMIAS AND SKELETAL MUSCLE TREMORS Patent WO/2023/159234 Publication Date 24.08.2023 Inventors HARRIS, Samantha, P. MATERIALS A platform for dissecting force sensitivity and multivalency in actin networks Joseph T. Levin…Gregory M. Alushin. bioRxiv 2023.08.15.553463 MATERIALS Myddosome clustering in IL-1 receptor signaling regulates the formation of an NF-kB activating signalosome Fakun Cao…Marcus J Taylor. EMBO Reports (2023)e57233 IMMUN Affinity-induced covalent protein-protein ligation via the SpyCatcher-SpyTag interaction Jacob O. Fierer…Edward A. Bayer. Green Carbon 17 July 2023 ENZ Visible light-induced specific protein reaction delineates early stages of cell adhesion Rolle Rahikainen…Mark Howarth. bioRxiv 2023.07.21.549850 MATERIALS IMAGING ANTIBODIES AND ANTIBODY FRAGMENTS AND ANALOGUES SPECIFIC FOR CHONDROITIN SULFATE Patent WO/2023/148398 Publication Date 10.08.2023 Applicants VAR2 PHARMACEUTICALS APS [DK]/[DK] IMMUN ANTIBODIES FOR CORONAVIRUS AND USES THEREOF Patent US Application Number 18060814 Publication Date 10.08.2023 Pamela J. Bjorkman et al. IMMUN METHODS FOR GENERATING NUCLEIC ACID ENCODED PROTEIN LIBRARIES AND USES THEREOF Patent WO/2023/150742 Publication Date 10.08.2023 Inventors ABATE, Adam et al. Self-assembled multienzyme complex facilitates synthesis of glucosylglycerol from maltodextrin and glycerol Xinming Sun…Yuanxia Sun. J Sci Food Agric 2023 Aug 8. ENZ Harnessing split fluorescent proteins in modular protein logic for advanced whole-cell detection Ping-Hen Lim…Yi-Chun Yeh. Analytica Chimica Acta 1275, 22 Sept 2023, 341593 IMAGING Thermostability enhancement of polyethylene terephthalate degrading PETase using self- and nonself-ligating protein scaffolding approaches Barindra Sana…John F Ghadessy. Biotechnol Bioeng 2023 Aug 9. ENZ Toward Selective Synthesis of Protein Olympiadanes via Orthogonal Active Templates in One Step Fan Zhang…Wen-Bin Zhang. CCCS Chem 2023 Jul MATERIALS KITS FOR ANALYSIS USING NUCLEIC ACID ENCODING AND/OR LABEL US patent Application Number 18166461, Application Date 08.02.2023. Encodia, Inc. Nanoparticle-based multivalent human antibodies offer potent and broad neutralization against Omicron sublineages Yizhen Wang…Ningshao Xia. Signal Transduction and Targeted Therapy volume 8: 284 (2023) IMMUN Raman Spectroscopy Combined with Malaria Protein for Early Capture and Recognition of Broad-Spectrum Circulating Tumor Cells Xinning Liu…Jinbo Yang. Int. J. Mol. Sci. 2023, 24(15), 12072 IMAGING In vivo fate of systemically administered encapsulin protein nanocages and implications for their use in targeted drug delivery Claire Rennie…Andrew Care. bioRxiv 2023 July MATERIALS Truly tiny acoustic biomolecules for ultrasound imaging and therapy Bill Ling…Mikhail G. Shapiro. bioRxiv 2023 June MATERIALS IMAGING Cell Cycle Dependent Orchestration of Surface Layer Biogenesis in Caulobacter crescentus Matthew Herdman…Tanmay A.M. Bharat. bioRxiv 2023 June MATERIALS De novo design of modular protein hydrogels with programmable intra- and extracellular viscoelasticity Rubul Mout…David Baker. bioRxiv 2023 June MATERIALS POLYPEPTIDE TERMINAL BINDERS AND USES THEREOF US Patent Application Number 17539033 Publication Date 13.07.2023 Encodia, Inc. ENZ SEQUENCING MULTIPLEXED IMMUNOSIGNAL AMPLIFICATION USING HYBRIDIZATION CHAIN REACTION-BASED METHOD US Patent Application Number 18045811 Publication Date 13.07.2023 Rui LIN Minmin LUO ENZ Construction of metal–organic framework-based multienzyme system for l-tert- leucine production Ru Wang…Feng Xue. Bioprocess and Biosystems Engineering 2023 July MATERIALS ENZ Bacterial Surface-Assembled Chitinosome for Dismantling Chitin into N-Acetyl Glucosamine Chao Du…Guimin Zhang. ACS Sustainable Chem. Eng. 2023 July MATERIALS ENZ 850 CATENATED VAR2CSA RECOMBINANT PROTEIN, PREPARATION METHOD THEREFOR, AND USE THEREOF Patent WO/2023/130667 Publication Date 13.07.2023 CAS LAMVAC (GUANGZHOU) BIOMEDICAL TECHNOLOGY CO., LTD [CN]/[CN] IMMUN Long-term retention of antigens in germinal centers is controlled by the spatial organization of the follicular dendritic cell network Ana Martínez-Riaño…Pavel Tolar. Nature Immunology (2023 July) IMMUN Malaria Biomimetic for Tumor Targeted Drug Delivery Jessica Pihl…Ali Salanti. ACS Nano July 12, 2023 IMMUN Cooperation between bHLH transcription factors and histones for DNA access Alicia K. Michael…Nicolas H. Thomä. Nature 2023 Jul Simultaneous Mapping of DNA Binding and Nucleosome Positioning with SpLiT-ChEC Orion G. B. Banks…Laura E. McKnight. bioRxiv Jul 2023 ENZ METHOD FOR PRODUCING VIRUS-FILTERING MODIFIED LECTIN-BINDING MODIFIED BIOFILM Patent WO/2023/128981 Publication Date 06.07.2023 BILKENT UNIVERSITESI Inventors SEKER, Urartu Ozgur Safak et al. MATERIALS The position of Spy Tag/Catcher system in hepatitis B core protein particles affects the immunogenicity and stability of the synthetic vaccine Y Sheng…S Zhang. Vaccine 28 June 2023 IMMUN T Cell Activating Thermostable Self-Assembly Nanoscaffold Tailored for Cellular Immunity Antigen Delivery Jinsong Zhang…Zeliang Chen. Advanced Science 3 July 2023 MATERIALS IMMUN IMMOBILISED ENZYME Patent WO/2023/118901 Publication Date 29.06.2023 FABRICNANO LIMITED [GB]/[GB] MATERIALS ENZ METHODS FOR BALANCING ENCODING SIGNALS OF ANALYTES Patent WO/2023/122698 Publication Date 29.06.2023 ENCODIA, INC. [US]/[US] MATERIALS ENZ SEQUENCING Biocatalytic Foams from Microdroplet-Formulated Self-Assembling Enzymes Julian S Hertel…Christof M Niemeyer. Adv Mater 2023 Jun 26;e2303952. MATERIALS ENZ Bioinspired Amyloid Fibril-Based Hydrogel with Engineering Programable Functionalities for Diverse Applications Weiqiang Wang…Honghua Ge. Advanced Functional Materials 26 June 2023 MATERIALS An RBD virus-like particle vaccine for SARS-CoV-2 induces cross-variant antibody responses in mice and macaques Yuanyuan Li…Jing Jin. Signal Transduction and Targeted Therapy volume 8, Article number: 173 (2023) IMMUN Self-assembled ferritin nanoparticles displaying PcrV and OprI as an adjuvant-free Pseudomonas aeruginosa vaccine Yuhang Li…Jiang Gu. Front. Immunol., 21 June 2023 IMMUN Sneaking in SpyCatcher using cell penetrating peptides for in vivo imaging James Tyler…Behzad Rad. Nanotechnology 2023 June IMAGING Using CombiCells, a platform enabling titration and combinatorial display of cell surface ligands, to investigate the sensitivity and costimulatory requirements of TCRs and CARs Ashna Patel…Omer Dushek. bioRxiv 2023 Jun IMMUN Designed Multifunctional Spider Silk Enabled by Genetically Encoded Click Chemistry Bojing Jiang…Fei Sun. Advanced Functional Materials 15 June 2023 MATERIALS Preclinical immunogenicity and protective efficacy of a SARS-CoV-2 RBD-based vaccine produced with the thermophilic filamentous fungal expression system Thermothelomyces heterothallica C1 Mariana Gonzalez-Hernandez…Albert D. M. E. Osterhaus. Front. Immunol., 09 June 2023 IMMUN Modification of Bacterial Microcompartments with Target Biomolecules via Post-Translational SpyTagging David Mark Beal…Martin Warren. Mater. Adv., 2023 June MATERIALS SWITCHABLE POLYPEPTIDE AND ITS USE FOR GENTLE AFFINITY PURIFICATION Patent WO/2023/099876 Publication Date 08.06.2023 HOWARTH, Mark, ANUAR, Irsyad Khairil, RAHIKAINEN, Rolle ANTIBODIES FOR CORONAVIRUSES AND USES THEREOF Patent WO/2023/102476 Publication Date 08.06.2023 BJORKMAN, Pamela J. et al. IMMUN RATIONALLY DESIGNED IMMUNOGENS Patent WO/2023/102571 Publication Date 08.06.2023 SCHMIDT, Aaron, G. et al. IMMUN Design and Evolution of Enhanced Peptide–Peptide Ligation for Modular Transglutaminase Assembly Anthony H. Keeble, Dominic P. Wood, and Mark Howarth. Bioconjugate Chem. 2023 June ENZ A universal reagent for detection of emerging diseases using bioengineered multifunctional yeast nanofragments Junrong Li…Matt Trau. Nature Nanotechnology (2023) June MATERIALS 825 Catalyst-free thiazolidine formation chemistry enables the facile construction of peptide/protein-cell conjugates (PCCs) at physiological pH Xiang-Quan Liu…Xiaobao Bi. Chem Sci. 2023 June MATERIALS Chemically cross-linked hydrogels from repetitive protein arrays Rossana Boni…Lynne Regan. J Struct Biol 2023 May 26;215(3):107981. MATERIALS SpyTag/Catcher display of influenza M2e peptide on norovirus-like particle provides stronger immunization than direct genetic fusion Vili Lampinen…Minna M. Hankaniemi. Front. Cell. Infect. Microbiol. Volume 13 - 2023 | doi: 10.3389/fcimb.2023.1216364 IMMUN A novel self-purified auxiliary protein enhances the lichenase activity towards lichenan for biomass degradation Rui Zhou…Guangya Zhang. Applied Microbiology and Biotechnology (2023) ENZ IMMOBILISED ENZYME Patent WO/2023/094823 Publication Date 01.06.2023 FABRICNANO LIMITED ENZ Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling EJ Longarini…I Matic. Mol Cell May 2023 METHODS AND COMPOSITIONS FOR DELIVERY OF MOLECULES AND COMPLEXES TO REACTION SITES US patent 17988622 Application Date 16.11.2022 Publication Date 25.05.2023 Pacific Biosciences of California, Inc. SEQUENCING THERMOSTABLE POLYMERASE VARIANTS Patent WO/2023/089175 Publication Date 25.05.2023 ROCHE DIAGNOSTICS GMBH [DE]/[DE](DE) ENZ DIRECTLY FUNCTIONALIZED ELECTROCHMICAL TRANSISTEORS, AND CONVECTION DRIVEN ULTRA- RAPID DETECTION OF BIOMARKERS USING TRANSISTORS Patent WO/2023/089569 Publication Date 25.05.2023 INAL, Sahika et al. MATERIALS RAPID PRODUCTION OF BISPECIFIC ANTIBODIES FROM OFF- THE-SHELF IGGS WITH HIGH YIELD AND PURITY Patent WO/2023/092106 Publication Date 25.05.2023 LINGHAN, Mei et al. COMPOSITIONS THAT REDUCE TEMPLATE THREADING INTO A NANOPORE Patent US Application Number 17817480 Application Date 04.08.2022 Roche Sequencing Solutions, Inc. SEQUENCING METHODS FOR BARCODING MACROMOLECULES IN INDIVIDUAL CELLS Patent WO/2023/086847 Publication Date 19.05.2023 ENCODIA, INC. [US]/[US] SEQUENCING Different Strategies Affect Enzyme Packaging into Bacterial Outer Membrane Vesicles Scott N. Dean…Gregory A. Ellis. Bioengineering 2023 ENZ Phase-Separated Synthetic Organelles Based on Intrinsically Disordered Protein Domain for Metabolic Pathway Assembly in Escherichia coli Li Wan…Wanmeng Mu. ACS Nano May 16, 2023 MATERIALS Biosynthesis and Immunological Evaluation of a Dual-Antigen Nanoconjugate Vaccine against Brucella melitensis Jing Huang…Li Zhu. Engineering 12 May 2023 IMMUN Improving the specific activity and stability of alkaline pectinase PEL3 through SpyTag/SpyCatcher cyclization Chao Du…Guimin Zhang. Biotech Lett May 2023 ENZ Asymmetric Assembly and Self-Adjuvanted Antigen Delivery Platform for Improved Antigen Uptake and Antitumor Effect Jianghua Yang…Zeliang Chen. Bioconjugate Chem. 2023 IMMUN SARS-CoV-2 RBD dimers elicit response comparable to VLPs in mice J. Love…Sergio Rodriguez-Aponte. ResearchSquare 2023 May IMMUN VIRAL PARTICLES RETARGETED TO SKELETAL MUSCLE Patent WO/2023/081850 Publication Date 11.05.2023 REGENERON PHARMACEUTICALS, INC. [US]/[US] cis-B7:CD28 interactions at invaginated synaptic membranes provide CD28 co-stimulation and promote CD8+ T cell function and anti- tumor immunity Yunlong Zhao…Enfu Hui. Immunity 8 May 2023 IMMUN Spy chemistry enables stable protein immobilization on iron oxide nanoparticles with enhanced magnetic properties Chu Shi…Ning Gu. Journal of Materials Science & Technology. 7 May 2023 MATERIALS Preparation of a Klebsiella pneumoniae conjugate nanovaccine using glycol-engineered Escherichia coli Yan Liu…Li Zhu. Microbial Cell Factories. 2023 May IMMUN ENZ Active Template Synthesis of Protein [n]Catenanes Using Engineered Peptide–Peptide Ligation Tools Fan Zhang…Wen-Bin Zhang. CCS Chemistry 5 May 2023 MATERIALS Bispecific T cell-engager targeting oncofetal chondroitin sulfate induces complete tumor regression and protective immune memory in mice Nanna Skeltved…Morten A. Nielsen. Journal of Experimental & Clinical Cancer 42, Article number: 106 (2023) IMMUN Intranasal VLP-RBD vaccine adjuvanted with BECC470 confers immunity against Delta SARS-CoV-2 challenge in K18-hACE2-mice Katherine S Lee…F. Heath Damron. bioRxiv Apr 2023 IMMUN 800 Multiparametric domain insertional profiling of Adeno-Associated Virus VP1 Mareike D. Hoffmann…Daniel Schmidt. bioRxiv Apr 2023 Highly efficiency production of D-allulose from inulin using curli fiber multi-enzyme cascade catalysis Y Chen…L Jiang. International Journal of Biological Macromolecules 241, 30 June 2023, 124468 ENZ Autoinhibitory module underlies species difference in shear activation of von Willebrand factor Nicholas A. Arce…Renhao Li. Journal of Thrombosis and Haemostasis 20, Issue 11, November 2022, Pages 2686-2696 MATERIALS Spatiotemporal functional assembly of split protein pairs through a light-activated SpyLigation Emily R. Ruskowitz…Cole A. DeForest. Nature Chemistry 15 (2023) MATERIALS Genetically encoded barcodes for correlative volume electron microscopy Felix Sigmund…Gil Gregor Westmeyer. Nature Biotechnology (2023) MATERIALS Highly modular hepatitis B virus-like nanocarriers for therapeutic protein encapsulation and targeted delivery to triple negative breast cancer cells Daniel Yur…Wilfred Chen. J Mat Chem B 2023 IMMUN Two-Molecule Force Spectroscopy on Proteins Jiacheng Zuo and Hongbin Li. ACS Nano 2023 April 13 MATERIALS VACCINES AGAINST CORONAVIRUS INFECTION USING LIPID A ANALOGS AS ADVUVANTS WO/2023/060078 Publication Date 13.04.2023 DAMRON, Fredrick Heath et al. IMMUN New botulinum neurotoxin constructs for treatment of chronic pain Charlotte Leese…Bazbek Davletov. Life Science Alliance 2023 April A photoreceptor-based hydrogel with red light-responsive reversible sol-gel transition as transient cellular matrix Maximilian Hörner…Wilfried Weber. bioRxiv 2023 April MATERIALS A highly efficient and specific “grafting to” route for stable protein-polymer conjugates based on Spy chemistry Di Zhang…Hui Zhao. Polymer 7 April 2023 MATERIALS In situ preparation of programmable curli nanomaterials as fine-tuned sustainable supports enabling selective and oriented incorporation of enzymes Hao Dong…Ping Wang. Catalysis Science & Technology 2023 April MATERIALS ENZ Display of multiple proteins on engineered canine parvovirus-like particles expressed in cultured silkworm cells and silkworm larvae Jian Xu…Enoch Y. Park. Front. Bioeng. Biotechnol., 16 February 2023 MATERIALS Bioengineering of air-filled protein nanoparticles by genetic and chemical functionalization Ram Karan…Magnus Rueping. J Nanobiotechnology. 2023 Mar 25;21(1):108. MATERIALS HiBiT-SpyTag: A Minimal Tag for Covalent Protein Capture and Degrader Development Tiffany Tsang…Radosław P. Nowak. ACS Chem. Biol. 2023 ENZ DNA segment capture by Smc5/6 holocomplexes Michael Taschner & Stephan Gruber. Nature Structural & Molecular Biology (2023) METHODS AND PARTICLES FOR MODULATING AN IMMUNE RESPONSE Patent WO/2023/052600 Publication Date 06.04.2023 ADAPTVAC APS [DK]/[DK] IMMUN Construction of "lock-key" biological living material based on double engineered bacteria and its application on intestinal retention in vivo Minghui Zhang…Hanjie Wang. Sheng Wu Gong Cheng Xue Bao 2023 Mar 25;39(3):1163-1174. MATERIALS A multivalent Plasmodium falciparum circumsporozoite protein-based nanoparticle malaria vaccine elicits a robust and durable antibody response against the junctional epitope and the major repeats Geetanjali Pendyala…Ravi S. Kane. Bioeng and Trans Med 28 March 2023 IMMUN ASFV proteins presented at the surface of T7 phages induce strong antibody responses in mice Yuanfang Li…Jinhai Huang. J Virol Methods 2023 Mar 23;114725. IMMUN Macroporous Epoxy-Based Monoliths Functionalized with Anti-CD63 Nanobodies for Effective Isolation of Extracellular Vesicles in Urine by Julia Neumair…Ario de Marco. Int. J. Mol. Sci. 2023, 24(7), 6131 MATERIALS ANTI-VIRUS MOIETY IMMOBILISED IN A MATRIX WO/2023/037119 Patent WO/2023/037119 Publication Date 16.03.2023 SAMATVA RESEARCH CORPORATION [CA]/[CA] IMMUN An Immobilised Silicon-Carbon Bond-Forming Enzyme for Anaerobic Flow Biocatalysis Sabrina Gallus…Kersten S. Rabe. ChemCatChem 21 March 2023 ENZ On-grid purification of electron microscopy samples via a 3D-printed flow-cell IMAGING MATERIALS Kailash Ramlaul…Christopher H. S. Aylett. bioRxiv 2023 March Highly Hydrophobic Films of Engineered Silk Proteins by a Simple Deposition Method Teemu Välisalmi… Markus B. Linder. Langmuir 2023 March MATERIALS 775 Opto-APC: Engineering of cells that display phytochrome B on their surface for optogenetic studies of cell-cell interactions Marissa Russ…Wolfgang W. Schamel. Front. Mol. Biosci., 20 February 2023 Protein Ligation-assisted Reconstitution of Split HRP Fragments for Facile Production of HRP fusion Proteins in E. coli Seungnyeong Heo…Joong-jae Lee. ChemBioChem15 March 2023 ENZ Step-wise mechanical unfolding and dissociation of the GAIN domains of ADGRG1/GPR56, ADGRL1/Latrophilin-1 and ADGRB3/BAI3: insights into the mechanical activation hypothesis of adhesion G protein-coupled receptors Chaoyu Fu…Jie Yan. bioRxiv 2023 March MATERIALS CARRIER MATRIX COMPRISING DODECIN PROTEIN Patent US 17789260 Inventors MARTIN GRININGER et al. MATERIALS Replication Protein A Enhances Kinetics of Uracil DNA Glycosylase on ssDNA and Across DNA Junctions: Explored with a DNA Repair Complex Produced with SpyCatcher/SpyTag Ligation Sharon N. Greenwood…Brian P. Weiser. ChemBioChem 08 March 2023 ENGINEERED COMPOSITIONS Patent WO/2023/028603 Publication Date 02.03.2023 HUMABS BIOMED SA [CH]/[CH] VIR BIOTECHNOLOGY, INC. [US]/[US] GLAXOSMITHKLINE SA [BE]/[BE] IMMUN Multiviral Quartet Nanocages Elicit Broad Anti-Coronavirus Responses for Proactive Vaccinology Rory A Hills…Mark Howarth. bioRxiv 2023 Feb IMMUN Robust single membrane protein tweezers Seoyoon Kim…Duyoung Min. bioRxiv 2023 Feb MATERIALS Piconewton forces mediate GAIN domain dissociation of the latrophilin-3 adhesion GPCR Brian L. Zhong…Alexander R. Dunn. bioRiv 2023 MATERIALS The Ribosome Maturation Factor Rea1 utilizes nucleotide independent and ATP-hydrolysis driven Linker remodelling for the removal of ribosome assembly factors Johan Busselez…Helgo Schmidt. bioRxiv 2023 MATERIALS Docking Domain Engineering in a Modular Polyketide Synthase and its Impact on Structure and Function Lynn Buyachuihan…Martin Grininger. bioRxiv 2023 ENZ Lanmodulin-Functionalized Magnetic Nanoparticles as a Highly Selective Biosorbent for Recovery of Rare Earth Elements Quanhui Ye…Na Wei. Environ. Sci. Technol. 2023 MATERIALS Full-colour Jabuticaba-like nanostructures via the multiplex and orthogonal self-assembly of protein-conjugated quantum dots with engineered biofilms Zhengtao Deng…Timothy K Lu. Mater Horiz. 2023 Feb 14. MATERIALS Display of multiple proteins on engineered canine parvovirus-like particles expressed in cultured silkworm cells and silkworm larvae Jian Xu…Enoch Y. Park. Front. Bioeng. Biotechnol., 16 February 2023 IMMUN A Moonlighting Superpositively Charged SpyCatcher Tingting Yang…Wen-Bin Zhang. CCS Chemistry 15 Feb 2023 Carbohydrate fatty acid monosulphate: oil-in-water adjuvant enhances SARS-CoV-2 RBD nanoparticle-induced immunogenicity and protection in mice Etsuro Nanishi…Ofer Levy. npj Vaccines volume 8, Article number: 18 (2023) IMMUN Engineered Norovirus-Derived Nanoparticles as a Plug-and-Play Cancer Vaccine Platform Peng Zheng…Yanbing Ma. ACS Nano 2023 IMMUN MATERIALS Lactate oxidase/vSIRPα conjugates efficiently consume tumor-produced lactates and locally produce tumor- necrotic H2O2 to suppress tumor growth Yujin Kang…Sebyung Kang. Int J Biol Macromol 2023 Feb 7;123577. ENZ CD4 binding site immunogens elicit heterologous anti–HIV-1 neutralizing antibodies in transgenic and wild-type animals Harry B. Gristick…Pamela J. Bjorkman. Science Immunology 10 Feb 2023 IMMUN A bacterial outer membrane vesicle-based click vaccine elicits potent immune response against Staphylococcus aureus in mice Jingjing Sun…Jian-dong Huang. Front. Immunol., 19 January 2023 IMMUN Universal Design of Luciferase Fusion Proteins for Epigenetic Modifications Detection Based on Bioluminescence Resonance Energy Transfer Takamichi Miyata…Wataru Yoshida. Anal. Chem. Feb 2023 ENZ Orchestrating Chemical and Physical Cross-Linking in Protein Hydrogels to Regulate Embryonic Stem Cell Growth Tingting Yang…Wen-Bin Zhang. ACS Macro Lett. 2023, 12, 269–273 MATERIALS Isopeptide Bonding In Planta Allows Functionalization of Elongated Flexuous Proteinaceous Viral Nanoparticles, including Non- Viable Constructs by Other Means Daniel A. Truchado…Fernando Ponz. Viruses 2023, 15(2), 375 MATERIALS Cell-Free Dot Blot: an Ultra-Low-Cost and Practical Immunoassay Platform for Detection of Anti-SARS-CoV-2 Antibodies in Human and Animal Sera Masoud Norouzi…Keith Pardee. Microbiology Spectrum 2023 Feb IMMUN 750 UNIVERSAL RECEPTOR IMMUNE CELL THERAPY Patent WO/2023/004461 Publication Date 02.02.2023 PRESCIENT THERAPEUTICS LTD [AU]/[AU] IMMUN ALPHA-HEMOLYSIN VARIANTS FORMING NARROW CHANNEL PORES AND USES THEREOF Patent WO/2023/001784 Publication Date 26.01.2023 ROCHE DIAGNOSTICS GMBH [DE]/[DE] (DE) ESCRT recruitment to mRNA-encoded SARS-CoV-2 spike induces virus-like particles and enhanced antibody responses. Hoffmann MAG…Bjorkman PJ. bioRxiv. 2022 Dec 27:2022.12.26.521940 IMMUN Investigating the effects of cyclic topology on the performance of a plastic degrading enzyme for polyethylene terephthalate degradation Heather C. Hayes & Louis Y. P. Luk. Scientific Reports 13:1267 (2023) ENZ A Multivalent and Thermostable Nanobody Neutralizing SARS-CoV-2 Omicron (B.1.1.529) Lu Y…Chen Z. Int J Nanomed 19 January 2023 Volume 2023:18 Pages 353—367 IMMUN First-in-human use of a modular capsid virus-like vaccine platform: an open-label, non-randomised, phase 1 clinical trial of the SARS-CoV-2 vaccine ABNCoV2 Merel J Smit…Rolf Fendel, PhD. Lancet Microbe January 18, 2023 IMMUN Development of a Versatile Method to Construct Direct Electron Transfer-Type Enzyme Complexes Employing SpyCatcher/SpyTag System by Takumi Yanase…Wakako Tsugawa. Int. J. Mol. Sci. 2023, 24(3), 1837 ENZ Laccase-mediated formation of hydrogels based on silk-elastin-like protein polymers with ultra-high molecular weight S. Wang…W. Bai. International Journal of Biological Macromolecules 11 January 2023, 123239 ENZ MATERIALS Protein superglue inspired in-situ one-step site-specific immobilization of beta2-adrenoceptor and its application in bioactive compound screening from Cortex Magnoliae Officinalis Y Xue…X Zhao. Journal of Chromatography A, 8 Feb 2023, 1690, 463780 Improved Analysis of Cross-Linking Mass Spectrometry Data with Kojak 2.0, Advanced by Integration into the Trans-Proteomic Pipeline Michael R. Hoopmann…Robert L. Moritz. J. Proteome Res. 2023, Jan Effect of SpyTag/SpyCatcher cyclization on reactivation of covalently immobilized biocatalysts R Li…H Luo. Process Biochemistry 126, March 2023, Pages 87-97 ENZ MATERIALS Novel bovine viral diarrhea virus (BVDV) virus-like particle vaccine candidates presenting the E2 protein using the SpyTag/SpyCatcher system induce a robust neutralizing antibody response in mice Miki Katsura…Kaoru Takeuchi. Archives of Virology volume 168: 49 (2023) IMMUN A general strategy for protein affinity-ligand oriented-immobilization and screening for bioactive compounds Yi Y, Ying G. Journal of Chromatography B 2023 Jan MATERIALS Myddosome clustering in IL-1 receptor signaling regulates the formation of an NF-kB activating signalosome Fakun Cao…Marcus J. Taylor. bioRxiv Jan 2023 Comprehensive approach to study branched ubiquitin chains reveals roles for K48-K63 branches in VCP/p97-related processes Sven M. Lange…Yogesh Kulathu. bioRxiv Jan 2023 Lactate oxidase/catalase-displaying nanoparticles efficiently consume lactate in the tumor microenvironment to effectively suppress tumor growth Hyukjun Choi…Sebyung Kang. Journal of Nanobiotechnology 21, 5 (2023) ENZ SARS-COV-2 ANTIGEN NANOPARTICLES AND USES THERE OF Patent WO/2022/271916 Publication Date 29.12.2022 BORRIELLO, Francesco et al. IMMUN SELF-ASSEMBLING CIRCULAR TANDEM REPEAT PROTEINS WITH INCREASED STABILITY US patent Application Number 17624208 Publication Date 29.12.2022 Inventors Barry L. Stoddard et al. MATERIALS Construction of cell factory through combinatorial metabolic engineering for efficient production of itaconic acid Jiao Feng…Kequan Chen. Microbial Cell Factories volume 21, 275 (2022) ENZ Construction of Bi-Enzyme Self-Assembly Clusters Based on SpyCatcher/SpyTag for the Efficient Biosynthesis of (R)-Ethyl 2- hydroxy-4-phenylbutyrate Jinmei Wang…Zhimin Ou. Biomolecules 2023, 13(1), 91 ENZ Engineering a minimal SilkCatcher/Tag pair compatible with SpyCatcher/Tag pair for the production of native-sized spider silk Ruxia Fan…Sesilja Aranko. bioRxiv Dec 2022 MATERIALS SpyDisplay: A Versatile Phage Display Selection System using SpyTag/SpyCatcher Technology Sarah-Jane Kellmann…Francisco Ylera. bioRxiv Dec 2022 IMMUN Normalizing the Immune Macroenvironment via Debulking Surgery to Strengthen Tumor Nanovaccine Efficacy and Eliminate Metastasis Nana Ma…Xiao Zhao. ACS Nano 2022 Dec IMMUN Spy&IAC enables specific capture of SpyTagged proteins for rapid assembly of plug-and-display nanoparticle vaccines Yilan Chen…Gaiping Zhang. International Journal of Biological Macromolecules 226, 31 January 2023, Pages 240-253 IMMUN Stapled NRPS enhances the production of valinomycin in Escherichia coli Shuhui Huang…Jian Li. Biotech and Bioeng. 12 December 2022 ENZ 725 Stimulation of the immune system by a tumor antigen bearing adenovirus-inspired VLP allows the control of melanoma growth S Besson…P Fender. Mol Therapy 2022 Dec IMMUN Improvement of Modular Protein Display Efficiency in SpyTag-Implemented Norovirus-like Particles Jirayu Boonyakida… Enoch Y. Park. Biomacromolecules December 7, 2022 IMMUN PROTEIN PARTICLES COMPRISING A DIPHTHERIA TOXIN CROSS REACTING MATERIAL (CRM) AMINO ACID SEQUENCE AND USES THEREOF Patent US No.20220378894 Publication Date 01.12.2022 Inventors Bernd REHM et al. IMMUN Construction and Functionalization of a Clathrin Assembly for a Targeted Protein Delivery Hong-Sik Kim…Hak-Sung Kim. Small 2022 Dec Plug-and-Display Photo-Switchable Systems on Plant Virus Nanoparticles Louisa Kauth…Christina Dickmeis. BioTech Nov 2022 MATERIALS Ultra-lightweight living structural material for enhanced stiffness and environmental sensing Heechul Park…Tim Lu. Materials Today Bio. 2022 Nov and bioRxiv MATERIALS VIRUSES WITH MODIFIED CAPSID PROTEINS Patent United States of America Application Number 17755447 Publication Date 24.11.2022 Applicants SpyBiotech Limited IMMUN RESURFACED DENGUE VIRUS AND ZIKI VIRUS GLYCOPROTEIN E DIII VARIANTS AND USES THEREOF Patent United States Number 17621513 Applicants Jonathan R. Lai et al. Nov 2022 IMMUN A modular and self-adjuvanted multivalent vaccine platform based on porcine circovirus virus-like nanoparticles Ze-Hui Liu…Fang He. Journal of Nanobiotechnology volume 20, Article number: 493 (2022) IMMUN DNA segment capture by Smc5/6 holo-complexes Michael Taschner…Stephan Gruber. bioRxiv 2022 Oct Lipid nanodisc scaffold and size alters the structure of a pentameric ligand-gated ion channel Vikram Dalal…Wayland W. L. Cheng. bioRxiv 2022 Nov The Ribosome Maturation Factor Rea1 utilizes nucleotide independent and ATP hydrolysis driven Linker remodelling for the removal of ribosome assembly factors Johan Busselez…Helgo Schmidt. bioRxiv 2022 Nov Fiber manipulation and post-assembly nanobody conjugation for adenoviral vector retargeting through SpyTag-SpyCatcher protein ligation Maryam Kadkhodazadeh…Arash Arashkia. Front Mol Biosci Nov 2022 IMMUN A multivalent antibody assembled from different building blocks using tag/catcher systems: A case study Christof Schindler…Thomas Langer. Protein Engineering, Design and Selection, 14 November 2022 MATERIALS Two-component vaccine consisting of virus-like particles displaying hepatitis C virus envelope protein 2 oligomers Jannick Prentoe…Adam F. Sander. npj Vaccines volume 7, Article number: 148 (2022) IMMUN Heavy-chain CDR3-engineered B cells facilitate in vivo evaluation of HIV-1 vaccine candidates Wenhui He…Michael Farzan. bioRxiv 2022 Nov IMMUN Orthogonal protein decoration of DNA nanostructures based on SpyCatcher-SpyTag interaction Sandra Kröll…Christof M. Niemeyer. Chem Comm 2022 Nov ENZ MATERIALS Adenovirus-Inspired Virus-like-Particles Displaying Melanoma Tumor Antigen Specifically Target Human DC Subsets and Trigger Antigen-Specific Immune Responses Solène Besson…Pascal Fender. Biomedicines 2022 Nov IMMUN Non-Neutralizing Epitopes Shade Neutralizing Epitopes against Omicron in a Multiple Epitope-Based Vaccine Hua-Rui Gong…Jian-Dong Huang. ACS Infect. Dis. 2022 Nov IMMUN ADENOVIRAL VECTORS AND VACCINES THEREOF Patent WO/2022/234276 Publication Date 10.11.2022 SPYBIOTECH LIMITED IMMUN Spatial organization of pathway enzymes via self-assembly to improve 2′-fucosyllactose biosynthesis in engineered Escherichia coli Roulin Chen…Wanmeng Mu. Biotech and Bioeng, 2022 Nov ENZ Antibody-mediated delivery of CRISPR-Cas9 ribonucleoproteins in human cells Stephanie Ubiparipovic…Romain Rouet. Protein Engineering, Design and Selection. Nov 2022 Directed assembly of genetically engineered eukaryotic cells into living functional materials via ultrahigh-affinity protein interactions Qikun Yi…Fei Sun. Science Advances, Nov 2022 MATERIALS PREFUSION-STABILIZED LASSA VIRUS GLYCOPROTEIN COMPLEX AND ITS USE Patent WO/2022/232648 Publication Date 03.11.2022 Inventors KWONG, Peter et al. IMMUN Improving the Synthesis Efficiency of Amino Acids Such as L-Lysine by Assembling Artificial Cellulosome Elements Dockerin Protein In Vivo Nan Li…Ruiming Wang. Fermentation 2022 Oct ENZ 700 Organizing Enzymes on Self-Assembled Protein Cages for Cascade Reactions Wei Kang…Chuang Xue. Ang Chem 2022 Oct ENZ Intranasal virus-particle mimicking vaccine enhances SARS-CoV-2 clearance in the Syrian hamster model Devanshi R Patel…Troy C Sutton. bioRxiv 2022 Oct IMMUN COMBINATION OF PEPTIDE LINKERS FOR PROTEIN COVALENT SELF-ASSEMBLY USING SPONTANEOUS ISOPEPTIDE BOND Patent WO/2022/222700 Publication Date 27.10.2022 ZHANG, Hui et al. Improving cross-protection against influenza virus in mice using a nanoparticle vaccine of mini-HA Hechao Zhu…Ping Qian. Vaccine Oct 2022 IMMUN Splicing factor BUD31 promotes ovarian cancer progression through sustaining the expression of anti- apoptotic BCL2L12 Zixiang Wang…Zhaojian Liu. Nature Communications 13, 6246 (2022) Plug-and-Display Photo-Switchable Systems on Plant Virus Nanoparticles Louisa Kauth…Christina Dickmeis. BioTech Oct 2022 MATERIALS The synergism of lytic polysaccharide monooxygenases with lichenase and their co-immobilization on silica nanospheres for green conversion of lichen biomass Lixi Cai…Guangya Zhang. Front. Nutr., 19 October 2022 ENZ NOVEL UNIVERSAL VACCINE PRESENTING SYSTEM Patent WO/2022/218997 Publication Date 20.10.2022 FENDER, Pascal et al. IMMUN Macromolecular assembly of bioluminescent protein nanoparticles for enhanced imaging Enya Li…Szu-Wen Wang. Materials Today, Oct 2022 IMAGING SpyStapler-mediated assembly of nanoparticle vaccines Songzi Kou…Fei Sun. Nano Research (2022) IMMUN Engineering synthetic microbial consortium for cadaverine biosynthesis from glycerol Simin Liu…Lei Zhang. Biotechnology Letters (2022) Oct ENZ Enhanced thermal stability of the β-galactosidase BgaB from Bacillus circulans by cyclization mediated via SpyTag/SpyCatcher interaction and its use in galacto-oligosaccharides synthesis Mingming Han… Yongqin Ni. Int Journal Biol Macromolecules Oct 2022 ENZ SpyPhage: A Cell-Free TXTL Platform for Rapid Engineering of Targeted Phage Therapies Sahan B. W. Liyanagedera…Vishwesh Kulkarni. ACS Syn Bio Oct 2022 MATERIALS Site-Specific and Tunable Co-immobilization of Proteins onto Magnetic Nanoparticles via Spy Chemistry Quanhui Ye…Na Wei. ACS Appl. Bio Mater. 2022 MATERIALS Heterologous Expression of Thermotolerant α-Glucosidase in Bacillus subtilis 168 and Improving Its Thermal Stability by Constructing Cyclized Proteins Zhi Wang…Zhiming Rao. Fermentation 2022, 8(10), 498 ENZ MULTIVALENT PROTEINS AND SCREENING METHODS WO/2022/200804 Publication Date 29.09.2022 LILIUMX Inventors SCHEU, Arne, KHAIRIL ANUAR, Irsyad, LIM, Sheryl Ying Ting MATERIALS Display of Streptococcus iniae α-Enolase on the Surface of Virus-Like Particles (VLPs) of Nervous Necrosis Virus (NNV) Using SpyTag/SpyCatcher Jeong In Yang & Ki Hong Kim. Marine Biotechnology (2022) IMMUN Cryo-EM reveals a mechanism of USP1 inhibition through a cryptic binding site Martin L. Rennie…Helen Walden. Science Advances 28 Sep 2022 A de novo matrix for macroscopic living materials from bacteria Sara Molinari…Caroline M. Ajo-Franklin. Nature Communications volume 13, Article number: 5544, 2022 Sept MATERIALS Mechanical Stabilization of a Bacterial Adhesion Complex Wenmao Huang…Jie Yan. J. Am. Chem. Soc. 2022, 144, 37, 16808–16818 Molecular engineering of a cryptic epitope in Spike RBD improves manufacturability and neutralizing breadth against SARS-CoV-2 variants Sergio A. Rodriguez-Aponte…J. Christopher Love. bioRxiv 2022 Sept IMMUN Directional immobilization of D-allulose 3-epimerase using SpyTag/SpyCatcher strategy as a robust biocatalyst for synthesizing D-allulose Xin Gao…Hui-Min Qin. Food Chem Sept 2022 ENZ Long-term retention of antigens in germinal centres is controlled by the spatial organisation of the follicular dendritic cell network Ana Martinez-Riano…Pavel Tolar, bioRxiv Sept 2022 IMMUN CD4-binding site immunogens elicit heterologous anti-HIV-1 neutralizing antibodies in transgenic and wildtype animals Harry B Gristick…Pamela J Bjorkman. bioRxiv Sept 2022 IMMUN Mechanical regulation of Titin N2B-us conformation and its binding to FHL2 Yuze Sun…Jie Yan. bioRxiv Sept 2022 MATERIALS 675 AN ARTIFICIAL PROTEIN-CAGE DECORATED WITH PARTICULAR MOLECULES ON THE EXTERIOR Patent WO/2022/182260 Publication Date 01.09.2022 HEDDLE, Jonathan et al. MATERIALS AN ARTIFICIAL PROTEIN-CAGE COMPRISING ENCAPSULATED THEREIN A GUEST CARGO Patent WO/2022/182262 Publication Date 01.09.2022 HEDDLE, Jonathan et al. MATERIALS ENDOTOXIN-FREE PRODUCTION OF RECOMBINANT SUBUNIT VACCINE COMPONENTS Patent WO/2022/175871 Publication Date 25.08.2022 INGENZA LTD [GB]/[GB] IMMUN Directing ricin-based immunotoxins with targeting affibodies and KDEL signal peptide to cancer cells effectively induces apoptosis and tumor suppression Seong Guk Park…Sebyung Kang. Journal of Nanobiotechnology volume 20, Article number: 387 (2022) Tailoring the Tag/Catcher System by Integrating Covalent Bonds and Noncovalent Interactions for Highly Efficient Protein Self-Assembly Yao Chen…Ling Jiang. Biomacromolecules 2022 MATERIALS ENZ Intracellular Assembly of Interacting Enzymes Yields Highly-Active Nanoparticles for Flow Biocatalysis Patrick Bitterwolf, Ahmed E. Zoheir, Julian Hertel, Sandra Kröll, Kersten S. Rabe, Christof M. Niemeyer. Chem Eur J Aug 2022 MATERIALS ENZ ADHESIVE PROTEIN Patent Application Number 17628132 Publication Date 18.08.2022 Katsutoshi Hori MATERIALS Single-step purification of functionalized protein nanostructures using multimodal chromatography Daniel L. Winter…Dominic J. Glover. J Bionanotechnology 2023 and bioRxiv MATERIALS Passive immunization with equine RBD-specific Fab protects K18-hACE2-mice against Alpha or Beta variants of SARS-CoV-2 Mariette Barbier…F. Heath Damron. Front Imm Aug 2022 IMMUN RBD-VLP Vaccines Adjuvanted with Alum or SWE Protect K18-hACE2 Mice against SARS-CoV-2 VOC Challenge Ting Y. Wong…F. Heath Damron. mSphere Aug 2022 IMMUN SPECIFIC SELECTION OF IMMUNE CELLS USING VERSATILE DISPLAY SCAFFOLDS US patent Application Number 17614711 Publication Date 04.08.2022 Scott Eugene LINDNER et al. IMMUN MODIFIED VIRAL PARTICLES AND USES THEREOF Patent Application Number 17612669 Publication Date 04.08.2022 Regeneron Pharmaceuticals, Inc. Valence-controlled protein conjugation on nanoparticles via re-arrangeable multivalent interactions of tandem repeat protein chains Hyeongjoo Choi, Yongwon Jung. Chem Sci Aug 2020 MATERIALS A "Plug-and-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-binding Domain Rang Feng…Hai-Bo Li. J Vis Exp 2022 Jul 25 IMMUN Protein Cages Engineered for Interaction-Driven Selective Encapsulation of Biomolecules Yeolin Lee…Yongwon Jung. ACS Appl. Mater. Interfaces Aug 2022 MATERIALS Interactions between mTORC2 core subunits Rictor and mSin1 dictate selective and context-dependent phosphorylation of substrate kinases SGK1 and Akt. Zanlin Yu…David Pearce. J Biol Chem Aug 2022 Bacterial biofilm functionalization through Bap amyloid engineering Leticia Matilla-Cuenca…Jaione Valle. npj Biofilms and Microbiomes 8:62 (2022) MATERIALS Convection Driven Ultrarapid Protein Detection via Nanobody-Functionalized Organic Electrochemical Transistors Anil Koklu…Sahika Inal. Advanced Materials July 2022 MATERIALS Development of a synthetic nanoparticle vaccine presenting the HIV-1 envelope glycoprotein Phindile Ximba…Edward Rybicki. Nanotechnology 26 July 2022 IMMUN Nanocarriers based on bacterial membrane materials for cancer vaccine delivery Xiao Zhao…Guangjun Nie. Nature Protocols (2022) July IMMUN Mechanical regulation of talin through binding and history-dependent unfolding Narayan Dahal…Ionel Popa. Science Advances 15 Jul 2022 MATERIALS TRAIL & EGFR affibody dual-display on a protein nanoparticle synergistically suppresses tumor growth Heejin Jun…Eunhee Kim. J Control Release 2022 Jul 6 MATERIALS Self-Assembling Protein Surfaces for In Situ Capture of Cell-Free-Synthesized Proteins Ella Lucille Thornton…Lynne Regan. Front. Bioeng. Biotechnol., 07 July 2022 MATERIALS Neutralizing monoclonal antibodies elicited by mosaic RBD nanoparticles bind conserved sarbecovirus epitopes Chengcheng Fan…Pamela J. Bjorkman. bioRxiv Jun 2022 IMMUN Steric accessibility of the N-terminus improves the titer and quality of recombinant proteins secreted from Komagataella phaffii Neil C. Dalvie…Christopher Love. bioRxiv Jun 2022 and Microb Cell Fact Sept 2022 IMMUN 650 Mosaic RBD nanoparticles protect against challenge by diverse sarbecoviruses in animal models Alexander A. Cohen…Pamela J. Bjorkman.. Science 5 Jul 2022 IMMUN Liquid–Liquid Phase Separation and Assembly of Silk-like Proteins is Dependent on the Polymer Length Laura Lemetti…A. Sesilja Aranko. Biomacromolecules 2022 Jul MATERIALS METALLOENZYMES FOR BIOMOLECULAR RECOGNITION OF N-TERMINAL MODIFIED PEPTIDES Patent WO/2022/147334 ENCODIA, INC. [US]/[US] ENZ SEQUENCING ANTIBODY-GUIDED PCSK9-MIMICKING IMMUNOGENS LACKING 9-RESIDUE SEQUENCE OVERLAP WITH HUMAN PROTEINS Patent WO/2022/147373 Publication Date 07.07.2022 KWONG, Peter D. et al. IMMUN Sequentially co-immobilized PET and MHET hydrolases via Spy chemistry in calcium phosphate nanocrystals present high-performance PET degradation Kun Chen…Yan Sun. Journal of Hazardous Materials. Jul 2022 MATERIALS SpySwitch enables pH- or heat-responsive capture and release for plug-and-display nanoassembly. Vester SK, Rahikainen R, Khairil Anuar INA, Hills RA, Tan TK, Howarth M. Nat Commun. 2022 Jun 28;13(1):3714. IMMUN A modular dCas9-based recruitment platform for combinatorial epigenome editing Tessa Swain…Ryan Lister. bioRxiv Jul 2022 NUCLEIC ACID BINDING PROTEIN FORMULATIONS AND USES THEREOF Patent WO/2022/140795 Publication Date 30.06.2022 SPOTLIGHT THERAPEUTICS [US]/[US] Multienzyme Assembly on Caveolar Membranes In Cellulo Qixin Wei…Jiang Xia. ACS Catal. 2022, 12, 8372–8379 ENZ NUCLEIC ACID VACCINES Patent WO/2022/129547 Publication Date 23.06.2022 UNIVERSITY OF COPENHAGEN [DK]/[DK] ADAPTVAC APS [DK]/[DK] IMMUN UNIVERSAL BACTERIOPHAGE T4 NANOPARTICLE PLATFORM TO DESIGN MULTIPLEX SARS-COV-2 VACCINE CANDIDATES BY CRISPR ENGINEERING Patent WO/2022/130196 Publication Date 23.06.2022 RAO, Venigalla B. ZHU, Jingen IMMUN Freeze-Drying of a Capsid Virus-like Particle-Based Platform Allows Stable Storage of Vaccines at Ambient Temperature Kara-Lee Aves…Adam F. Sander. Pharmaceutics June 2022 IMMUN Two-Dimensional Protein Nanoarray as a Carrier of Sensing Elements for Gold-Based Immunosensing Systems Jingya Tang…Dian-Bing Wang. Anal. Chem. June 21, 2022 MATERIALS Induction of Broadly Cross-Reactive Antibody Responses to SARS-CoV-2 Variants by S1 Nanoparticle Vaccines Cong Sun…Yin-Feng Kang. J Virol Jun 2022 IMMUN Peptide-Inspired One-Step Synthesis of Surface-Functionalized Fe3O4 Magnetic Nanoparticles for Oriented Enzyme Immobilization and Biocatalytic Applications Yanhong Zhou…Guangya Zhang. ACS Appl. Nano Mater. Jun 2022 ENZ DNA passes through cohesin’s hinge as well as its Smc3-kleisin interface James E Collier and Kim A Nasmyth. bioRxiv May 2022 and eLife Sept 2022 Increased Thermal Stability of the Carbonic Anhydrase Enzyme Complex for the Efficient Reduction of CO2 through Cyclization and Polymerization by Peptide Bonding Habin Sun…Jeong Eun Hyeon. Process Biochemistry, 6 June 2022 ENZ A Powassan virus domain III nanoparticle immunogen elicits neutralizing and protective antibodies in mice Ryan J. Malonis…Jonathan R. Lai. PLoS Path Jun 2022 IMMUN ENGINEERED PROBIOTIC COMPOSITIONS AND USES THEREOF Patent WO/2022/119926 Publication Date 09.06.2022 CUBILLOS, Andres Fernando, COLLINS, James J. Quaternary structure independent folding of voltage-gated ion channel pore domain subunits Cristina Arrigoni…Daniel L. Minor Jr. Nat Struct Mol Biol, Jun 2022 RECONSTITUTION OF A SPLIT-HALOTAG VIA ORTHOGONAL TAG-BINDING DOMAINS WO/2022/115696 Publication Date 02.06.2022 HUANG, Bo et al. MULTIPLEXED PROFILING OF RNA AND DNA MODIFICATIONS Patent WO/2022/115608 Publication Date 02.06.2022 ALIDA BIOSCIENCES, INC. [US]/[US] CRISPR-mediated protein-tagging signal amplification systems for efficient transcriptional activation and repression in Saccharomyces cerevisiae Haotian Zhai…Jin Hou. Nuc Acid Res May 2022 LIGHT-CONTROLLED VIRAL TRANSDUCTION Patent WO/2022/106690, Publication Date 27.05.2022 HÖRNER, Maximilian; WEBER, Wilfried Programmable protein topology via SpyCatcher-SpyTag chemistry in one-pot cell-free expression system Qingning Ye…Yuan Lu. Protein Science 21 May 2022 625 A loosened gating mechanism of RIG-I leads to autoimmune disorders Yixuan Lei…Jie Zheng. Nucleic Acid Res May 2022 IMMUN MULTIVALENT CARRIERS AND RELATED VACCINE COMPOSITIONS Patent WO/2022/103891 Publication Date 19.05.2022 COHEN, Alexander, A.; BJORKMAN, Pamela, J. IMMUN SCAFFOLDED ANTIGENS AND ENGINEERED SARS-COV-2 RECEPTOR-BINDING DOMAIN (RBD) POLYPEPTIDES WO/2022/104265 Publication Date 19.05.2022 THE SCRIPPS RESEARCH INSTITUTE [US]/[US] EMMUNE, INC. [US]/[US] IMMUN Non-Covalently Associated Streptavidin Multi-Arm Nanohubs Exhibit Mechanical and Thermal Stability in Protein-Network Materials David S Knoff…Minkyu Kim. Biomacromolecules Sept 2022 and bioRxiv May 2022 MATERIALS A Capsid Virus-Like Particle-Based SARS-CoV-2 Vaccine Induces High Levels of Antibodies and Protects Rhesus Macaques. Ariane Volkmann…Paul Chaplin. Front. Immunol. 05 April 2022 IMMUN SARS-COV-2 RBD CONJUGATED NANOPARTICLE VACCINE WO/2022/088953 Publication Date 05.05.2022 SUN YAT-SEN UNIVERSITY IMMUN A Magnetosome-Based Platform for Flow Biocatalysis Esther Mittmann…Dirk Schüler. ACS Appl. Mater. Interfaces May 2022 MATERIALS Free energy landscape of two-state protein Acylphosphatase with large contact order revealed by force- dependent folding and unfolding dynamics Xuening Ma…Hu Chen. Phys Rev, Jul 2022 and arXiv Mar 2022 MATERIALS A bacteriophage-based, highly efficacious, needle and adjuvant-free, mucosal COVID-19 vaccine Jingen Zhu…Venigalla B. Rao. mBio Jul 2022 and bioRxiv Apr 2022 IMMUN BACTERIAL MICROCOMPARTMENT VIRUS-LIKE PARTICLES Patent WO/2022/086450 Publication Date 28.04.2022 TAN, Yong Quan et al. METHODS AND COMPOSITIONS FOR RAPIDLY REPLACING CARDIAC MYOSIN BINDING PROTEIN-C IN SARCOMERES Patent Application Number 17559865 Publication Date 21.04.2022 Samantha P. Harris MATERIALS Evolutionary balance between foldability and functionality of a glucose transporter Hyun-Kyu Choi…Tae-Young Yoon. Nature Chemical Biology (Apr 2022) MATERIALS Estimation of microtubule-generated forces using a DNA origami nanospring Ali Nick Maleki…Vladimir A. Volkov. bioRxiv Apr 2022 MATERIALS BTB-BCL6 dimers as building blocks for reversible drug-induced protein oligomerization Lena Nitsch…MikołajSłabicki. Cell Reports Methods Apr 2022 An engineered live biotherapeutic for the prevention of antibiotic-induced dysbiosis Andrés Cubillos-Ruiz…James J. Collins. Nature Biomedical Engineering 2022 ENZ POLYPEPTIDE TERMINAL BINDERS AND USES THEREOF WO/2022/072560 Publication Date 07.04.2022 ENCODIA, INC. [US]/[US] SEQUENCING Engineering functional membrane-membrane interface by InterSpy Hossein Moghimianavval…Taekjip Ha. Small 26 May 2022 and bioRxiv 2022 Mar IMAGING Mosaic RBD nanoparticles protect against multiple sarbecovirus challenges in animal models Alexander A. Cohen…Pamela J. Bjorkman. bioRxiv April 2022 IMMUN B12-induced reassembly of split photoreceptor protein enables photoresponsive hydrogels with tunable mechanics Zhongguang Yang…Fei Sun. Science Advances Apr 2022 MATERIALS From 4-arm star proteins to diverse stimuli-responsive molecular networks enabled by orthogonal genetically encoded click chemistries Hong Kiu Francis Fok…Fei Sun. Polymer Chemistry Apr 2022 MATERIALS Effect of SpyTag/SpyCatcher cyclization on stability and refolding of green fluorescent protein Haiyan Song…Hui Luo. Biotechnology Letters Mar 2022 IMAGING POLYPEPTIDE TAGGED NUCLEOTIDES AND USE THEREOF IN NUCLEIC ACID SEQUENCING BY NANOPORE DETECTION Patent Application Number 17201834 Publication Date 24.03.2022 Roche Sequencing Solutions, Inc. SEQUENCING SpyChIP identifies cell type-specific transcription factor occupancy from complex tissues Siqian Feng, Richard S Mann. PNAS June 13, 2022 and bioRxiv Mar 2022 In vitro refolding of the structural protein VP1 of parvovirus B19 produces virus-like particles with functional VP1 unique region. Soto-Valerio, I.A….Bustos-Jaimes, I. Virology (2022) March IMMUN Comparison of Bacterial Expression Systems Based on Potato Virus Y-like Particles for Vaccine Generation by Anete Ogrina…Andris Zeltins. Vaccines 2022, 10(4), 485 IMMUN 301-600 publications here. 1-300 publications here.
It's the oldest question of all, George.  Who can spy on the spies?  ― John le Carré