ADDRESS Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD UK
CONTACT e: t: 01223 334176
2024 Mark Howarth. All rights reserved.
Papers and Patents using Spy technology Many of these publications don’t come up from a Pubmed search or citation search, so it should be useful to bring them all together and hyperlinked (now >1,000 publications). Patents may be omitted if there is a journal paper covering very similar work. Ones with particular relevance to IMMUNOLOGY, MATERIALS (polymers or mechanobiology), IMAGING , SEQUENCING or ENZYMES are marked. For an overview, please see our open-access review “Power to the Protein” from 2020. SpyBank is a database of >1,000 Tag/Catcher aa sequences to download (Excel file), from publications below. SpyBank includes includes expression organism, Tag/Catcher location (N-terminal, internal, C-terminal) and annotated linkers, as a reference for groups creating their own fusions. SpyBank itself is described in: “Insider information on successful covalent protein coupling with help from SpyBank. Keeble AH, Howarth M. Meth Enz 2019 PDF”. (See note on codon usage at Reagents page). Most recent below. Publications 301-600 here. Publications 1-300 here. VCP/p97-associated proteins are binders and debranching enzymes of K48–K63-branched ubiquitin chains Sven M. Lange…Yogesh Kulathu. Nature Structural & Molecular Biology (2024) ENZ Orchestrated Codelivery of Peptide Antigen and Adjuvant to Antigen-Presenting Cells by Using an Engineered Chimeric Peptide Enhances Antitumor T-Cell Immunity Haifeng Pan…Ningshao Xia. Cancer Immunol Res (2024) 12 (7): 905–920 IMMUN Induction of neutralising antibodies against conserved rhinovirus capsid protein VP4 depends on presenting VP4 in a virus-like conformation James T. Kelly…Tobias J. Tuthill. bioRxiv 2024 Jul IMMUN Genetically Decorating Microbial Surface Curli Fiber with Biocatalytic Properties Qingxia Wang…Jinyong Yan. ACS Sustainable Chem. Eng. 2024, Jul MATERIALS ENZ COMPOSITIONS AND METHODS FOR CONTROLLED MRNA TRANSLATION AND STABILITY Patent WO/2024/137990 Publication Date 27.06.2024 Inventors MARZILLI, Alexander Michael, NGO, John Tuan Genetically Programmed Temperature-Responsive Barnacle-Derived Protein with an Enhanced Adhesion Ability Yuanxing Shi…Jinyong Yan. ACS Appl. Bio Mater. 2024 MATERIALS Engineering Mesoscale T Cell Receptor Clustering by Plug-and-Play Nanotools M Florencia Sánchez…Robert Tampé. Adv Mater 2024 Jun 25:e2310407. IMMUN PARTICLE DELIVERY SYSTEMS Patent WO/2024/129976 Publication Date 20.06.2024 Applicants SCRIBE THERAPEUTICS INC. [US]/[US] ANTIGEN-BINDING MOLECULES THAT BIND TO AAV PARTICLES AND USES WO/2024/130175 Publication Date 20.06.2024 Applicants REGENERON PHARMACEUTICALS, INC. [US]/[US] IMMUN Oriented immobilization of nanobodies using SpyCatcher/SpyTag significantly enhances the capacity of affinity chromatography Yuxiang Zhang…Yongdong Liu. J Chromatography A 2024 Jun Development of the IMP biosensor for rapid and stable analysis of IMP concentrations in fermentation broth Shibo Jiang…Suiping Zheng. Biotech J 11 June 2024 ENZ BROADLY NEUTRALIZING ANTIBODY-TEMPLATED DIVALENT IMMUNOGEN VACCINES WO/2024/123708 Publication Date 13.06.2024 UNIVERSITY OF HAWAII [US]/[US] Inventors MACPHERSON, Iain et al. IMMUN AN ARTIFICIAL PROTEIN-CAGE COMPRISING ENCAPSULATED THEREIN A GUEST CARGO United States of America Application Number 18547242 Publication Date 06.06.2024 Applicants UNIWERSYTET JAGIELLONSKI Inventors Jonathan HEDDLE et al. MATERIALS COMPOSITIONS AND METHODS FOR FIBROBLAST GROWTH FACTOR RECEPTOR 3-MEDIATED DELIVERY TO ASTROCYTES United States of America Application Number 18509030 Publication Date 30.05.2024 Regeneron Pharmaceuticals, Inc. Dual role of the peptide- loading complex as proofreader and limiter of MHC- I presentation Jamina Brunnberga…Robert Tampé. PNAS 2024 IMMUN Cu(II)/THPTA-Mediated Thiazolidine Deprotection for Living Phages and Cell Surfaces Labeling Chengyun Ma…Xiaobao Bi. ChemRxiv 07 June 2024 MATERIALS Development of an improved blood-stage malaria vaccine targeting the essential RH5-CyRPA-RIPR invasion complex Barnabas G. Williams…Simon J. Draper. Nature Communications volume 15, Article number: 4857 (2024) IMMUN AlbuCatcher for Long-Acting Therapeutics Ji Hyun Rho…Inchan Kwon. ACS Omega 2024, 9, 21, 22990–23000 Nanoparticle display of neuraminidase elicits enhanced antibody responses and protection against influenza A virus challenge M N Pascha…C A M de Haan. NPJ Vaccines. 2024 May 31;9(1):97. IMMUN Single immunization of non-adjuvanted recombinant TTFC-mi3 nanoparticle vaccine elicited a rapid and potent protective immunity against tetanus Qinggang He…Yi Zhang. Vaccine 2024 May IMMUN Microgels with Immobilized Glycosyltransferases for Enzymatic Glycan Synthesis Isabel Katja Sommerfeld…Andrij Pich. Biomacromolecules 2024 May MATERIALS ENZ Enhancing the Stress Resistance of Nitrile Hydratase from Rhodococcus ruber via SpyTag/SpyCatcher-mediated α- and β- subunits ligation Miaomiao Wang, Huimin Yu. Research Square 2024 May ENZ Design of a genetically programmable and customizable protein scaffolding system for the hierarchical assembly of robust, functional macroscale materials Ruijie Zhang…Claudia Schmidt- Dannert. bioRxiv 2024 May MATERIALS 1025 Mosaic and cocktail capsid-virus-like particle vaccines for induction of antibodies against the EPCR-binding CIDRα1 domain of PfEMP1 Ilary Riedmiller…Louise Turner. bioRxiv 2024 April IMMUN Genetically modifying the protein matrix of macroscopic living materials to control their structure and rheological properties Esther M. Jimenez…Caroline M. Ajo-Franklin. bioRxiv 2024 May MATERIALS Development and Characterization of Recombinant ADP-Ribose Binding Reagents that Allow Simultaneous Detection of Mono and Poly ADP-Ribose Shu-Ping Chiu…W. Lee Kraus. bioRxiv 2024 May Preserving ester-linked modifications reveals glutamate and aspartate mono-ADP-ribosylation by PARP1 and its reversal by PARG Edoardo José Longarini & Ivan Matić. Nature Communications volume 15, Article number: 4239 (2024) ENZ TALIN-BASED MECHANOSENSORY HYDROGEL Patent WO/2024/100399 Publication Date 16.05.2024 Applicants UNIVERSITY OF KENT [GB]/[GB] Inventors HISCOCK, Jennifer, GOULT, Ben MATERIALS Reengineering of an Artificial Protein Cage for Efficient Packaging of Active Enzymes Yusuke Azuma…Jonathan G. Heddle. Small 2024 May MATERIALS Comparative analysis of folding and unfolding dynamics and free-energy landscapes in homologous cold shock proteins with variable thermal stabilities Zhenyong Xue…Hu Chen. Phys. Rev. Research 6, 023170 – Published 14 May 2024 Engineering Pickering Emulsion with Cell Surface-Displayed Esterase Est7 for Specific and Efficient Synthesis of EPA Monoglyceride Kunpeng Gao…Xiangzhao Mao. ACS Sustainable Chem. Eng. 2024 MATERIALS ENZ Mosaic RBD nanoparticle elicits immunodominant antibody responses across sarbecoviruses Chuanyu Liu…George F. Gao. Cell Reports 2024 May IMMUN MODIFIED COVALENTLY-LINKED PILI AND RECOMBINANT BACTERIA COMPRISING THE SAME Patent WO/2024/092769 Publication Date 10.05.2024 Applicants SHENZHEN INSTITUTE OF ADVANCED TECHNOLOGY Inventors ZHONG, Chao et al. MATERIALS UNIVERSAL ADJUVANT FOR NASAL, ORAL, AND INTRAMUSCULAR DELIVERY OF VACCINES Patent United States of America Publication Date 02.05.2024 Applicants Design-Zyme LLC IMMUN SELF-ADJUVANTING MULTI-PROTEIN COMPLEXES FOR MODULAR VACCINE PRODUCTION Patent WO/2024/097817 Publication Date 10.05.2024 Applicants DESIGN-ZYME LLC [US]/[US] IMMUN Customizable Click Biochemistry Strategy for the Design and Preparation of Glucagon-like Peptide-1 Conjugates and Coagonists Yunchun Zheng…Xiaofeng Yang. Bioconjug Chem. 2024 May 3. Nanobody in a Double “Y”-Shaped Assembly: A Promising Candidate for Lateral Flow Immunoassays Yumeng Xiao…Lichuan Gu. Anal. Chem. 2024 IMMUN Ligand-dependent folding and unfolding dynamics and free energy landscapes of acylphosphatase Li Yuan…Hu Chen. Soft Matter 2024 Apr ENZ Construction of Switch Modules for CAR-T Cell Treatment Using a Site-Specific Conjugation System Tuersunayi Abudureheman…Kai-Ming Chen. Bioconjugate Chem. 2024 IMMUN SpyTag Peptide with Alkoxyl Aspartic Acids for pH-Dependent Activation of the SpyCatcher/Tag System Sonji Che…Koki Makabe. Bioconjugate Chem. 2024 Catalytic Synthesis of (S)-CHBE by Directional Coupling and Immobilization of Carbonyl Reductase and Glucose Dehydrogenase Yadong Wang…Fenghuan Wang. Biomolecules 2024, 14(4), 504 ENZ BUOYANCY ACTIVATED CELL SORTING AND SCALABLE PROTEIN PURIFICATION WITH ENGINEERED GAS VESICLES Patent WO/2024/086802 Publication Date 25.04.2024 LU, Jiaozhi et al. MATERIALS POLYPEPTIDE FUSIONS OR CONJUGATES FOR GENE EDITING Patent WO/2024/086596 Publication Date 25.04.2024 4M GENOMICS INC. ENZ SYNTHETIC INTERMEDIATES FOR UNIVERSAL CHIMERIC ANTIGEN RECEPTOR IMMUNE CELL THERAPIES Patent WO/2024/081824 Publication Date 18.04.2024 Applicants UNIVERSITY OF WASHINGTON [US]/[US] Inventors SELLERS, Drew et al. IMMUN BIOCOMPATIBLE QUANTUM DOT-POLYMER COMPOSITE AND DIAGNOSTIC KIT USING SAME Patent 18276829 Publication Date 11.04.2024 Applicants UNIST Inventors Jongnam Park et al. MATERIALS Detoxifying Cyanides Using Cyanase Enzyme Complexes Composed of Carbonic Anhydrase via Irreversible Covalent Bonds Habin Sun…Jeong Eun Hyeon. J. Agric. Food Chem. 2024, 72, 10, 5318–5324 ENZ Antigen-functionalized turnip mosaic virus nanoparticles increase antibody sensing in saliva. A case study with SARS-CoV-2 RBD Carlos Medrano-Arranz…Daniel A. Truchado. Diagnostic Microbiology and Infectious Disease Volume 109, Issue 3, July 2024, 116298 IMMUN METHODS AND COMPOSITIONS FOR T CELL DIFFERENTIATION Patent WO/2024/076499 Publication Date 11.04.2024 Inventors DALEY, George Q. et al. IMMUN 1000 SYSTEMS, FORMULATIONS AND METHODS FOR GENERATING UNIVERSAL PEPTIDE/MHC COMPLEXES WITH ENGINEERED DISULFIDE CONNECTING THE HEAVY AND LIGHT CHAINS Patent WO/2024/072958 Publication Date 04.04.2024 Inventors SGOURAKIS, Nikolaos et al. IMMUN DIRECTED IN VIVO AFFINITY MATURATION OF ANTIBODIES Patent WO/2024/073744 Publication Date 04.04.2024 Inventors FARZAN, Michael R. et al. IMMUN Overcoming Protein Orientation Mismatch Enables Efficient Nanoscale Light-Driven ATP Production Andrea Marco Amati…Christoph von Ballmoos. ACS Synth. Biol. April 3, 2024 MATERIALS Precision Engineering the Co-Immobilization of Enzymes for Cascade Biocatalysis Zhiyuan Luo…Roger A Sheldon. Angew Chem Int Ed Engl 2024 Mar 31:e202403539. ENZ Proofreading and single-molecule sensitivity in T-cell receptor signaling by condensate nucleation William L White…Hao Yuan Kueh. bioRxiv 2024 Mar IMMUN AN ENZYMATIC SYSTEM FOR PRECISE CELL TARGETING Patent WO/2024/064818 Publication Date 28.03.2024 HOLDEN, Rebecca ENZ AFFINITY CAPTURING AND DIRECTLY DETERMINING STRUCTURES OF PROTEINS AND OTHER MATERIALS ON SUPERPARAMAGNETIC BEADS BY CRYO-ELECTRON MICROSCOPY SINGLE-PARTICLE ANALYSIS WO/2024/064112 Publication Date 28.03.2024 ARIMURA, Yasuhiro et al. MATERIALS Construction of a versatile fusion protein for targeted therapy and immunotherapy Xiu-Song Huang…Cai-Wen Duan. Protein Science 19 March 2024 IMMUN Rational Design of Spontaneous Self-Cyclization Enzymes In Vivo and In Vitro with Improved Thermal Tolerance and Activity Bin Wei…Hao Liang. ACS Catal. 2024, 14, 5469–5480 March 28, 2024 ENZ Intranasal delivery of Salmonella OMVs decorated with Chlamydia trachomatis antigens induces specific local and systemic immune responses Dung T. Huynh…Joen Luirink. Human Vaccines & Immunotherapeutics Volume 20, 2024 IMMUN SpyMask enables combinatorial assembly of bispecific binders Claudia L. Driscoll…Mark R. Howarth. Nature Communications volume 15, Article number: 2403 (2024) IMMUN Molecular density-accelerated binding-site maturation underlies CENP-T-dependent kinetochore assembly Ekaterina V. Tarasovetc…Ekaterina L. Grishchuk. bioRxiv 2024 Rational structure-guided design of a blood stage malaria vaccine immunogen presenting a single epitope from PfRH5 Thomas E. Harrison…Matthew K. Higgins. bioRxiv 2024 IMMUN Designed mosaic nanoparticles enhance cross-reactive immune responses in mice Eric Wang…Arup K. Chakraborty. bioRxiv 2024 IMMUN Architecture and flexibility of native kinetochores revealed by structural studies utilizing a thermophilic yeast Daniel J. Barrero…Sue Biggins. bioRxiv 2024 METHODS OF PRODUCING EXTRACELLULAR VESICLES Patent Application Number 18246304 Publication Date 14.03.2024 Applicants LONZA SALES AG Nanoparticle vaccines based on the receptor binding domain of porcine deltacoronavirus elicit robust protective immune responses in mice Yuanhong Wang…Lingxue Yu. Front. Immunol., 14 March 2024 IMMUN A cVLP-Based Vaccine Displaying Full-Length PCSK9 Elicits a Higher Reduction in Plasma PCSK9 Than Similar Peptide-Based cVLP Vaccines Louise Goksøyr…Adam F. Sander. Vaccines 2023, 11(1), 2; IMMUN NANOPARTICLE-DERIVED VACCINES AGAINST POXVIRUSES, AND METHODS FOR MAKING AND USING THE SAME Patent WO/2024/049990 Publication Date 07.03.2024 Inventors MOSS, Bernard et al. IMMUN Rapid and Convenient Single-Chain Variable Fragment-Employed Electrochemical C-Reactive Protein Detection System Daimei Miura… Ryutaro Asano. Int. J. Mol. Sci. 2024, 25(5), 2859 Site-directed immobilization of enzymes on nanoparticles using self-assembly systems Haili Zhou…Fei Peng. Bioresour Technol 2024 Feb 28:397:130505. ENZ MagIC-Cryo-EM: Structural determination on magnetic beads for scarce macromolecules in heterogeneous samples Yasuhiro Arimura…Hironori Funabiki. bioRxiv 2024 Feb HIGH THROUGHPUT PEPTIDE IDENTIFICATION USING CONJUGATED BINDERS AND KINETIC ENCODING US patent 18466543 Publication Date 15.02.2024 Applicants Encodia, Inc. SEQUENCING Denaturing purifications demonstrate that PRC2 and other widely reported chromatin proteins do not appear to bind directly to RNA in vivo Jimmy K. Guo…Mitchell Guttman. Molecular Cell 21 February 2024 Mosaic sarbecovirus vaccination elicits cross-reactive responses in pre-immunized animals Alexander A. Cohen…Pamela J. Bjorkman. bioRxiv 2024 Feb IMMUN 975 LENGTH-ADAPTED CHIMERIC ANTIGEN RECEPTORS AND LENGTH-ADAPTED ACCESSORY RECEPTORS FOR IMPROVED CAR T CELL ACTIVATION Patent WO/2024/033642 Publication Date 15.02.2024 DUSHEK, Omer IMMUN Development and Immunological Evaluation of a Multiantigen Thermostable Nanovaccine Adjuvanted with T-Cell-Activating Scaffold for African Swine Fever Lidan Sun…Zeliang Chen. ACS Appl. Bio Mater. 2024 Feb IMMUN Irreversible light-activated SpyLigation mediates split-protein assembly in 4D Brizzia G. Munoz-Robles & Cole A. DeForest. Nature Protocols 2024 Jan MATERIALS OptoREACT: Optogenetic Receptor Activation on Nonengineered Human T Cells Anja Armbruster…Maximilian Hörner. ACS Synth. Biol. 2024 Feb IMMUN High-Level Secretory Production of Recombinant E2-Spy Antigen Protein via Combined Strategy in Pichia pastoris Bingkun Li…Ding Li. Fermentation 2024 Feb IMMUN Flow-Induced Microfluidic Assembly for Advanced Biocatalysis Materials Phillip Lemke…Christof M. Niemeyer. Advanced Functional Materials 24 January 2024 MATERIALS ENZ Nanoengineering Carboxysome Shells for Protein Cages with Programmable Cargo Targeting Tianpei Li…Lu-Ning Liu. ACS Nano 2024 Feb MATERIALS ENZ Active Template Synthesis of Protein [n]Catenanes Using Engineered Peptide–Peptide Ligation Tools Fan Zhang…Wen-Bin Zhang. CCS Chemistry Feb 2024 MATERIALS Development of plug-and-deliverable intracellular protein delivery platforms based on botulinum neurotoxin Seong Guk Park… Sebyung Kang. Int J Biol Macromol 2024 Jan 23;261(Pt 1):129622. ENZ Mechanically Tunable, Compostable, Healable and Scalable Engineered Living Materials Avinash Manjula-Basavanna…Anna Duraj-Thatte. bioRxiv 2024 Feb MATERIALS VIRAL PARTICLES RETARGETED TO TRANSFERRIN RECEPTOR 1 Patent WO/2024/026494 Publication Date 01.02.2024 REGENERON PHARMACEUTICALS, INC. [US]/[US] IMMUN De novo design of modular protein hydrogels with programmable intra- and extracellular viscoelasticity Rubul Mout…David Baker. PNAS 2024 Jan MATERIALS Exploration and Application of DNA-Binding Proteins to Make a Versatile DNA–Protein Covalent-Linking Patch (D-Pclip): The Case of a Biosensing Element Erika Komiya…Ryutaro Asano. J. Am. Chem. Soc. 2024 Jan Enhancing antibody responses by multivalent antigen display on thymus-independent DNA origami scaffolds Eike-Christian Wamhoff…Mark Bathe. Nature Communications volume 15, Article number: 795 (2024) MATERIALS IMMUN Spontaneous and site-specific immobilization of PNGase F via spy chemistry Liang Zhang…Song Wang. RSC Advances 2024 Jan ENZ Cryo-EM analysis on magnetic beads for scarce macromolecules in heterogeneous samples Yasuhiro Arimura…Hironori Funabiki. bioRxiv 2024 Jan MATERIALS Engineering multivalent Fc display for FcγR blockade Ekaterina Petrova…Ana I. Teixeira. bioRxiv 2024 Jan IMMUN Discovery and engineering of the antibody response against a prominent skin commensal Djenet Bousbaine…Michael A. Fischbach. bioRxiv 2024 Jan IMMUN Enhancing humoral and mucosal immune response of PED vaccine candidate by fusing S1 protein to nanoparticle multimerization Minghui Li…Gaiping Zhang. Veterinary Microbiology19 January 2024, 110003 IMMUN Photoactivatable Nanobody Conjugate Dimerizer Temporally Resolves Tiam1-Rac1 Signaling Axis Chengjian Zhou…Xi Chen. Advanced Science. 15 January 2024 OMPG VARIANTS Patent Application Number 18449904 Publication Date 11.01.2024 Applicants Roche Sequencing Solutions, Inc. SEQUENCING GENETICALLY ENGINEERED RECOMBINANT PROTEINS FOR FUNCTIONAL REGENERATIVE TISSUE SCAFFOLDS Patent Application Number 18365648 Publication Date 11.01.2024 Inventors Minkyu Kim et al. MATERIALS MOLECULAR PEPTIDE MUTANT Patent Application Number 18255108 Publication Date 04.01.2024 Applicants NANJING TECH UNIVERSITY Potent immune responses against thermostable Foot-and-Mouth disease virus VP1 nanovaccine adjuvanted with polymeric thermostable scaffold Yuanli Peng…Zeliang Chen. Vaccine 13 January 2024 IMMUN Affinity-assisted covalent self-assembly of PduQ-SpyTag and Nox-SpyCatcher to construct multi-enzyme complexes on the surface of magnetic microsphere modified with chelated Ni2+ Mianxing Luo…Guo Chen. International Journal of Biological Macromolecules 11 January 2024 ENZ 950 Light-based juxtacrine signaling between synthetic cells Hossein Moghimianavval…Allen P Liu. bioRxiv 2024 Jan Reengineering of an artificial protein cage for efficient packaging of active enzymes Yusuke Azuma…Jonathan G. Heddle. bioRxiv 2023 Dec ENZ Enhancing immune responses of ESC-based TAA cancer vaccines with a novel OMV delivery system Meiling Jin…Jian-Dong Huang. Journal of Nanobiotechnology volume 22, Article number: 15 (2024) IMMUN Hidden route of protein damage through confined oxygen gas Seoyoon Kim…Duyoung Min. bioRxiv 2024 Jan MATERIALS Preclinical Development of a Stabilized RH5 Virus-Like Particle Vaccine that Induces Improved Anti-Malarial Antibodies Lloyd D W King…Mark Howarth, Sumi Biswas, Simon J Draper. bioRxiv 2024 Jan IMMUN Cleavage-intermediate Lassa virus trimer elicits neutralizing responses, identifies neutralizing nanobodies, and reveals an apex-situated site-of-vulnerability Jason Gorman…Peter D. Kwong. Nature Communications volume 15, Article number: 285 (2024) IMMUN KARI NANOPARTICLE Patent WO/2024/006532 Publication Date 04.01.2024 OLMEDILLAS, Eduardo et al. Extraordinary Titer and Broad Anti-SARS-CoV-2 Neutralization Induced by Stabilized RBD Nanoparticles from Strain BA.5 Z. Wang…S. Wang. Vaccines 2024 IMMUN SpyDirect: A Novel Biofunctionalization Method for High Stability and Longevity of Electronic Biosensors Keying Guo…Sahika Inal. Advanced Science 31 December 2023 MATERIALS Display of PETase on the Cell Surface of Escherichia coli Using the Anchor Protein PgsA Takuma Yamashita…Hiroyasu Ogino. Applied Biochemistry and Biotechnology Jan 2024 ENZ Lipid nanodisc scaffold and size alter the structure of a pentameric ligand-gated ion channel Vikram Dalal…Wayland W. L. Cheng. Nature Communications volume 15, Article number: 25 (2024) Molecular Engineering of a Spider Silk and Mussel Foot Hybrid Protein Gives a Strong and Tough Biomimetic Adhesive Yin Yin…Markus B. Linder. Advanced Materials Interfaces 02 January 2024 MATERIALS METHODS FOR SPORE DISPLAY Patent WO/2023/245018 Publication Date 21.12.2023 Inventors NICKS, Trevor et al. MATERIALS IMMUN The SpyCatcher-SpyTag interaction mediates tunable anti-tumor cytotoxicity of NK cells. Guo C…Zhu W. Mol Immunol. 2023 Dec 20;165:11-18. IMMUN Ligand-Mediated Mechanical Enhancement in Protein Complexes at Nano- and Macro-Scale Samuel Kim…Minkyu Kim. ACS Appl. Mater. Interfaces 2023 MATERIALS De novo Semi-synthetic Platform for Monitoring Protein degradation in Live Cells Mahdi Hasan…Ashraf Brik. Chembiochem 2023 Nov 30:e202300731. Protein cage nanoparticle-based NK cell-engaging nanodrones (NKeNDs) effectively recruit NK cells to target tumor sites and suppress tumor growth Seong Guk Park…Sebyung Kang. Nanotoday 2024 Feb IMMUN Engineered immunogens to elicit antibodies against conserved coronavirus epitopes A. Brenda Kapingidza…Mihai L. Azoitei. Nature Communications volume 14, Article number: 7897 (2023) IMMUN METHODS AND COMPOSITIONS FOR DETERMINING THE ANTIGEN SPECIFICITY OF T CELLS Patent WO/2023/235761 Publication Date 07.12.2023 Inventors HACOHEN, Nir BAKALAR, Matthew IMMUN UNIVERSAL ADJUVANT FOR NASAL, ORAL, AND INTRAMUSCULAR DELIVERY OF VACCINES Patent WO/2023/230241 Publication Date 30.11.2023 DESIGN-ZYME LLC [US]/[US] IMMUN Encapsulation of AAV into protein vault nanoparticles as a novel solution to gene therapy's neutralizing antibody problem Logan Thrasher Collins…David Curiel. bioRxiv 2023 Nov IMMUN MATERIALS Accelerating the design of pili-enabled living materials using an integrative technological workflow Yuanyuan Huang…Chao Zhong. Nature Chemical Biology (2023) MATERIALS Enhancing anti-viral neutralization response to immunization with HIV-1 envelope glycoprotein immunogens Shamim Ahmed…Alon Herschhorn. npj Vaccines volume 8, Article number: 181 (2023) IMMUN Free Energy Landscape of Type III Fibronectin Domain with Identified Intermediate State and Hierarchical Symmetry Hao Sun…Hu Chen. Phys. Rev. Lett. 131, 218402 – Published 22 November 2023 MATERIALS Dimer stabilization by SpyTag/SpyCatcher coupling of the reductase domains of a chimeric P450 BM3 monooxygenase from Bacillus spp. improves its stability, activity and purification Arabella Essert, Kathrin Castiglione. ChemBioChem 2023 Nov ENZ 925 A candidate nanoparticle vaccine comprised of multiple epitopes of the African swine fever virus elicits a robust immune response Jinxing Song…Gaiping Zhang. Journal of Nanobiotechnology volume 21, Article number: 424 (2023) IMMUN VECTORS AND METHODS FOR IN VIVO ANTIBODY PRODUCTION Patent WO/2023/220603 Publication Date 16.11.2023 REGENERON PHARMACEUTICALS, INC. [US]/[US] IMMUN Study on the properties of a dual-system-based protein scaffold for orthogonal self-assembly Meng Zhang…Jun Zhao. International Journal of Biological Macromolecules16 November 2023, 127946 MATERIALS Development of a Tag/Catcher-mediated capsid virus-like particle vaccine presenting the conserved Neisseria gonorrhoeae SliC antigen that blocks human lysozyme Fabian G Martinez…Aleksandra E Sikora. Infect Immun 2023 Nov 2:e0024523. IMMUN Protein nanoscaffold enables programmable nanobody-luciferase immunoassembly for sensitive and simultaneous detection of aflatoxin B1 and ochratoxin A Shaowen Wu…Shijuan Yan. J Hazard Mater 2023 Oct 5:462:132701. ENZ A novel protein purification scheme based on salt inducible self-assembling peptides Guang Zeng…Zhanglin Lin. Microbial Cell Factories volume 22, Article number: 224 (2023) A BACTERIOPHAGE-BASED, NEEDLE AND ADJUVANT-FREE, MUCOSAL COVID-19 VACCINE Patent WO/2023/209556 Publication Date 02.11.2023 Inventors RAO, Venigalla B. et al. IMMUN VARIANTS OF THE GAIN DOMAIN OF ADHESION G PROTEIN-COUPLED RECEPTORS Patent WO/2023/213993 Publication Date 09.11.2023 Inventors MARFOGLIA, Matthieu et al. METHOD FOR BIOSYNTHESIS OF PROTEIN HETEROCATENANE Patent United States of America Application Number 17999377 Publication Date 02.11.2023 Inventors Wenbin ZHANG et al MATERIALS A modular approach to enhancing cell membrane-coated nanoparticle functionality using genetic engineering Nishta Krishnan…Liangfang Zhang. Nature Nanotechnology (2023) Oct IMMUN A nanoparticle vaccine displaying the ookinete PSOP25 antigen elicits transmission-blocking antibody response against Plasmodium berghei Guixiang Yao…Yaming Cao. Parasites & Vectors volume 16, Article number: 403 (2023) IMMUN Molecular imaging of bacterial outer membrane vesicles based on bacterial surface display Dávid Szöllősi…Krisztián Szigeti. Scientific Reports volume 13, Article number: 18752 (2023) CONJUGATE SYSTEMS AND METHODS OF USE THEREOF Patent Application Number 18301228 Publication Date 19.10.2023 Applicants David Curiel et al. IMMUN MODIFIED VIRAL PARTICLES FOR GENE THERAPY US patent Application Date 11.05.2023 Publication Date 26.10.2023 Inventors Paul Alexander HEPPENSTALL IMMUN LONG LIFETIME ALPHA-HEMOLYSIN NANOPORES US patent Application Number 18171969 Publication Date 19.10.2023 Applicants Roche Sequencing Solutions SEQUENCING Engineered Reversible Inhibition of SpyCatcher Reactivity Enables Rapid Generation of Bispecific Antibodies Christian Hentrich…Francisco Ylera. bioRxiv 2023 Oct IMMUN Mechanical disengagement of the cohesin ring Martina Richeldi…Maxim I. Molodtsov. Nature Structural & Molecular Biology (2023) Oct MATERIALS Thermostable T Cell Multiepitope Nanoparticle Antigens Inducing Potent Immune Responses against the Swine Fever Virus Lidan Sun…Zeliang Chen. ACS Infect. Dis. 2023, Oct IMMUN Toward Minimal Transcription–Translation Machinery Ting Wang and Yuan Lu. ACS Synth. Biol. 2023 Oct ENZ An Antibiotic Degrading Engineered Living Material Platform to Combat Environmental Antibiotic Resistance Gokce Ozkul…Ozgur Safak Seker. bioRxiv 2023 Oct MATERIALS HIGH THROUGHPUT PEPTIDE IDENTIFICATION USING CONJUGATED BINDERS AND KINETIC ENCODING Patent WO/2023/201365 Publication Date 19.10.2023 ENCODIA, INC. [US]/[US] SEQUENCING Engineering of an Optogenetic T Cell Receptor Compatible with Fluorescence-Based Readouts Vincent Idstein…Wolfgang W. Schamel. ACS Synth. Biol. 2023 Oct IMMUN Biological Applications of Synthetic Binders Isolated from a Conceptually New Adhiron Library Claudia D’Ercole…Ario de Marco. Biomolecules 2023, 13(10), 1533 Synthetic Par polarity induces cytoskeleton asymmetry in unpolarized mammalian cells JL Watson…E Derivery. Cell Volume 186, Issue 21, 12 October 2023, Pages 4710-4727.e35 Unveiling Mechanical Activation: GAIN Domain Unfolding and Dissociation in Adhesion GPCRs Chaoyu Fu…Jie Yan. Nano Lett. 2023 Oct MATERIALS 900 Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation Jiayu Liu…Jing Zhou. Nature Communications volume 14, Article number: 6457 (2023) MATERIALS Living Magnetotactic Microrobots Based on Bacteria with a Surface-Displayed CRISPR/Cas12a System for Penaeus Viruses Detection Haoxiang Chen…Lei Ren. ACS Appl. Mater. Interfaces 2023 Oct MATERIALS Accurate location of two conserved linear epitopes of PEDV utilizing monoclonal antibodies induced by S1 protein nanoparticles Minghui Li…Gaiping Zhang. Int J Biol Macromol 2023 Oct 5:127276. IMMUN MicroRNAs are necessary for the emergence of Purkinje cell identity Norjin Zolboot…Giordano Lippi. bioRxiv 2023 September BACTERIAL MICROCOMPARTMENT VIRUS-LIKE PARTICLES Patent Application Number 18033282 Publication Date 28.09.2023 Inventors Yong Quan Tan et al. GROWTH AND SITE-SPECIFIC ORGANIZATION OF MICRO-SCALE BIMOLECULAR DEVICES ON LIVING CELLS Patent Application Number 18003462 Publication Date 28.09.2023 Inventors Rebecca B. Schulman et al. Hypoallergenic chimeric virus-like particles for the development of shrimp tropomyosin allergen Pen m 1-specific blocking antibodies Supapich Chanasit…Alain Jacquet. Allergy 2023 September IMMUN Native Agarose Gels and Contact Blotting as Means to Optimize the Protocols for the Formation of Antigen–Ligand Complexes Claudia D’Ercole and Ario de Marco. Bioengineering 2023 September Two molecule force spectroscopy on ligand–receptor interactions Jiacheng Zuo…Hongbin Li. 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IMMUN SELF-ASSEMBLING CIRCULAR TANDEM REPEAT PROTEINS WITH INCREASED STABILITY US patent Application Number 17624208 Publication Date 29.12.2022 Inventors Barry L. Stoddard et al. MATERIALS Construction of cell factory through combinatorial metabolic engineering for efficient production of itaconic acid Jiao Feng…Kequan Chen. Microbial Cell Factories volume 21, 275 (2022) ENZ Construction of Bi-Enzyme Self-Assembly Clusters Based on SpyCatcher/SpyTag for the Efficient Biosynthesis of (R)-Ethyl 2- hydroxy-4-phenylbutyrate Jinmei Wang…Zhimin Ou. Biomolecules 2023, 13(1), 91 ENZ Engineering a minimal SilkCatcher/Tag pair compatible with SpyCatcher/Tag pair for the production of native-sized spider silk Ruxia Fan…Sesilja Aranko. bioRxiv Dec 2022 MATERIALS SpyDisplay: A Versatile Phage Display Selection System using SpyTag/SpyCatcher Technology Sarah-Jane Kellmann…Francisco Ylera. bioRxiv Dec 2022 IMMUN Normalizing the Immune Macroenvironment via Debulking Surgery to Strengthen Tumor Nanovaccine Efficacy and Eliminate Metastasis Nana Ma…Xiao Zhao. ACS Nano 2022 Dec IMMUN Spy&IAC enables specific capture of SpyTagged proteins for rapid assembly of plug-and-display nanoparticle vaccines Yilan Chen…Gaiping Zhang. International Journal of Biological Macromolecules 226, 31 January 2023, Pages 240-253 IMMUN Stapled NRPS enhances the production of valinomycin in Escherichia coli Shuhui Huang…Jian Li. Biotech and Bioeng. 12 December 2022 ENZ 725 Stimulation of the immune system by a tumor antigen bearing adenovirus-inspired VLP allows the control of melanoma growth S Besson…P Fender. Mol Therapy 2022 Dec IMMUN Improvement of Modular Protein Display Efficiency in SpyTag-Implemented Norovirus-like Particles Jirayu Boonyakida… Enoch Y. Park. Biomacromolecules December 7, 2022 IMMUN PROTEIN PARTICLES COMPRISING A DIPHTHERIA TOXIN CROSS REACTING MATERIAL (CRM) AMINO ACID SEQUENCE AND USES THEREOF Patent US No.20220378894 Publication Date 01.12.2022 Inventors Bernd REHM et al. IMMUN Construction and Functionalization of a Clathrin Assembly for a Targeted Protein Delivery Hong-Sik Kim…Hak-Sung Kim. Small 2022 Dec Plug-and-Display Photo-Switchable Systems on Plant Virus Nanoparticles Louisa Kauth…Christina Dickmeis. BioTech Nov 2022 MATERIALS Ultra-lightweight living structural material for enhanced stiffness and environmental sensing Heechul Park…Tim Lu. Materials Today Bio. 2022 Nov and bioRxiv MATERIALS VIRUSES WITH MODIFIED CAPSID PROTEINS Patent United States of America Application Number 17755447 Publication Date 24.11.2022 Applicants SpyBiotech Limited IMMUN RESURFACED DENGUE VIRUS AND ZIKI VIRUS GLYCOPROTEIN E DIII VARIANTS AND USES THEREOF Patent United States Number 17621513 Applicants Jonathan R. Lai et al. Nov 2022 IMMUN A modular and self-adjuvanted multivalent vaccine platform based on porcine circovirus virus-like nanoparticles Ze-Hui Liu…Fang He. Journal of Nanobiotechnology volume 20, Article number: 493 (2022) IMMUN DNA segment capture by Smc5/6 holo-complexes Michael Taschner…Stephan Gruber. bioRxiv 2022 Oct Lipid nanodisc scaffold and size alters the structure of a pentameric ligand-gated ion channel Vikram Dalal…Wayland W. L. Cheng. bioRxiv 2022 Nov The Ribosome Maturation Factor Rea1 utilizes nucleotide independent and ATP hydrolysis driven Linker remodelling for the removal of ribosome assembly factors Johan Busselez…Helgo Schmidt. bioRxiv 2022 Nov Fiber manipulation and post-assembly nanobody conjugation for adenoviral vector retargeting through SpyTag-SpyCatcher protein ligation Maryam Kadkhodazadeh…Arash Arashkia. Front Mol Biosci Nov 2022 IMMUN A multivalent antibody assembled from different building blocks using tag/catcher systems: A case study Christof Schindler…Thomas Langer. Protein Engineering, Design and Selection, 14 November 2022 MATERIALS Two-component vaccine consisting of virus-like particles displaying hepatitis C virus envelope protein 2 oligomers Jannick Prentoe…Adam F. Sander. npj Vaccines volume 7, Article number: 148 (2022) IMMUN Heavy-chain CDR3-engineered B cells facilitate in vivo evaluation of HIV-1 vaccine candidates Wenhui He…Michael Farzan. bioRxiv 2022 Nov IMMUN Orthogonal protein decoration of DNA nanostructures based on SpyCatcher-SpyTag interaction Sandra Kröll…Christof M. Niemeyer. Chem Comm 2022 Nov ENZ MATERIALS Adenovirus-Inspired Virus-like-Particles Displaying Melanoma Tumor Antigen Specifically Target Human DC Subsets and Trigger Antigen-Specific Immune Responses Solène Besson…Pascal Fender. Biomedicines 2022 Nov IMMUN Non-Neutralizing Epitopes Shade Neutralizing Epitopes against Omicron in a Multiple Epitope-Based Vaccine Hua-Rui Gong…Jian-Dong Huang. ACS Infect. Dis. 2022 Nov IMMUN ADENOVIRAL VECTORS AND VACCINES THEREOF Patent WO/2022/234276 Publication Date 10.11.2022 SPYBIOTECH LIMITED IMMUN Spatial organization of pathway enzymes via self-assembly to improve 2′-fucosyllactose biosynthesis in engineered Escherichia coli Roulin Chen…Wanmeng Mu. Biotech and Bioeng, 2022 Nov ENZ Antibody-mediated delivery of CRISPR-Cas9 ribonucleoproteins in human cells Stephanie Ubiparipovic…Romain Rouet. Protein Engineering, Design and Selection. Nov 2022 Directed assembly of genetically engineered eukaryotic cells into living functional materials via ultrahigh-affinity protein interactions Qikun Yi…Fei Sun. Science Advances, Nov 2022 MATERIALS PREFUSION-STABILIZED LASSA VIRUS GLYCOPROTEIN COMPLEX AND ITS USE Patent WO/2022/232648 Publication Date 03.11.2022 Inventors KWONG, Peter et al. IMMUN Improving the Synthesis Efficiency of Amino Acids Such as L-Lysine by Assembling Artificial Cellulosome Elements Dockerin Protein In Vivo Nan Li…Ruiming Wang. Fermentation 2022 Oct ENZ 700 Organizing Enzymes on Self-Assembled Protein Cages for Cascade Reactions Wei Kang…Chuang Xue. Ang Chem 2022 Oct ENZ Intranasal virus-particle mimicking vaccine enhances SARS-CoV-2 clearance in the Syrian hamster model Devanshi R Patel…Troy C Sutton. Vaccines 2024 and bioRxiv 2022 Oct IMMUN COMBINATION OF PEPTIDE LINKERS FOR PROTEIN COVALENT SELF-ASSEMBLY USING SPONTANEOUS ISOPEPTIDE BOND Patent WO/2022/222700 Publication Date 27.10.2022 ZHANG, Hui et al. Improving cross-protection against influenza virus in mice using a nanoparticle vaccine of mini-HA Hechao Zhu…Ping Qian. Vaccine Oct 2022 IMMUN Splicing factor BUD31 promotes ovarian cancer progression through sustaining the expression of anti- apoptotic BCL2L12 Zixiang Wang…Zhaojian Liu. Nature Communications 13, 6246 (2022) Plug-and-Display Photo-Switchable Systems on Plant Virus Nanoparticles Louisa Kauth…Christina Dickmeis. BioTech Oct 2022 MATERIALS The synergism of lytic polysaccharide monooxygenases with lichenase and their co-immobilization on silica nanospheres for green conversion of lichen biomass Lixi Cai…Guangya Zhang. Front. Nutr., 19 October 2022 ENZ NOVEL UNIVERSAL VACCINE PRESENTING SYSTEM Patent WO/2022/218997 Publication Date 20.10.2022 FENDER, Pascal et al. IMMUN Macromolecular assembly of bioluminescent protein nanoparticles for enhanced imaging Enya Li…Szu-Wen Wang. Materials Today, Oct 2022 IMAGING SpyStapler-mediated assembly of nanoparticle vaccines Songzi Kou…Fei Sun. Nano Research (2022) IMMUN Engineering synthetic microbial consortium for cadaverine biosynthesis from glycerol Simin Liu…Lei Zhang. Biotechnology Letters (2022) Oct ENZ Enhanced thermal stability of the β-galactosidase BgaB from Bacillus circulans by cyclization mediated via SpyTag/SpyCatcher interaction and its use in galacto-oligosaccharides synthesis Mingming Han… Yongqin Ni. Int Journal Biol Macromolecules Oct 2022 ENZ SpyPhage: A Cell-Free TXTL Platform for Rapid Engineering of Targeted Phage Therapies Sahan B. W. Liyanagedera…Vishwesh Kulkarni. ACS Syn Bio Oct 2022 MATERIALS Site-Specific and Tunable Co-immobilization of Proteins onto Magnetic Nanoparticles via Spy Chemistry Quanhui Ye…Na Wei. ACS Appl. Bio Mater. 2022 MATERIALS Heterologous Expression of Thermotolerant α-Glucosidase in Bacillus subtilis 168 and Improving Its Thermal Stability by Constructing Cyclized Proteins Zhi Wang…Zhiming Rao. Fermentation 2022, 8(10), 498 ENZ MULTIVALENT PROTEINS AND SCREENING METHODS WO/2022/200804 Publication Date 29.09.2022 LILIUMX Inventors SCHEU, Arne, KHAIRIL ANUAR, Irsyad, LIM, Sheryl Ying Ting MATERIALS Display of Streptococcus iniae α-Enolase on the Surface of Virus-Like Particles (VLPs) of Nervous Necrosis Virus (NNV) Using SpyTag/SpyCatcher Jeong In Yang & Ki Hong Kim. Marine Biotechnology (2022) IMMUN Cryo-EM reveals a mechanism of USP1 inhibition through a cryptic binding site Martin L. Rennie…Helen Walden. Science Advances 28 Sep 2022 A de novo matrix for macroscopic living materials from bacteria Sara Molinari…Caroline M. Ajo-Franklin. Nature Communications volume 13, Article number: 5544, 2022 Sept MATERIALS Mechanical Stabilization of a Bacterial Adhesion Complex Wenmao Huang…Jie Yan. J. Am. Chem. Soc. 2022, 144, 37, 16808–16818 Molecular engineering of a cryptic epitope in Spike RBD improves manufacturability and neutralizing breadth against SARS-CoV-2 variants Sergio A. Rodriguez-Aponte…J. Christopher Love. bioRxiv 2022 Sept IMMUN Directional immobilization of D-allulose 3-epimerase using SpyTag/SpyCatcher strategy as a robust biocatalyst for synthesizing D-allulose Xin Gao…Hui-Min Qin. Food Chem Sept 2022 ENZ Long-term retention of antigens in germinal centres is controlled by the spatial organisation of the follicular dendritic cell network Ana Martinez-Riano…Pavel Tolar, bioRxiv Sept 2022 IMMUN CD4-binding site immunogens elicit heterologous anti-HIV-1 neutralizing antibodies in transgenic and wildtype animals Harry B Gristick…Pamela J Bjorkman. bioRxiv Sept 2022 IMMUN Mechanical regulation of Titin N2B-us conformation and its binding to FHL2 Yuze Sun…Jie Yan. bioRxiv Sept 2022 MATERIALS 675 AN ARTIFICIAL PROTEIN-CAGE DECORATED WITH PARTICULAR MOLECULES ON THE EXTERIOR Patent WO/2022/182260 Publication Date 01.09.2022 HEDDLE, Jonathan et al. MATERIALS AN ARTIFICIAL PROTEIN-CAGE COMPRISING ENCAPSULATED THEREIN A GUEST CARGO Patent WO/2022/182262 Publication Date 01.09.2022 HEDDLE, Jonathan et al. MATERIALS ENDOTOXIN-FREE PRODUCTION OF RECOMBINANT SUBUNIT VACCINE COMPONENTS Patent WO/2022/175871 Publication Date 25.08.2022 INGENZA LTD [GB]/[GB] IMMUN Directing ricin-based immunotoxins with targeting affibodies and KDEL signal peptide to cancer cells effectively induces apoptosis and tumor suppression Seong Guk Park…Sebyung Kang. Journal of Nanobiotechnology volume 20, Article number: 387 (2022) Tailoring the Tag/Catcher System by Integrating Covalent Bonds and Noncovalent Interactions for Highly Efficient Protein Self-Assembly Yao Chen…Ling Jiang. Biomacromolecules 2022 MATERIALS ENZ Intracellular Assembly of Interacting Enzymes Yields Highly-Active Nanoparticles for Flow Biocatalysis Patrick Bitterwolf, Ahmed E. Zoheir, Julian Hertel, Sandra Kröll, Kersten S. Rabe, Christof M. Niemeyer. Chem Eur J Aug 2022 MATERIALS ENZ ADHESIVE PROTEIN Patent Application Number 17628132 Publication Date 18.08.2022 Katsutoshi Hori MATERIALS Single-step purification of functionalized protein nanostructures using multimodal chromatography Daniel L. Winter…Dominic J. Glover. J Bionanotechnology 2023 and bioRxiv MATERIALS Passive immunization with equine RBD-specific Fab protects K18-hACE2-mice against Alpha or Beta variants of SARS-CoV-2 Mariette Barbier…F. Heath Damron. Front Imm Aug 2022 IMMUN RBD-VLP Vaccines Adjuvanted with Alum or SWE Protect K18-hACE2 Mice against SARS-CoV-2 VOC Challenge Ting Y. Wong…F. Heath Damron. mSphere Aug 2022 IMMUN SPECIFIC SELECTION OF IMMUNE CELLS USING VERSATILE DISPLAY SCAFFOLDS US patent Application Number 17614711 Publication Date 04.08.2022 Scott Eugene LINDNER et al. IMMUN MODIFIED VIRAL PARTICLES AND USES THEREOF Patent Application Number 17612669 Publication Date 04.08.2022 Regeneron Pharmaceuticals, Inc. Valence-controlled protein conjugation on nanoparticles via re-arrangeable multivalent interactions of tandem repeat protein chains Hyeongjoo Choi, Yongwon Jung. Chem Sci Aug 2020 MATERIALS A "Plug-and-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-binding Domain Rang Feng…Hai-Bo Li. J Vis Exp 2022 Jul 25 IMMUN Protein Cages Engineered for Interaction-Driven Selective Encapsulation of Biomolecules Yeolin Lee…Yongwon Jung. ACS Appl. Mater. Interfaces Aug 2022 MATERIALS Interactions between mTORC2 core subunits Rictor and mSin1 dictate selective and context-dependent phosphorylation of substrate kinases SGK1 and Akt. Zanlin Yu…David Pearce. J Biol Chem Aug 2022 Bacterial biofilm functionalization through Bap amyloid engineering Leticia Matilla-Cuenca…Jaione Valle. npj Biofilms and Microbiomes 8:62 (2022) MATERIALS Convection Driven Ultrarapid Protein Detection via Nanobody-Functionalized Organic Electrochemical Transistors Anil Koklu…Sahika Inal. Advanced Materials July 2022 MATERIALS Development of a synthetic nanoparticle vaccine presenting the HIV-1 envelope glycoprotein Phindile Ximba…Edward Rybicki. Nanotechnology 26 July 2022 IMMUN Nanocarriers based on bacterial membrane materials for cancer vaccine delivery Xiao Zhao…Guangjun Nie. Nature Protocols (2022) July IMMUN Mechanical regulation of talin through binding and history-dependent unfolding Narayan Dahal…Ionel Popa. Science Advances 15 Jul 2022 MATERIALS TRAIL & EGFR affibody dual-display on a protein nanoparticle synergistically suppresses tumor growth Heejin Jun…Eunhee Kim. J Control Release 2022 Jul 6 MATERIALS Self-Assembling Protein Surfaces for In Situ Capture of Cell-Free-Synthesized Proteins Ella Lucille Thornton…Lynne Regan. Front. Bioeng. Biotechnol., 07 July 2022 MATERIALS Neutralizing monoclonal antibodies elicited by mosaic RBD nanoparticles bind conserved sarbecovirus epitopes Chengcheng Fan…Pamela J. Bjorkman. bioRxiv Jun 2022 IMMUN Steric accessibility of the N-terminus improves the titer and quality of recombinant proteins secreted from Komagataella phaffii Neil C. Dalvie…Christopher Love. bioRxiv Jun 2022 and Microb Cell Fact Sept 2022 IMMUN 650 Mosaic RBD nanoparticles protect against challenge by diverse sarbecoviruses in animal models Alexander A. Cohen…Pamela J. Bjorkman.. Science 5 Jul 2022 IMMUN Liquid–Liquid Phase Separation and Assembly of Silk-like Proteins is Dependent on the Polymer Length Laura Lemetti…A. Sesilja Aranko. Biomacromolecules 2022 Jul MATERIALS METALLOENZYMES FOR BIOMOLECULAR RECOGNITION OF N-TERMINAL MODIFIED PEPTIDES Patent WO/2022/147334 ENCODIA, INC. [US]/[US] ENZ SEQUENCING ANTIBODY-GUIDED PCSK9-MIMICKING IMMUNOGENS LACKING 9-RESIDUE SEQUENCE OVERLAP WITH HUMAN PROTEINS Patent WO/2022/147373 Publication Date 07.07.2022 KWONG, Peter D. et al. IMMUN Sequentially co-immobilized PET and MHET hydrolases via Spy chemistry in calcium phosphate nanocrystals present high-performance PET degradation Kun Chen…Yan Sun. Journal of Hazardous Materials. Jul 2022 MATERIALS SpySwitch enables pH- or heat-responsive capture and release for plug-and-display nanoassembly. Vester SK, Rahikainen R, Khairil Anuar INA, Hills RA, Tan TK, Howarth M. Nat Commun. 2022 Jun 28;13(1):3714. IMMUN A modular dCas9-based recruitment platform for combinatorial epigenome editing Tessa Swain…Ryan Lister. bioRxiv Jul 2022 and Nucleic Acids Research, gkad1108, 24 November 2023 NUCLEIC ACID BINDING PROTEIN FORMULATIONS AND USES THEREOF Patent WO/2022/140795 Publication Date 30.06.2022 SPOTLIGHT THERAPEUTICS [US]/[US] Multienzyme Assembly on Caveolar Membranes In Cellulo Qixin Wei…Jiang Xia. ACS Catal. 2022, 12, 8372–8379 ENZ NUCLEIC ACID VACCINES Patent WO/2022/129547 Publication Date 23.06.2022 UNIVERSITY OF COPENHAGEN [DK]/[DK] ADAPTVAC APS [DK]/[DK] IMMUN UNIVERSAL BACTERIOPHAGE T4 NANOPARTICLE PLATFORM TO DESIGN MULTIPLEX SARS-COV-2 VACCINE CANDIDATES BY CRISPR ENGINEERING Patent WO/2022/130196 Publication Date 23.06.2022 RAO, Venigalla B. ZHU, Jingen IMMUN Freeze-Drying of a Capsid Virus-like Particle-Based Platform Allows Stable Storage of Vaccines at Ambient Temperature Kara-Lee Aves…Adam F. Sander. Pharmaceutics June 2022 IMMUN Two-Dimensional Protein Nanoarray as a Carrier of Sensing Elements for Gold-Based Immunosensing Systems Jingya Tang…Dian-Bing Wang. Anal. Chem. June 21, 2022 MATERIALS Induction of Broadly Cross-Reactive Antibody Responses to SARS-CoV-2 Variants by S1 Nanoparticle Vaccines Cong Sun…Yin-Feng Kang. J Virol Jun 2022 IMMUN Peptide-Inspired One-Step Synthesis of Surface-Functionalized Fe3O4 Magnetic Nanoparticles for Oriented Enzyme Immobilization and Biocatalytic Applications Yanhong Zhou…Guangya Zhang. ACS Appl. Nano Mater. Jun 2022 ENZ DNA passes through cohesin’s hinge as well as its Smc3-kleisin interface James E Collier and Kim A Nasmyth. bioRxiv May 2022 and eLife Sept 2022 Increased Thermal Stability of the Carbonic Anhydrase Enzyme Complex for the Efficient Reduction of CO2 through Cyclization and Polymerization by Peptide Bonding Habin Sun…Jeong Eun Hyeon. Process Biochemistry, 6 June 2022 ENZ A Powassan virus domain III nanoparticle immunogen elicits neutralizing and protective antibodies in mice Ryan J. Malonis…Jonathan R. Lai. PLoS Path Jun 2022 IMMUN ENGINEERED PROBIOTIC COMPOSITIONS AND USES THEREOF Patent WO/2022/119926 Publication Date 09.06.2022 CUBILLOS, Andres Fernando, COLLINS, James J. Quaternary structure independent folding of voltage-gated ion channel pore domain subunits Cristina Arrigoni…Daniel L. Minor Jr. Nat Struct Mol Biol, Jun 2022 RECONSTITUTION OF A SPLIT-HALOTAG VIA ORTHOGONAL TAG-BINDING DOMAINS WO/2022/115696 Publication Date 02.06.2022 HUANG, Bo et al. MULTIPLEXED PROFILING OF RNA AND DNA MODIFICATIONS Patent WO/2022/115608 Publication Date 02.06.2022 ALIDA BIOSCIENCES, INC. [US]/[US] CRISPR-mediated protein-tagging signal amplification systems for efficient transcriptional activation and repression in Saccharomyces cerevisiae Haotian Zhai…Jin Hou. Nuc Acid Res May 2022 LIGHT-CONTROLLED VIRAL TRANSDUCTION Patent WO/2022/106690, Publication Date 27.05.2022 HÖRNER, Maximilian; WEBER, Wilfried Programmable protein topology via SpyCatcher-SpyTag chemistry in one-pot cell-free expression system Qingning Ye…Yuan Lu. Protein Science 21 May 2022 625 A loosened gating mechanism of RIG-I leads to autoimmune disorders Yixuan Lei…Jie Zheng. Nucleic Acid Res May 2022 IMMUN MULTIVALENT CARRIERS AND RELATED VACCINE COMPOSITIONS Patent WO/2022/103891 Publication Date 19.05.2022 COHEN, Alexander, A.; BJORKMAN, Pamela, J. IMMUN SCAFFOLDED ANTIGENS AND ENGINEERED SARS-COV-2 RECEPTOR-BINDING DOMAIN (RBD) POLYPEPTIDES WO/2022/104265 Publication Date 19.05.2022 THE SCRIPPS RESEARCH INSTITUTE [US]/[US] EMMUNE, INC. [US]/[US] IMMUN Non-Covalently Associated Streptavidin Multi-Arm Nanohubs Exhibit Mechanical and Thermal Stability in Protein-Network Materials David S Knoff…Minkyu Kim. Biomacromolecules Sept 2022 and bioRxiv May 2022 MATERIALS A Capsid Virus-Like Particle-Based SARS-CoV-2 Vaccine Induces High Levels of Antibodies and Protects Rhesus Macaques. Ariane Volkmann…Paul Chaplin. Front. Immunol. 05 April 2022 IMMUN SARS-COV-2 RBD CONJUGATED NANOPARTICLE VACCINE WO/2022/088953 Publication Date 05.05.2022 SUN YAT-SEN UNIVERSITY IMMUN A Magnetosome-Based Platform for Flow Biocatalysis Esther Mittmann…Dirk Schüler. ACS Appl. Mater. Interfaces May 2022 MATERIALS Free energy landscape of two-state protein Acylphosphatase with large contact order revealed by force- dependent folding and unfolding dynamics Xuening Ma…Hu Chen. Phys Rev, Jul 2022 and arXiv Mar 2022 MATERIALS A bacteriophage-based, highly efficacious, needle and adjuvant-free, mucosal COVID-19 vaccine Jingen Zhu…Venigalla B. Rao. mBio Jul 2022 and bioRxiv Apr 2022 IMMUN BACTERIAL MICROCOMPARTMENT VIRUS-LIKE PARTICLES Patent WO/2022/086450 Publication Date 28.04.2022 TAN, Yong Quan et al. METHODS AND COMPOSITIONS FOR RAPIDLY REPLACING CARDIAC MYOSIN BINDING PROTEIN-C IN SARCOMERES Patent Application Number 17559865 Publication Date 21.04.2022 Samantha P. Harris MATERIALS Evolutionary balance between foldability and functionality of a glucose transporter Hyun-Kyu Choi…Tae-Young Yoon. Nature Chemical Biology (Apr 2022) MATERIALS Estimation of microtubule-generated forces using a DNA origami nanospring Ali Nick Maleki…Vladimir A. Volkov. bioRxiv Apr 2022 MATERIALS BTB-BCL6 dimers as building blocks for reversible drug-induced protein oligomerization Lena Nitsch…MikołajSłabicki. Cell Reports Methods Apr 2022 An engineered live biotherapeutic for the prevention of antibiotic-induced dysbiosis Andrés Cubillos-Ruiz…James J. Collins. Nature Biomedical Engineering 2022 ENZ POLYPEPTIDE TERMINAL BINDERS AND USES THEREOF WO/2022/072560 Publication Date 07.04.2022 ENCODIA, INC. [US]/[US] SEQUENCING Engineering functional membrane-membrane interface by InterSpy Hossein Moghimianavval…Taekjip Ha. Small 26 May 2022 and bioRxiv 2022 Mar IMAGING Mosaic RBD nanoparticles protect against multiple sarbecovirus challenges in animal models Alexander A. Cohen…Pamela J. Bjorkman. bioRxiv April 2022 IMMUN B12-induced reassembly of split photoreceptor protein enables photoresponsive hydrogels with tunable mechanics Zhongguang Yang…Fei Sun. Science Advances Apr 2022 MATERIALS From 4-arm star proteins to diverse stimuli-responsive molecular networks enabled by orthogonal genetically encoded click chemistries Hong Kiu Francis Fok…Fei Sun. Polymer Chemistry Apr 2022 MATERIALS Effect of SpyTag/SpyCatcher cyclization on stability and refolding of green fluorescent protein Haiyan Song…Hui Luo. Biotechnology Letters Mar 2022 IMAGING POLYPEPTIDE TAGGED NUCLEOTIDES AND USE THEREOF IN NUCLEIC ACID SEQUENCING BY NANOPORE DETECTION Patent Application Number 17201834 Publication Date 24.03.2022 Roche Sequencing Solutions, Inc. SEQUENCING SpyChIP identifies cell type-specific transcription factor occupancy from complex tissues Siqian Feng, Richard S Mann. PNAS June 13, 2022 and bioRxiv Mar 2022 In vitro refolding of the structural protein VP1 of parvovirus B19 produces virus-like particles with functional VP1 unique region. Soto-Valerio, I.A….Bustos-Jaimes, I. Virology (2022) March IMMUN Comparison of Bacterial Expression Systems Based on Potato Virus Y-like Particles for Vaccine Generation by Anete Ogrina…Andris Zeltins. Vaccines 2022, 10(4), 485 IMMUN 301-600 publications here. 1-300 publications here.
It's the oldest question of all, George.  Who can spy on the spies?  ― John le Carré