SpyInfo
HOWARTH LAB
SOCIAL
ADDRESS
Department of Pharmacology,
University of Cambridge,
Tennis Court Road,
Cambridge
CB2 1PD
UK
CONTACT
e: mh2186@cam.ac.uk
t: 01223 334176
2023 Mark Howarth. All rights reserved.
Papers and Patents using Spy technology
Many of these publications don’t come up from a Pubmed search or citation search, so it should be useful
to bring them all together and hyperlinked (now >700 publications). Patents may be omitted if there is a journal
paper covering very similar work. Ones with particular relevance to
IMMUNOLOGY
,
MATERIALS
(polymers or
mechanobiology),
IMAGING
,
SEQUENCING
or
ENZYMES
are marked.
For an overview, please see our open-access review “
Power to the Protein
” from 2020.
SpyBank
is a database of >1,000 Tag/Catcher aa sequences to download (
Excel file
), from publications below.
SpyBank includes includes expression organism, Tag/Catcher location (N-terminal, internal, C-terminal) and
annotated linkers, as a reference for groups creating their own fusions. SpyBank itself is described in:
“Insider information on successful covalent
protein coupling with help from SpyBank.
Keeble AH, Howarth M. Meth Enz 2019
PDF
”.
(See note on codon usage at
Reagents
page).
301-600 below. Most recent papers
here
. Publications 1-300
here
.
600
Plug-and-Play Functionalization of Protein–Polymer Conjugates for Tunable Catalysis Enabled by Genetically Encoded “Click” Chemistry
Yushi Liu…Jian Li. ACS Catal. 2022, 12, 4165–4174 March 22, 2022
ENZ
Free energy landscape of two-state protein Acylphosphatase with large contact order revealed by force-dependent folding and unfolding dynamics
Xuening Ma…Hu Chen. arXiv Mar 2022
ENZ
COMPOSITIONS AND METHODS FOR SCREENING CELL INTERNALIZING AGENTS
WO/2022/056231 Publication Date 17.03.2022 SPOTLIGHT THERAPEUTICS
SYNTHETIC EXPRESSION SYSTEMS
Patent WO/2022/051696 Publication Date 10.03.2022 GINKGO BIOWORKS, INC. [US]/[US]
DE NOVO DESIGN OF PROTEIN SWITCHES FOR TUNABLE CONTROL OF PROTEIN DEGRADATION
Patent Application Number 17419413 Publication Date 10.03.2022 Inventors Robert LANGAN et al.
MATERIALS
SEQUENTIAL ENCODING METHODS AND RELATED KITS
Patent WO/2022/040098 Publication Date 24.02.2022 Applicants ENCODIA, INC.
SEQUENCING
TWO COMPONENT CO-ASSEMBLING TWO DIMENSIONAL PROTEIN STRUCTURES
Patent WO/2022/046626 Publication Date 03.03.2022 Applicants UNIVERSITY OF WASHINGTON
MATERIALS
Resurfaced ZIKV EDIII nanoparticle immunogens elicit neutralizing and protective responses in vivo
George I. Georgiev…Jonathan R. Lai. Cell Chemical Biology 28 February 2022
IMMUN
More efficient enzymatic cascade reactions by spatially confining enzymes via the SpyTag/SpyCatcher technology
Xuanru Zhong…Yonghua Wang. Molecular Catalysis Volume 521, March 2022, 112188
ENZ
Modular capsid decoration boosts adenovirus vaccine-induced humoral and cellular immunity against SARS-Co
V-2
Matthew D. J. Dicks…Mark Howarth, Sumi Biswas. Mol Ther Aug 2022 and bioRxiv Jan 2022
IMMUN
STReTCh: a strategy for facile detection of mechanical forces across proteins in cells
Brian L. Zhong, Vipul T. Vachharajani, Alexander R. Dunn. bioRxiv 2022
MATERIALS
Toward a glycyl radical enzyme containing synthetic bacterial microcompartment to produce pyruvate from formate and acetate
Henning Kirst…Cheryl A. Kerfeld. PNAS February 22, 2022 119 (8) e2116871119
ENZ
Protein-protein interactions enhance the thermal resilience of SpyRing-cyclized enzymes:
A molecular dynamic simulation study
Qi Gao, Dangling Ming. PLoS One February 17, 2022
ENZ
NUCLEOSIDE-5'-OLIGOPHOSPHATES TAGGED WITH POSITIVELY-CHARGED POLYMERS,
NANOPORES INCORPORATING NEGATIVE CHARGES, AND METHODS AND SYSTEMS
USING THE SAME
Patent WO/2022/033998 Publication Date 17.02.2022 HOFFMANN-LA ROCHE
SEQUENCING
EGFR Ligand Clustering on E2 Bionanoparticles for Targeted Delivery of
Chemotherapeutics to Breast Cancer Cells
Rachel M. Lieser…Wilfred Chen. Bioconjugate Chem. February 15, 2022
Cellular Synthesis of Protein Pretzelanes
Xilin Bai…Wen-Bin Zhang. Giant 2022 Feb
MATERIALS
HELICOBACTER PYLORI FERRITIN-BASED NOVEL CORONAVIRUS S PROTEIN MULTIMERIC
NANOVACCINE
Patent WO/2022/027702 Publication Date 10.02.2022 GUANGZHOU QIANYANG BIO-TECHNOLOGY PHARMACEUTICAL CO., LTD [CN]/[CN]
IMMUN
Programmable polymorphism of a virus-like particle
Artur P. Biela…Jonathan G. Heddle. Communications Materials 3, 7 (2022)
IMMUN
MATERIALS
Vaccine Candidate Double Mutant Variants of Enterotoxigenic Escherichia coli Heat-Stable Toxin
Ephrem Debebe Zegeye…Pål Puntervoll. Vaccines 2022, 10(2), 241
IMMUN
A Genetically Encoded Approach for Breaking Chromatin Symmetry
Bradley J. Lukasak…Tom W. Muir. ACS Cent. Sci. February 1, 2022
Cry3Aa*SpyCatcher Fusion Crystals Produced in Bacteria as Scaffolds for Multienzyme
Coimmobilization
Qian Sun…Michael K. Chan. Bioconjugate Chem. 2022
ENZ
Functional amyloid-chitin hybrid ink coupled with flexible fabrication approaches for diverse macro and micro-structures
Shicao Wei…Chao Zhong. Materials Today Bio Volume 13, January 2022, 100179
MATERIALS
Quaternary structure independent folding of voltage-gated ion channel pore domain
subunits
Cristina Arrigoni…Daniel L. Minor Jr. bioRxiv 2021
A de novo matrix for macroscopic living materials from bacteria
Sara Molinari…Caroline M. Ajo-Franklin. bioRxiv 2021
MATERIALS
Protease-controlled secretion and display of intercellular signals
Alexander E. Vlahos…Xiaojing J. Gao. bioRxiv 2021
ENZ
575
Mechano-Biochemical Regulation of the C. elegans HMP1–HMP2 protein
complex
Shimin Le…Jie Yan. bioRxiv 2021
MATERIALS
Enterotoxigenic Escherichia coli degrades the host MUC2 mucin barrier to facilitate
critical pathogen-enterocyte interactions in human small intestine
Alaullah Sheikh…James M. Fleckenstein. bioRxiv 2021
Gigavalent display of proteins on monodisperse polyacrylamide hydrogels as a
versatile modular platform for functional assays and protein engineering
Thomas Fryer…Florian Hollfelder. ACS Central Sci 2022 and bioRxiv 2021
MATERIALS
Molecular electronics sensors on a scalable semiconductor chip: A platform for
single-molecule measurement of binding kinetics and enzyme activity
Carl W. Fuller…Barry Merriman. PNAS Jan 2022
SEQUENCING
A cell-based phenotypic library selection and screening approach for the de novo
discovery of novel functional chimeric antigen receptors
Julie K. Fierle…Steven M. Dunn. Scientific Reports volume 12, Article number: 1136
(2022)
IMMUN
Incorporation of Endosomolytic Peptides with Varying Disruption Mechanisms into
EGFR-Targeted Protein Conjugates: The Effect on Intracellular Protein Delivery
and EGFR Specificity in Breast Cancer Cells
Rachel M. Lieser…Millicent O. Sullivan. Mol. Pharmaceutics January 18, 2022
Packaging of Diisopropyl Fluorophosphatase (DFPase) in Bacterial Outer Membrane
Vesicles Protects Its Activity at Extreme Temperature
Meghna Thakur…Scott A. Walper. ACS Biomater. Sci. Eng. 2022
ENZ
SpyTag/Catcher chemistry induces the formation of active inclusion bodies in E. coli
Wenge Dong…Hui Luo. International Journal of Biological Macromolecules 11
January 2022
MATERIALS
Molecular super-gluing: a straightforward tool for antibody labelling and its
application to mycotoxin biosensing
Fernando Pradanas-González…María C. Moreno-Bondi. Analytical and Bioanalytical Chemistry Jan 2022
IMMUN
NANOBODY FUNCTIONALIZED ELECTROCHEMICAL TRANSISTORS AND METHODS OF MAKING AND USING THEREOF
WO/2022/003651 Publication Date 06.01.2022 KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY [SA] Inv
entors INAL, Sahika et al.
MATERIALS
GROWTH AND SITE-SPECIFIC ORGANIZATION OF MICRO-SCALE BIMOLECULAR DEVICES ON LIVING CELLS
WO/2022/006055 Publication Date 06.01.2022 THE JOHNS HOPKINS UNIVERSITY [US] Inventors SCHULMAN, Rebecca B. et al.
MATERIALS
POLYPEPTIDE FRAGMENTS, IMMUNOGENIC COMPOSITION AGAINST SARS-CoV-2, AND IMPLEMENTATIONS THEREOF
WO/2022/003719 Publication Date 06.01.2022 Applicants INDIAN INSTITUTE OF SCIENCE [IN]/[IN], MYNVAX PRIVATE
LIMITED [IN]/[IN]
IMMUN
STABILIZED CORONAVIRUS SPIKE (S) PROTEIN IMMUNOGENS AND RELATED VACCINES
WO/2022/005503 Publication Date 06.01.2022 Applicants THE SCRIPPS RESEARCH INSTITUTE [US]/[US] Inventors HE,
Linling et al.
IMMUN
Personalized cancer vaccines from bacteria-derived outer membrane vesicles with antibody-mediated persistent
uptake by dendritic cells
Jie Liang…Guangjun Nie. Fundamental Research. Dec 2021
IMMUN
Adaptive Recombinant Nanoworms from Genetically Encodable Star Amphiphiles
Md Shahadat Hossain…Davoud Mozhdehi. Biomacromolecules December 23, 2021
MATERIALS
MODIFIED BACTERIAL MICROCOMPARTMENT SHELL PROTEIN
US patent Application Number 17458131 Dec 2021 Andrew R. Hagen et al.
MATERIALS
DNA-Encoded Multivalent Display of Chemically Modified Protein Tetramers on Phage: Synthesis and in
Vivo Applications
Guilherme M. Lima…Ratmir Derda. ACS Chem. Biol. 2021
MATERIALS
ENZ
Targeting Cross-Presentation as a Route to Improve the Efficiency of Peptide-Based Cancer Vaccines
Ben Wylie…Jason Waithman. Cancers 2021 Dec
IMMUN
CARRIER FOR IMMOBILIZING PROTEIN AND PREPARATION METHOD THEREFOR
Patent WO/2021/249433 Publication Date 16.12.2021 LIN, Zhanglin et al.
Rapid Production of Bispecific Antibodies from Off-the-Shelf IgGs with High Yield and Purity
Linghan Mei…Andrew Tsourkas. Bioconjugate Chem. Dec 2021
IMMUN
F-domain valency determines outcome of signaling through the angiopoietin pathway
Yan Ting Zhao…Hannele Ruohola-Baker. EMBO Reports (2021)22:e53471
MATERIALS
Antigenicity and immunogenicity of HA2 and M2e influenza virus antigens conjugated to norovirus-like, VP1 capsid-based particles
by the SpyTag/SpyCatcher technology
Suvi Heinimäki…Vesna Blazevic. Virology 566, January 2022, Pages 89-97
IMMUN
Amelioration of acute myocardial infarction injury through targeted ferritin nanocages loaded with an ALKBH5 inhibitor
Panke Cheng…Jianhong Tao. Acta Biomaterialia 2021
Engineering Bacillus subtilis for the formation of a durable living biocomposite material
Sun-Young Kang…Claudia Schmidt-Dannert. Nature Communications volume 12, Article number: 7133 (2021)
MATERIALS
Adding Dynamic Biomolecule Signaling to Hydrogel Systems via Tethered Photolabile Cell-Adhesive Proteins
Rachel Chapla…Jennifer L West. ACS Biomater Sci Eng 2021 Dec 6.
MATERIALS
Engineering Escherichia coli biofilm to increase contact surface for shikimate and L-malate production
Qiang Ding…Liming Liu. Bioresources and Bioprocessing volume 8, Article number: 118 (2021)
MATERIALS
550
Native llama Nanobody Library Panning Performed by Phage and Yeast Display Provides Binders Suitable for C-Reactive
Protein Detection
Sandra Oloketuyi…Ario de Marco. Biosensors 2021, 11, 496
IMMUN
BIOELECTRONIC DEVICES WITH PROGRAMMABLE ADAPTORS
Patent WO/2021/243113 Publication Date 02.12.2021 LINDSAY, Stuart et al.
SEQUENCING
Sequential immunization of macaques elicits heterologous neutralizing antibodies targeting the V3-glycan patch of HIV-1 Env
Amelia Escolano…Pamela J. Bjorkman. Science Translational Medicine • 24 Nov 2021 • Vol 13, Issue 621
IMMUN
High-resolution mapping of metal ions reveals principles of surface layer assembly in Caulobacter crescentus cells
Matthew Herdman…Tanmay A.M.Bharat. Structure Nov 2021
MATERIALS
A Modular System for the Rapid Comparison of Different Membrane Anchors for Surface Display on
Esch
erichia Coli
Sabrina Gallus…Kersten S Rabe. Chembiochem 2021 Nov 12.
ENZ
MODIFIED FILAMENTOUS FUNGI FOR PRODUCTION OF EXOGENOUS PROTEINS
Patent WO/2021/229483 Publication Date 18.11.2021 DYADIC INTERNATIONAL
IMMUN
PEPTIDE TAGS AND BINDING PARTNERS
Patent WO/2021/224451 Publication Date 11.11.2021 ADAPTVAC
IMMUN
Two energy barriers and a transient intermediate state determine the unfolding and folding dynamics
of cold shock protein
Haiyan Hong…Hu Chen. Communications Chemistry volume 4, Article number: 156 (2021)
MATERIALS
The specificity of the malarial VAR2CSA protein for chondroitin sulfate depends on 4-O-sulfation and
ligand accessibility
Charlotte B. Spliid…Thomas Mandel Clausen. J Biol Chem Nov 2021
IMMUN
IMAGING
RECOMBINANT HUMAN METAPNEUMOVIRUS F PROTEINS AND THEIR USE
US patent Publication Number WO/2021/222639 Publication Date 04.11.2021 KWONG, Peter et al.
IMMUN
Immunotargeting of Nanocrystals by SpyCatcher Conjugation of Engineered Antibodies
Cassio C. S. Pedroso, Bruce E. Cohen. ACS Nano October 25, 2021
IMAGING
Antibody elicited by HIV-1 immunogen vaccination in macaques displaces Env fusion peptide and destroys a neutralizing epitope
Morgan E. Abernathy…Pamela J. Bjorkman. npj Vaccines 6:126 (2021)
IMMUN
Protease-controlled secretion and display of intercellular signals
Alexander E. Vlahos…Xiaojing J. Gao. bioRxiv Oct 2021
CORONAVIRUS VACCINE
Patent WO/2021/210984 Publication Date 21.10.2021 HAAGMANS, Bartholomeus Leonardus et al.
IMMUN
Higher Order Protein Catenation Leads to an Artificial Antibody with Enhanced Affinity and In Vivo Stability
Wen-Hao Wu…Wen-Bin Zhang. J. Am. Chem. Soc. October 19, 2021
MATERIALS
Naive human B cells engage the receptor binding domain of SARS-CoV-2, variants of concern, and related
sarbecoviruses
Jared Feldman…Aaron G. Schmidt. Science Immunology • 14 Oct 2021
IMMUN
NAD(H)-PEG Swing Arms Improve Both the Activities and Stabilities of Modularly-Assembled Transhydrogenases Designed with Predictable Selectivities
Nadim Massad, Scott Banta. ChemBioChem 13 October 2021
ENZ
Two-Component Nanoparticle Vaccine Displaying Glycosylated Spike S1 Domain Induces Neutralizing Antibody Response against SARS-CoV-2 Variants
Linda van Oosten…Gorben P. Pijlman. mBio Oct 2021
IMMUN
MODIFIED DIPEPTIDE CLEAVASES, USES THEREOF AND RELATED KITS
Patent WO/2021/194908 Publication Date 30.09.2021 ENCODIA, INC.
SEQUENCI
NG
Programmable Polymorphism of a Virus-Like Particle
Artur Biela…Jonathan Heddle. Research Square 2021 Sept
Growth and site-specific organization of micron-scale biomolecular devices on living mammalian
cells
Sisi Jia…Rebecca Schulman. Nat Comm Sept 2021
MATERIALS
EXTRACELLULAR VESICLES FOR THERAPY
Patent WO/2021/189047 Publication Date 23.09.2021 CODIAK BIOSCIENCES, INC. [US]/[US]
Engineering Photoresponsive Ligand Tethers for Mechanical Regulation of Stem Cells
Junsheng Zhang…Yi Cao Advanced Materials 24 September 2021
Modeling-assisted Design of Thermostable Benzaldehyde Lyases from Rhodococcus erythropolis for Continuous Production of α-Hydroxy
Ketones
Martin Peng…Kersten S Rabe. ChemBioChem 2021 Sep 24.
ENZ
Elicitation of potent SARS-CoV-2 neutralizing antibody responses through immunization using a versatile adenovirus-inspired multimerization
platform
C Chevillard…P Fender. bioRxiv Sept 2021 and Mol Ther Feb 2022
IMMUN
525
An adjuvanted SARS-CoV-2 RBD nanoparticle elicits neutralizing antibodies and fully protective immunity in aged mice
Francesco Borriello…Ofer Levy. bioRxiv Sept 2021
IMMUN
One-step construction of circularized nanodiscs using SpyCatcher-SpyTag
Shanwen Zhang…Huan Bao. Nature Communications volume 12, Article number: 5451 (2021)
MATERIALS
Magnetic Nanoplatforms for Covalent Protein Immobilization Based on Spy Chemistry
Xiuyu Jin…Haifeng Gao. ACS Appl Mater Interfaces 2021 Sep 13.
MATERIALS
A Plant-Produced Virus-Like Particle Displaying Envelope Protein Domain III Elicits an Immune Response Against West Nile Virus in Mice
Jennifer Stander…Ann E. Meyers. Front. Plant Sci., 13 September 2021
IMMUN
Engineered SARS-CoV-2 receptor binding domain improves manufacturability in yeast and immunogenicity in mice
Neil C. Dalvie…J. Christopher Love. PNAS September 21, 2021 118 (38) e2106845118
IMMUN
Validation of an insertion-engineered isoprene synthase as a strategy to functionalize terpene synthases
C. Raul Gonzalez-Esquer…Cheryl A. Kerfeld. RSC Advances Sept 2021
ENZ
Oncofetal Chondroitin Sulfate Is a Highly Expressed Therapeutic Target in Non-Small Cell Lung Cancer
Htoo Zarni Oo…Mads Daugaard. Cancers 2021, 13, 4489
IMMUN
ALPHA-HEMOLYSIN VARIANTS AND USES THEREOF
US patent Application Number 17185021 Publication Date 02.09.2021 Roche Sequencing Solutions
SEQUENCING
Overproducing the BAM complex improves secretion of difficult-to-secrete recombinant autotransporter chimeras
Trang H. Phan…Peter van Ulsen. Microbial Cell Factories volume 20, Article number: 176 (2021)
IMMUN
Potent neutralization of SARS-CoV-2 including variants of concern by vaccines presenting the receptor-binding domain multivalently from
nanoscaffolds
Peter Halfmann…Ravi S. Kane. Bioengineering & Translational Medicine 27 August 2021
https://doi.org/10.1002/btm2.10253
IMMUN
Biofilm-Mediated Immobilization of a Multienzyme Complex for Accelerating Inositol Production
from Starch
Meixia Liu…Chun You. Bioconjugate Chem. September 1, 2021 https://doi.org/10.1021
ENZ
A Split-Cre system designed to detect simultaneous expression of two genes based on
SpyTag/SpyCatcher conjugation and Split-GFP dimerization
Xundong Wei…Jie Ma. J Biol Chem 2021 Aug
ENZ
SARS-COV-2 VACCINE
WO/2021/163365 Publication Date 19.08.2021 GRAHAM, Barney et al.
IMMUN
Orthogonal modular biosynthesis of nanoscale conjugate vaccines for vaccination against infection
Xin Li…Li Zhu. Nano Research Aug 2021
IMMUN
A Potential Method for One-step Purification and Direct Immobilization of Target Protein in Cell Lysate
with Magnetic Microbeads
Mianxing Luo…Hongwei Guo. Biochemical Engineering Journal Aug 2021
Structure of a Minimal α-Carboxysome-Derived Shell and Its Utility in Enzyme Stabilization
Yong Quan Tan…Wen Shan Yew. Biomacromolecules 2021 Aug
ENZ
Engineering Bacillus subtilis for the formation of a durable living biocomposite material
Sun-Young Kang…Claudia Schmidt-Dannert. bioRxiv Aug 2021
MATERIALS
COMPOSITIONS THAT REDUCE TEMPLATE THREADING INTO A NANOPORE
WO/2021/156370 Publication Date 12.08.2021 ROCHE
Gastrobodies are engineered antibody mimetics resilient to pepsin and hydrochloric acid
Niels Wicke, Mike R. Bedford & Mark Howarth. Comm Biol 4:960 (2021)
DE NOVO STABLE, MODULAR PD-1 BINDING PROTEINS AND OLIGOMERIC VARIANTS
Patent WO/2021/155132 Publication Date 05.08.2021 Applicants UWASHINGTON, AMGEN INC. [US]/[US] Inventors BRY
AN, Cassie et al.
IMMUN
A preparation strategy for protein-oriented immobilized silica magnetic beads with spy chemistry for ligand fishing
Yu Yi…GuoqingYing. Journal of Pharmaceutical Analysis Jul 2021
MATERIALS
DogCatcher allows loop-friendly protein-protein ligation
Anthony H.Keeble…MarkHowarth. Cell Chemical Biology July 2021
ENZ
IMAGING
THERMOSTABLE PETASE ENZYME
Patent WO/2021/145822 Publication Date 22.07.2021 GHADESSY, Farid John, SANA, Barindra
ENZ
Ultra-lightweight living structural material for enhanced stiffness and environmental sensing
Heechul Park…Timothy K. Lu. bioRxiv July 2021
MATERIALS
QUANTITATIVE CONTROL OF ACTIVITY OF ENGINEERED CELLS EXPRESSING UNIVERSAL IMMUNE RECEPTORS
Patent WO/2021/151038 Publication Date 29.07.2021 POWELL, Daniel J., Jr. et al.
IMMUN
500
ANTI 2019-NCOV VACCINE
Patent WO/2021/147025 Publication Date 29.07.2021 HUANG, Jian-Dong et al.
IMMUN
Protein-protein interactions enhance the thermal resilience of SpyRing enzymes: a
molecular dynamic simulation study
Qi Gao, Dengming Ming, arXiv July 2021
MODIFIED CLEAVASES, USES THEREOF AND RELATED KITS
US patent, Publication Date 15.07.2021 Encodia, Inc.
SEQUENCING
Improvement of a SARS-CoV-2 vaccine by enhancing the conjugation efficiency
of the immunogen to self-assembled nanoparticles
Xiantao Zhang…Hui Zhang. Cellular & Molecular Immunology (2021)
IMMUN
Computational design of a synthetic PD-1 agonist
Cassie M. Bryan…David Baker. PNAS July 2021
IMMUN
A high-specificity flap probe-based isothermal nucleic acid amplification method
based on recombinant FEN1-Bst DNA polymerase
Xin Ye…Jilie Kong. Biosensors and Bioelectronics 15 July 2021
ENZ
SARS-CoV-2 receptor binding domain displayed on HBsAg virus–like particles elicits protective immunity in macaques
Neil C. Dalvie…Dan H. Barouch. Science Advances Mar 2022 and bioRxiv
IMMUN
Naive human B cells engage the receptor binding domain of SARS-CoV-2, variants of concern,
and related sarbecoviruses
Jared Feldman…Aaron G. Schmidt. bioRxiv Jul 2021
IMMUN
METHODS FOR STABLE COMPLEX FORMATION AND RELATED KITS
Patent, Publication Date 08.07.2021 Encodia, Inc.
SEQUENCING
Cell-free Biosynthesis of Chlorogenic Acid Using a Mixture of Chassis Cell Extracts and
Purified Spy-Cyclized Enzymes
Fu-Xing Niu…Jian-Zhong Liu. J. Agric. Food Chem. 2021
ENZ
CARRIER MATRIX COMPRISING DODECIN PROTEIN
Patent WO/2021/136734 Publication Date 08.07.2021 GRININGER, Martin et al.
IMMUN
Self-assembling protein nanocages for modular enzyme assembly by orthogonal
bioconjugation
Emily A. Berckman…Wilfred Chen. Biotech Progress June 2021
ENZ
A bacterial extracellular vesicle-based intranasal vaccine against SARS-CoV-2 protects against
disease and elicits neutralizing antibodies to wild-type and Delta variants
Linglei Jiang…Kenneth W. Witwer. J Extracellular Vesicles14 March 2022 and bioRxiv
IMMUN
Rapid, convenient, and highly sensitive detection of human hemoglobin in serum using a high-affinity bivalent antibody–enzyme complex
Daimei Miura…Ryutaro Asano. Talanta June 2021
ENZ
Dendrimer-like supramolecular assembly of proteins with a tunable size and valency through stepwise iterative growth
Jin-Ho Bae…Hak-Sung Kim. Advanced Materials and bioRxiv June 2021
MATERIALS
Load and Display: Engineering Encapsulin as a Modular Nanoplatform for Protein-Cargo Encapsulation and
Protein-Ligand Decoration Using Split Intein and
SpyTag/SpyCatcher.
Hyukjun Choi…Sebyung Kang. Biomacromolecules, June 18, 2021
IMAGING
Green preparation of covalently co-immobilized multienzymes on silica nanoparticles for clean production
of reducing sugar from lignocellulosic biomass
Yuanqing Lin…Guangya Zhang. Journal of Cleaner Production 8 June 2021, 127994
ENZ
Spatiotemporally confined red light-controlled gene delivery at single-cell resolution using adeno-
associated viral vectors
Maximilian Hörner…Wilfried Weber. Science Advances June 2021
Development of a Modular Vaccine Platform for Multimeric Antigen Display Using an Orthobunyavirus
Model
by Andrea Aebischer…Martin Beer. Vaccines 2021, 9(6), 651
IMMUN
Facile tethering of stable and unstable proteins for optical tweezers experiments
Kevin Maciuba…Christian M. Kaiser. Biophys J 2021
MATERIALS
Talin mechanosensitivity is modulated by a direct interaction with cyclin-dependent kinase-1
Rosemarie E. Gough…Benjamin T. Goult. J Biol Chem Jun 2021
MATERIALS
Living fabrication of functional semi-interpenetrating polymeric materials
Zhuojun Dai…Lingchong You. Nature Communications volume 12, Article number: 3422 (2021)
MATERIALS
Sequential co-immobilization of multienzyme nanodevices based on SpyCatcher and SpyTag for robust biocatalysis
Fei Peng…Wen-Yong Lou. Mol Catalysis Jun 2021
ENZ
QUANTITATIVE ANALYSIS OF SINGLE-CELL PROTEINS BY IDENTIFICATION AND QUANTIFICATION SEPARATION (DUET)
Patent WO/2021/108459 Publication Date 03.06.2021 CAI, Long [US]/[US] et al.
BONDED NEUROTOXINS
Patent WO/2021/105664 Publication Date 03.06.2021 DAVLETOV, Bazbek LEESE, Charlotte Michelle
ENZ
475
Engineering a Blue Light Inducible SpyTag System (BLISS)
Emily J. Hartzell, Justin Terr, and Wilfred Chen. J. Am. Chem. Soc. 2021
IMAGING
Development of HER2-Targeting-Ligand-Modified Albumin Nanoparticles Based on the SpyTag/SpyCatcher System for
Photothermal Therapy
Changkyu Lee, Sebyung Kang. Biomacromolecules 2021 Jun 1.
MATERIALS
SpyTag/SpyCatcher Tether as a Fingerprint and Force Marker for Single-Molecule Force Spectroscopy Experiments
Zilong Guo…Hu Chen. Nanoscale 2021
MATERIALS
DETECTION OF OPTIMAL RECOMBINANTS USING FLUORESCENT PROTEIN FUSIONS
Patent WO/2021/100012 Publication Date 27.05.2021 INGENZA LTD
IMMUN
Immunogenicity and protective efficacy of a highly thermotolerant, trimeric SARS-CoV-2 receptor binding domain
derivative
Sameer Kumar Malladi…Raghavan Varadarajan. ACS Infect Disease 2021 and bioRxiv 2021
IMMUN
Development of a yeast cell surface display method using the SpyTag/SpyCatcher system
Kaho Kajiwara…Mitsuyoshi Ueda. Scientific Reports volume 11, Article number: 11059 (2021)
IMMUN
Target-switchable Gd(III)-DOTA/protein cage nanoparticle conjugates with multiple targeting affibody molecules as target selective T1 contrast agents for
high-field MRI
Hansol Kim…SebyungKang. Journal of Controlled Release, May 2021
IMAGING
Rapid single-molecule detection of COVID-19 and MERS antigens via nanobody-functionalized organic
electrochemical transistors
Keying Guo…Sahika Inal. Nature Biomedical Engineering (2021)
MATERIALS
IMMUN
Single-dose immunisation with a multimerised SARS-CoV-2 receptor binding domain (RBD) induces an enhanced
and protective response in mice
Ralf Salzer…Jan Löwe. bioRxiv May 2021
IMMUN
Head-to-Head Comparison of Modular Vaccines Developed Using Different Capsid Virus-Like Particle Backbones
and Antigen Conjugation Systems
Laurits Fredsgaard…Adam F. Sander. Vaccines 2021, 9(6), 539
IMMUN
Genetically Programmable Microbial Assembly
Mark T. Kozlowski…David A. Tirrell. ACS Synth. Biol. 2021
MATERIALS
Development of a VRC01-class germline targeting immunogen derived from anti-idiotypic antibodies
Emilie Seydoux…Andrew T. McGuire. Cell Reports 35 , 109084 May 2021
IMMUN
MyD88 oligomer size functions as a physical threshold to trigger IL1R Myddosome signalin
g
Rafael Deliz-Aguirre…Marcus J. Taylor. J Cell Biol (2021) 220 (7): e202012071.
IMAGING
Structures of a non-ribosomal peptide synthetase condensation domain suggest the basis of substrate selectivity
Thierry Izoré…Max J. Cryle. Nature Communications volume 12, Article number: 2511 (2021)
ENZ
Refocusing the Immune Response to Selected Epitopes on a Zika Virus Protein Antigen by Nanopatterning
Ana Castro…Ravi S. Kane. Advanced Healthcare Materials April 2021
IMMUN
CaMKII activation persistently segregates postsynaptic proteins via liquid phase separation
Tomohisa Hosokawa…Mingjie Zhang & Yasunori Hayashi. Nature Neuroscience April 2021
IMAGING
Activation of von Willebrand factor via mechanical unfolding of its discontinuous autoinhibitory module
Nicholas A. Arce…Renhao Li. Nature Communications 12: 2360 (2021)
MATERIALS
Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor binding domain in engineered Komagataella phaffii
Neil C. Dalvie…J. Christopher Love. bioRxiv Apr 2021
IMMUN
Chemogenetic Evolution of a Peroxidase-like Artificial Metalloenzyme
Ulrich Markel…Ulrich Schwaneberg. ACS Catal. Apr 2021, 11, 5079–5087
ENZ
Fusion peptide priming reduces immune responses to HIV-1 envelope trimer base
Angela R.Corrigan…John R.Mascola. Cell Reports Apr 2021
IMMUN
Bioengineered bacteria-derived outer membrane vesicles as a versatile antigen display platform for tumor vaccination via Plug-
and-Display technology
Keman Cheng…Guangjun Nie. Nature Communications 12: 2041 (Apr2021)
IMMUN
Development of a bispecific immune engager using a recombinant malaria protein
Mie A. Nordmaj…Ali Salanti. Cell Death & Disease volume 12, Article number: 353 (2021)
IMMUN
Ordered Coimmobilization of Multimeric Enzyme Arrays with Enhanced Biocatalytic Cascade Performance
Mohamed Yassin Ali…Yan Feng. ACS Appl. Bio Mater. 2021
ENZ
Structural basis of FANCD2 deubiquitination by USP1−UAF1
Martin L. Rennie…Helen Walden. Nature Structural & Molecular Biology (2021)
ENZ
Structural and functional analysis of LIM domain-dependent recruitment of paxillin to αvβ3 integrin-positive focal
adhesions
Marta Ripamonti…Bernhard Wehrle-Haller. Communications Biology Mar 2021
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Encapsulin Nanocontainers as Versatile Scaffolds for the Development of Artificial Metabolons
Matthew C. Jenkins and Stefan Lutz. ACS Synth. Biol. 2021 Mar
ENZ
Vaccine Efficacy of Self-Assembled Multimeric Protein Scaffold Particles Displaying the Glycoprotein Gn Head Domain
of Rift Valley Fever Virus
Paul J. Wichgers Schreur…Jeroen Kortekaas. Vaccines 2021, 9(3), 301
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Programming Integrative Multienzyme Systems and Ionic Strength For Recyclable Synthesis of Glutathione
Xiangwei Cui…Zhimin Li. J. Agric. Food Chem. Mar 2021
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Engineering High-Yield Biopolymer Secretion Creates an Extracellular Protein Matrix for Living Materials
Maria Teresa Orozco-Hidalgo…Caroline M. Ajo-Franklin. mSystems Mar 2021
MATERIALS
Exploration of a Sequential Gp140-Gp145 Immunization Regimen with Heterologous Envs to Induce a Protective
Cross-Reactive HIV Neutralizing Antibody Response In Non-human Primates
Xiangqing Ding…Jianqing Xu. Virologica Sinica. Mar 2021
IMMUN
Controlled and Stable Patterning of Diverse Inorganic Nanocrystals on Crystalline Two-Dimensional Protein Arrays
Victor R. Mann…Caroline M. Ajo-Franklin, Bruce E. Cohen. Biochemistry March 10, 2021
MATERIALS
Engineered SARS-CoV-2 receptor binding domain improves immunogenicity in mice and elicits protective immunity in
hamsters
Neil C. Dalvie…Darrell J. Irvine, J. Christopher Love. bioRxiv Mar 2021
IMMUN
ADENO-ASSOCIATED VIRUSES AND METHODS AND MATERIALS FOR MAKING AND USING ADENO-ASSOCIATED VIRUSES
Patent WO/2021/041951 Publication Date 04.03.2021 BYRNE, Leah et al.
Efficient biosynthesis of (2S, 3R)-4-methylsulfonylphenylserine by artificial self-assembly of enzyme complex combined
with an intensified acetaldehyde elimination system
Lian Xu…Juan Lin. Bioorganic Chemistry 24 February 2021
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DNA-Encoded Multivalent Display of Protein Tetramers on Phage: Synthesis and In Vivo Aplications
Guilherme M Lima…Ratmir Derda. bioRxiv Feb 2021
Improving the thermostability of trehalose synthase from Thermomonospora curvata by covalent cyclization using
peptide tags and investigation of the underlying molecular mechanism
Yao Chen…Ling Jiang. International Journal of Biological Macromolecules 168, 31 January 2021, 13-21
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One-step purification and immobilization of recombinant proteins using SpyTag/SpyCatcher chemistry
Junwei Tian…Hui Luo. Biotechnology Letters (2021)
Accessibility-dependent topology studies of membrane proteins using a SpyTag/SpyCatcher protein-ligation system
Yoonji Bae…Sebyung Kang. Int J Biol Macromol 2021 Feb 4;S0141-8130(21)00285-3.
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A novel all-in-one strategy for purification and immobilization of β-1,3-xylanase directly from cell lysate as active and recyclable
nanobiocatalyst
Lixi Cai…Guangya Zhang . Microbial Cell Factories volume 20, Article number: 37 (2021)
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The Immunogenicity of Capsid-Like Particle Vaccines in Combination with Different Adjuvants Using Different Routes of
Administration
Christoph M. Janitzek…Adam F. Sander. Vaccines 2021, 9(2), 131
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‘NAD-display’: Ultrahigh-Throughput in Vitro Screening of NAD(H) Dehydrogenases Using Bead Display & Flow Cytometry
Laurens Lindenburg and Florian Hollfelder. Ang Chemie Jan 2021
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SpyCatcher-SpyTagged ApxIA Toxoid and the Immune-Modulating Yeast Ghost Shells.
Seonhyung Lee; Beom-Ku Han; Yang-Hoon Kim; Ji-Young Ahn. J Biomedical Nanotechnology 2021-1-20
IMMUN
A Universal Bacteriophage T4 Nanoparticle Platform to Design Multiplex SARS-CoV-2 Vaccine Candidates by CRISPR Engineering
Jingen Zhu…Venigalla B. Rao. bioRxiv Jan 2021
IMMUN
Modular vaccine platform based on the norovirus-like particle
Vili Lampinen…Vesa P. Hytönen. Journal of Nanobiotechnology 19, 25 (2021)
IMMUN
Rapid Development of SARS-CoV-2 Spike Protein Receptor-Binding Domain Self-Assembled Nanoparticle Vaccine Candidates
Yin-Feng Kang…Mu-Sheng Zeng. ACS Nano January 19, 2021
IMMUN
Evidence for S2 flexibility by direct visualization of quantum dot–labeled myosin heads and rods within smoot
h muscle myosin filaments moving on actin in
vitro
Richard K. Brizendine…Christine R. Cremo. J Gen Physiol (2021) 153 (3): e202012751
IMAGING
Mosaic nanoparticles elicit cross-reactive immune responses to zoonotic coronaviruses in mice
Alexander A. Cohen…Pamela J. Bjorkman. Science 12 Jan 2021
IMMUN
Construction of a Self-Purification and Self-Assembly Coenzyme Regeneration System for the Synthesis of
Chiral Drug Intermediates
Wei Jiang and Wanru Zeng. ACS Omega January 12, 2021
ENZ
High-efficacy subcellular micropatterning of proteins using fibrinogen anchors
Joseph L. Watson…Emmanuel Derivery. J Cell Biol (2021) 220 (2): e202009063.
MATERIALS
Understanding condensation domain selectivity in non-ribosomal peptide biosynthesis: structural
characterization of the acceptor bound state
Max Cryle…Colin Jackson. Research Square Jan 2021
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Multiplexed Affinity Characterization of Protein Binders Directly from a Crude Cell Lysate by Covalent
Capture on Suspension Bead Arrays
Tuomas Huovinen, Laurens Lindenburg, Ralph Minter, and Florian Hollfelder. Analytical Chem Jan 2021
IMMUN
Ferritin nanoparticle based SARS-CoV-2 RBD vaccine induces persistent antibody response and long-term memory in mice
Wenjun Wang, Baoying Huang, Yanping Zhu, Wenjie Tan, Mingzhao Zhu. bioRxiv Dec 2020, Cell Mol Immunol 2021
IMMUN
Multiplex Single-Molecule Kinetics of Nanopore-Coupled Polymerases
Mirkó Palla…George M. Church. ACS Nano December 28, 2020
SEQUENCING
NANOPORE ASSEMBLIES AND USES THEREOF
US patent Application Number 16969518 Publication Date 24.12.2020 Oxford Nanopore
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MODIFIED VIRAL PARTICLES AND USES THEREOF
Patent WO/2020/242984 Publication Date 03.12.2020 REGENERON PHARMACEUTICALS, INC. [US]/[US]
Identifying Cleaved and Noncleaved Targets of Small Interfering RNAs and MicroRNAs in Mammalian Cells by SpyCLIP
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METHODS AND COMPOSITIONS FOR DELIVERY OF MOLECULE AND COMPLEXES TO REACTION SITES
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DISPLAY OF HETEROLOGOUS MOLECULES ON BACTERIAL CELLS AND MEMBRANE VESICLES
US patent Publication Date 03.12.2020 Applicants ABERA BIOSCIENCE AB
IMMUN
ANTI-TEM1 ANTIBODIES AND ANTIGEN-BINDING PORTIONS THEREOF
Patent WO/2020/243455 Publication Date 03.12.2020 DUNN, Steven, M. et al.
Molecular origin of the extreme mechanical strength of an ester bond containing pathogen surface protein
Hai Lei…Yi Cao. Nature Communications 12, 5082 (2021) and ResearchSquare Dec 2020
MATERIALS
Versatile labeling and detection of endogenous proteins using tag-assisted split enzyme complementation
Suraj Makhija…Bo Huang. bioRxiv Dec 2020 and ACS Chem Biol Mar 2021
IMAGING
Assembly principles and stoichiometry of a complete human kinetochore module
Kai Walstein…Andrea Musacchio. Science Advances Jun 2021, bioRxiv Dec 2020
Protein folding modulates the chemical reactivity of a Gram-positive adhesin
Alvaro Alonso-Caballero…Julio M. Fernandez. Nature Chemistry (Nov 2020)
MATERIALS
Nanoparticle Vaccines Based on the Receptor Binding Domain (RBD) and Heptad Repeat (HR) of SARS-CoV-2 Elicit
Robust Protective Immune Responses
Xiancai Ma…Hui Zhang. Immunity November 25, 2020
IMMUN
Catalytically Active Bioseparation Resin Utilizing a Covalent Intermediate for Tagless Protein Purification
Ben Evert, Ben Vezina, and Bernd H. A. Rehm. ACS Appl. Bio Mater. Nov 2020
MATERIALS
An engineered receptor-binding domain improves the immunogenicity of multivalent SARS-CoV-2 vaccines
Brian D. Quinlan…Michael Farzan. mBio May 2021 and bioRxiv Nov 2020
IMMUN
Mosaic RBD nanoparticles elicit neutralizing antibodies against SARS-CoV-2 and zoonotic coronaviruses
Alexander A. Cohen…Pamela J. Bjorkman. bioRxiv Nov 2020
IMMUN
Nimotuzumab Site-Specifically Labeled with 89Zr and 225Ac Using SpyTag/SpyCatcher for PET Imaging
and Alpha Particle Radioimmunotherapy of Epidermal Growth Factor Receptor Positive Cancers
by Viswas Raja Solomon…Humphrey Fonge. Cancers 2020, 12(11), 3449
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A nanobody-functionalized organic electrochemical transistor for the rapid detection of SARS-CoV-2
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nd MERS antigens at the physical limit
Keying Guo…Sahika Inal. medRxiv Nov 2020
IMMUN
Cryo-EM analysis of human mitochondrial Hsp90 in multiple tetrameric states
Yanxin Liu…David A. Agard. bioRxiv Nov 2020
IMAGING
Behavior control of membrane-less protein liquid condensates with metal ion-induced phase
separation
Kibeom Hong, Daesun Song & Yongwon Jung. Nature Communications 11: 5554 (2020)
MATERIALS
Ni-modified magnetic nanoparticles for affinity purification of His-tagged proteins from the complex
matrix of the silkworm fat body
Robert Minkner…Enoch Y. Park. Journal of Nanobiotechnology volume 18, Article number: 159 (2020)
Mycobacterial surface-associated ESX-1 virulence factors play a role in mycobacterial adherence and
invasion into
lung epithelial cells
Yanqing Bao…Jianjun Sun. bioRxiv Oct 2020
On demand MyD88 oligomerization is controlled by IRAK4 during Myddosome signaling
Rafael Deliz-Aguirre…Marcus J. Taylor. bioRxiv 2020
IMAGING
F-domain valency determines outcome of signaling through the angiopoietin pathway
Yan Ting Zhao…Hannele Ruohola-Baker. bioRxiv 2020
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Rapid development of SARS-CoV-2 receptor binding domain-conjugated nanoparticle vaccine candidate
Yin-Feng Kang…Mu-Sheng Zeng. bioRxiv Nov 2020
IMMUN
A DNA-nanoassembly-based approach to map membrane protein nanoenvironments
Elena Ambrosetti…Ana I. Teixeira. Nature Nanotechnology (2020)
MATERIALS
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Dual-Functionalizable Streptavidin–SpyCatcher-Fused Protein–Polymer Hydrogels as Scaffolds for Cell Culture
Kosuke Minamihata…Noriho Kamiya. ACS Appl. Bio Mater. 2020
MATERIALS
IN VIVO IMMOBILIZATION OF PROTEINS
WO/2020/216322 Publication Date 29.10.2020 Inventors CHAN, Michael et al.
MATERIALS
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AAV VECTORS ENCODING MINI-PCDH15 AND USES THEREOF
WO/2020/219990 Publication Date 29.10.202 Inventors COREY, David, P. et al.
Directed co-evolution of interacting protein–peptide pairs by compartmentalized two-hybrid replication (C2HR)
Jia Wei Siau…Farid J Ghadessy. Nucleic Acids Research, 26 October 2020
A platform incorporating trimeric antigens into self-assembling nanoparticles reveals SARS-CoV-2-spike nanoparticles to elicit
substantially higher neutralizing responses than spike alone
. Baoshan Zhang…Peter D. Kwong. Scientific Reports 10,
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IMMUN
Chemically Orthogonal Protein Ligation Domains for Independent Control of Hydrogel Modification with Adhesive Ligands
and Growth Factors
Joshua A. Hammer and Jennifer L. West. Bioconjugate Chem. Oct 2020
MATERIALS
The Spy that links: Creation of nonlinear protein architectures and materials using SpyTag/SpyCatcher chemistry
Ri Wang, Fei Sun. Methods in Enzymology Oct 2020
MATERIALS
Structural stability of SARS-CoV-2 degrades with temperature
A. Sharma…M.D.Vershinin. bioRxiv Oct 2020
IMMUN
Covalent protein display on Hepatitis B core-like particles in plants through the in vivo use of the SpyTag/SpyCatcher system
Hadrien Peyret…George P. Lomonossoff. Scientific Reports volume 10, Article number: 17095 (2020)
IMMUN
Exploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigens
Lucille F. van Beek …Marien I. de Jonge. Virulence 2020
IMMUN
Injectable, photoresponsive hydrogels for delivering neuroprotective proteins enabled by metal-directed protein assembly
Bojing Jiang…Fei Sun. Sci Adv Oct 2020
MATERIALS
MULTIVALENT MALARIA TRANSMISSION-BLOCKING VACCINES
WO/2020/201191 Publication Date 08.10.2020 STATENS SERUM INSTITUT [DK]/[DK] KØBENHAVNS UNIVERSITET [DK]/[DK]
IMMUN
Conditional Protein Rescue by Binding-Induced Protective Shielding
Andrew S. Gaynor and Wilfred Chen. ACS Synth. Biol. October 7, 2020
Exploring metal availability in the natural niche of Streptococcus pneumoniae to discover potential vaccine antigens
Lucille F. van Beek…Marien I. de Jonge. Virulence Oct 2020
IMMUN
TARGETED ACTIVE GENE EDITING AGENT AND METHODS OF USE
WO/2020/198151 Publication Date 01.10.2020 SPOTLIGHT THERAPEUTICS [US]/[US]
ENZ
Synthetic Multienzyme Complexes Assembled on Virus-like Particles for Cascade Biosynthesis In Cellulo
Qixin Wei, Sicong He, Jianan Qu, and Jiang Xia. Bioconjugate Chem. Oct 2020
ENZ
MATERIALS
ANTIGEN BINDING FRAGMENTS CONJUGATED TO A PLURALITY OF FC ISOTYPES AND SUBCLASSES
Patent WO/2020/188356 24.09.2020 BIO-RAD ABD SEROTEC GMBH [DE]/[DE]
IMMUN
PROTECTION OF SPYTAG-CONTAINING PERIPLASMIC FUSION PROTEINS FROM PROTEASE TSP AND OMPT
DEGRADATION
Patent WO/2020/188350 24.09.2020 BIO-RAD ABD SEROTEC GMBH [DE]/[DE]
IMMUN
ANTIGEN BINDING PROTEINS
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IMMUN
Design of Biologically Active Binary Protein 2D Materials
Ariel J Ben-Sasson…David Baker. bioRxiv Sept 2020, Nature Jan 2021
MATERIALS
Periplasmic Expression of SpyTagged Antibody Fragments Enables Rapid Modular Antibody Assembly
Christian Hentrich…Francisco Ylera. bioRxiv Sept 2020
IMMUN
IMAGING
Site-Specific Linking of an Oligonucleotide to Mono- and Bivalent Recombinant Antibodies with SpyCatcher-SpyTag System for Immuno-PCR
Anastasiia Kushnarova-Vakal*, Antti Äärelä, Tuomas Huovinen, Pasi Virta, and Urpo Lamminmäki. ACS Omega 2020, September 19, 2020
IMMUN
Self-assembling nanoparticles presenting receptor binding domain and stabilized spike as next-gene
ration COVID-19 vaccines
Linling He…Ian A. Wilson, Jiang Zhu. bioRxiv Sept 2020 and Sci Adv Mar 2021
IMMUN
375
Modular Hepatitis B Virus-Like Particle Platform for Biosensing and Drug Delivery
Emily J Hartzell, Rachel M Lieser, Millicent O. Sullivan, and Wilfred Chen. ACS Nano Sept 2020
Modulating mechanical stability of heterodimerization between engineered orthogonal helical
domains
Miao Yu… Jie Yan. Nature Communications volume 11, Article number: 4476 (2020)
MATERIALS
General and robust covalently linked graphene oxide affinity grids for high-resolution cryo-EM
Feng Wang…David A. Agard. PNAS September 10, 2020
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IMAGING
Multivalent Interaction-Driven Assembly of Discrete, Flexible, and Asymmetric Supramolecular
Protein Nano-Prisms
Suyeong Han…Yongwon Jung. Ang. Chemie Aug 2020
MATERIALS
Overcoming Symmetry Mismatch in Vaccine Nanoassembly via Spontaneous Amidation
Rolle Rahikainen, Pramila Rijal, Tiong Kit Tan, Hung-Jen Wu , Anne-Marie C. Andersson, Jordan R.
Barrett, Thomas A. Bowden, Simon J. Draper, Alain R. Townsend, Mark Howarth. Angewandte
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IMMUN
Serological screening of influenza A virus antibodies in cats and dogs indicates frequent infection with different subtypes
Shan Zhao…Herman Egberink. J Clin Microbiol Sept 2020
IMMUN
Engineering high-yield biopolymer secretion creates an extracellular protein matrix for living materials
Marimikel Charrier…Caroline M. Ajo-Franklin. bioRxiv Sept 2020
MATERIALS
Tagging and catching: rapid isolation and efficient labeling of organelles using the covalent Spy-System in planta
Martina Lang, Marlene Pröschel, Nico Brüggen & Uwe Sonnewald. Plant Methods volume 16, Article number: 122 (2020)
A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain
induces potent neutralising antibody responses
Tiong Kit Tan, Pramila Rijal…Mark Howarth, Alain Townsend. bioRxiv 2020
IMMUN
Plasmablast-derived antibody response to acute SARS-CoV-2 infection in humans
Kuan-Ying A. Huang…Alain R. Townsend. bioRxiv 2020.08.28.267526
IMMUN
The immunodominant and neutralization linear epitopes for SARS-CoV-2
Shuai Lu…Rui-tian Liu. bioRxiv Aug 2020 and Cell reports Jan 2021
IMMUN
Structural and functional analysis of LIM domain-dependent recruitment of paxillin to focal adhesions
Marta Ripamonti, Nicolas Liaudet, Bernhard Wehrle-Haller. Research Square preprint Aug 2020
MATERIALS
A Platform Incorporating Trimeric Antigens into Self-Assembling Nanoparticles Reveals SARS-CoV-2-Spike
Nanoparticles to Elicit Substantially Higher Neutralizing Responses than Spike Alone
Baoshan Zhang…Peter
D. Kwong. bioRxiv Aug 2020
IMMUN
DECORATED INCLUSION BODY AND USES THEREOF
WO/2020/165425 Publication Date 20.08.2020 ABERA BIOSCIENCE
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IMMUN
MATERIALS
A modular approach for dCas9-mediated enzyme cascading via orthogonal bioconjugation
Emily A Berckman and Wilfred Chen. Chem Comm Aug 2020
ENZ
Dodecin as carrier protein for immunizations and bioengineering applications
Florian Bourdeaux…Martin Grininger. Scientific Reports volume 10, Article number: 13297 (2020)
IMMUN
The Design of Vaccines Based on the Shielding of Antigenic Site Ø of a Respiratory Syncytial Virus Fusion Protein
Immunogen
Steven J. Frey…Ravi S. Kane. Adv Health Mater 05 August 2020
IMMUN
Detecting protein and post-translational modifications in single cells with iDentification and
qUantification sEparaTion (DUET)
Yandong Zhang…Long Cai. Communications Biology volume 3, Article number: 420 (2020)
Use of Specific Covalent Bonding for Oriented Immobilization of Recombinant Antibody
Fragments
US patent Publication Date 30.07.2020 Inventors George P. Anderson, Jinny Lin Liu, Ellen R.
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IMMUN
Cellular Synthesis and X-ray Crystal Structure of a Designed Protein Heterocatenane
Yajie Liu…Wen-Bin Zhang. Ang Chemie Jul 2020
MATERIALS
Calcium-Responsive Hydrogels Enabled by Inducible Protein-Protein Interactions
Jiren Luo and Fei Sun. Polymer Chemistry Jul 2020
MATERIALS
A Vaccine Displaying a Trimeric Influenza-A HA Stem Protein on Capsid-Like Particles Elicits Potent and Long-Lasting Protection in Mice
Susan Thrane…Adam F. Sander. Vaccines July 2020
IMMUN
GLYCO-ENGINEERED IMMUNIZATION ANTIGENS
Patent WO/2020/144358 16.07.2020 EXPRES2ION BIOTECHNOLOGIES APS [DK]
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Influence of Fluorination on Single-Molecule Unfolding and Rupture Pathways of a Mechanostable Protein Adhesion Complex
Byeongseon Yang…Michael A. Nash. bioRxiv July 2020
MATERIALS
Power to the Protein: Enhancing and Combining Activities using the Spy Toolbox
Anthony H. Keeble and Mark Howarth. Chemical Science July 2020 11, 7281 - 7291
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In vivo Characterization of Plasmodium berghei P47 (Pbs47) as a Malaria Transmission-Blocking Vaccine Target
Lampouguin Yenkoidiok-Douti…Carolina Barillas-Mury. Front. Microbiol., 03 July 2020
IMMUN
Lasso Proteins: Modular Design, Cellular Synthesis and Topological Transformation
Yajie Liu…Wenbin Zhang. Ang Chemie 30 June 2020
Programmable Modular Assembly of Functional Proteins on Raman-Encoded Zeolitic Imidazolate Framework-8 (ZIF-8) Nanoparticles as SERS Tags
Sarah De Marchi…Isabel Pastoriza-Santos. Chem. Mater. 2020, 32, 13, 5739–5749
MATERIALS
Post-translational knockdown and post-secretional modification of EsxA unambiguously determine the role of EsxA membrane permeabilizing
activity in mycobacterial virulence.
Yanqing Bao…Jianjun Sun. bioRxiv June 2020
Multi-Enzyme Assembly on T4 Phage Scaffold
Jinny L. Liu…Ellen R. Goldman. Front. Bioeng. Biotechnol., 24 June 2020
ENZ
Spy chemistry-enabled protein directional immobilization and protein purification
Zhanglin Lin…Yanrui Ye. Biotech & Bioeng June 2020
Conditional Protein Rescue (CPR) by Binding-Induced Protective Shielding
Andrew Scott Gaynor, Wilfred Chen. bioRxiv June 2020
Co-immobilization of multiple enzymes by self-assembly and chemical crosslinking for cofactor
regeneration and robust biocatalysis
Fei Peng…Wen-Yong Lou. International Journal of Biological Macromolecules. 18 June 2020
ENZ
A Versatile Platform to Incorporate Viral Trimeric Antigens into Self-Assembling Nanoparticle
Immunogens
Baoshan Zhang…Peter D. Kwong. bioRxiv 12 June 2020
IMMUN
Selective and Effective Cancer Treatments using Target-Switchable Intracellular Bacterial Toxin Delivery
Systems
Seong Guk Park…Sebyung Kang. Advanced Therapeutics 08 June 2020
IMMUN
Structural asymmetry does not indicate hemiphosphorylation in the bacterial histidine kinase CpxA
Sophie Bouillet1, Ti Wu, Shaoxing Chen, Ann M. Stock and Rong Gao. J Biol Chem June 2020.
ENZ
COMPOSITIONS AND METHODS FOR THE CYTOPLASMIC DELIVERY OF ANTIBODIES AND OTHER
PROTEINS
US patent WO/2020/102609 Publication Date 22.05.2020 Inventors TSOURKAS, Andrew
IMMUN
Particulate multivalent presentation of the receptor binding domain induces protective immune responses against MERS-CoV
Nisreen M. A. Okba…Bart L. Haagmans. Emerging Microbes & Infections 2020 May
IMMUN
NMR Spectroscopic Studies Reveal the Critical Role of Isopeptide Bond in Forming the Otherwise Unstable SpyTag-SpyCatcher
Mutant Complexes
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Basis of Specificity for a Conserved and Promiscuous Chromatin Remodeling Protein
Drake A Donovan…Jeffrey N McKnight. bioRxiv May 2020 and eLife Feb 2021
ENZ
HRP-conjugated plug-and-playable IgG-binding nanobodies as secondary antibody mimics in immunoassays
Yoonji Bae …Sebyung Kang. Sensors & Actuators 24 May 2020
ENZ
The relationship between effective molarity and affinity governs rate enhancements in tethered kinase-substrate reactions
Elizabeth B Speltz, and Jesse G. Zalatan. Biochemistry, 20 May 2020
ENZ
Conformable self-assembling amyloid protein coatings with genetically programmable functionality
Yingfeng Li…Chao Zhong. Science Advances 20 May 2020:Vol. 6, no. 21, eaba1425
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A novel rapid modularized hepatitis B Core virus-like particle-based platform for personalized Cancer vaccine preparation via fixed-
point coupling
Mei Ji…Rui-tian Liu. Nanomedicine: Nanotechnology, Biology and Medicine. 16 May 2020, 102223
IMMUN
Combining Protein Ligation Systems to Expand the Functionality of Semi-Synthetic Outer Membrane Vesicle Nanoparticles
H. Bart van den Berg van Saparoea…Wouter S. P. Jong. Frontiers Microbiol 12 May 2020
IMMUN
Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins
Laurens Lindenburg…Florian Hollfelder. Nucleic Acids Research, 08 May 2020
Column-free purification and coating of SpyCatcher protein on ELISA wells generates universal solid support for capturing of SpyTag-
fusion protein from the non-purified condition
Mohammad Rahmati-Yamchi…David Wood. Protein Expression and Purification, 29 April 2020, 105650
Structural Determination of a Filamentous Chaperone to Fabricate Electronically Conductive Metalloprotein Nanowires
Yun X…Dominic J. Glover. ACS Nano, 29 Apr 2020
MATERIALS
Native conjugation between proteins and [60]fullerene derivatives using SpyTag as a reactive handle
Guangzhong Yin…Wen-Bin Zhang. Chinese Chemical Letters 26 April 2020
MATERIALS
Enzymatic degradation of algal 1,3-xylan: from synergism of lytic polysaccharide monooxygenases with β-1,3-xylanases to their intelligent immobilization on
biomimetic silica nanoparticles
Lixi Cai…Guangya Zhang. Applied Microbiology and Biotechnology (Apr 2020)
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MATERIALS
Multimeric single-domain antibody complexes protect against bunyavirus infections
Paul J Wichgers Schreur …Andrea Aebischer. eLife Apr 21, 2020
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Pat1 increases the range of decay factors and RNA bound by the Lsm1-7 complex
Joseph H Lobel, John D Gross. bioRxiv Apr 10 2020
IMAGING
A self-adjuvanted, modular, antigenic VLP for rapid response to Influenza virus variability
Jhanvi Sharma…Trevor Douglas. ACS Appl. Mater. Interfaces 2020, April 1
IMMUN
Implementing Optogenetic Modulation in Mechanotransduction
Miao Yu…Jie Yan. Phys. Rev. X 10, 021001 – Published 1 April 2020
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Immunization with virus-like particles conjugated to CIDRα1 domain of Plasmodium falciparum erythrocyte membrane
protein 1 induces inhibitory antibodies.
Charlotte Harmsen…Thomas Lavstsen. Malaria J 19, 132 (2020)
IMMUN
Split chimeric antigen receptor-modified T cells targeting glypican-3 suppress hepatocellular carcinoma growth with
reduced cytokine release
Xuan Liu…Xiaotao Jiang. Ther Adv Med Oncol. 2020 Mar 9.
IMMUN
Multiplex single-molecule kinetics of nanopore-coupled polymerases
Mirkό Palla…George M. Church. bioRxiv 2020 Mar
SEQUENCING
HIGH-THROUGHPUT IDENTIFICATON OF INTERMOLECULAR INTERACTIONS
Patent WO/2020/061262 Publication Date 26.03.2020 GUTTMAN, Mitchell; US et al.
IMMUN
HIV-1 ENV FUSION PEPTIDE NANOPARTICLE CARRIER CONJUGATES AND THEIR USE
Patent Publication Number WO/2020/061564 Publication Date 26.03.2020, NIH; TSYBOVSKY, Yaroslav et al.
IMMUN
Engineering and functionalization of large circular tandem repeat protein nanoparticles
Colin E. Correnti…Barry L. Stoddard & Philip Bradley. Nature Structural & Molecular Biology (2020) Mar
IMMUN
CRISPR/Cas-directed programmable assembly of multi-enzyme complexes
Samuel Lim, Jiwoo Kim, Yujin Kim, Dawei Xu and Douglas S. Clark. Chem Comm Mar 2020
ENZ
Dodecin as carrier protein for immunizations and bioengineering applications
Florian Bourdeaux…Martin Grininger. bioRxiv Mar 2020
IMMUN
Quantitative Control of Gene-Engineered T cell Activity Through Covalent Attachment of Targeting Ligands to
a Universal Immune Receptor
Nicholas G. Minutolo…Andrew Tsourkas, Daniel J. Powell. J. Am. Chem. Soc. March 19, 2020
IMMUN
Surface Display of Complex Enzymes by In Situ SpyCatcher-SpyTag Interaction
Sabrina Gallus…Kersten S. Rabe. ChemBioChem17 March 2020
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The relationship between effective molarity and affinity governs rate enhancements in tethered kinase-
substrate reactions
Elizabeth B Speltz, Jesse G Zalatan. bioRxiv Mar 2020
ENZ
Structural asymmetry does not indicate hemi-phosphorylation in the bacterial histidine kinase CpxA
Sophie Bouillet1, Ti Wu1, Shaoxing Chen1, Ann M. Stock2 and Rong Gao3*. J Biol Chem, Feb 24 2020
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Adjustable Bioorthogonal Conjugation Platform for Protein Studies in Live Cells based on Artificial Compartments
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METHOD FOR PREPARING L-THREO/ERYTHRO-P-METHYLSULFONYLPHENYL SERINE AND ENZYME FOR METHOD
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Binding Induced Stabilization Measured on the Same Molecular Protein Substrate using Single Molecule Magnetic Tweezers and Hetero-Covalent
Attachments
Narayan Dahal…Ionel Popa. J. Phys. Chem. B February 25, 2020
MATERIALS
One-pot synthesis of heterodimeric agonists that activate the canonical Wnt signaling pathwa
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Abhirup Mukherjee…Ravi S. Kane. Chem Commun, Mar 2020
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Dual-targeting nanoparticle vaccine elicits a therapeutic antibody response against chronic hepatitis B
Wenjun Wang…Mingzhao Zhu. Nature Nanotechnology 2 Mar 2020
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GRAPHENE OXIDE AFFINITY SAMPLE GRIDS FOR CRYO-EM
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HEAT-STABLE ENTEROTOXINS MUTANTS AS ANTIDIARRHEAL VACCINE ANTIGENS
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LIQUID-LIQUID PHASE SEPARATION DRIVEN PROTEIN-BASED UNDERWATER ADHESIVE COATINGS
Patent WO/2020/038246 Publication Date 27.02.2020 ZHONG, Chao; CN; CUI, Mengkui; CN
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SpyTag/SpyCatcher cyclization and covalent immobilization in enhancing cephalosporin C acylase stability
Yue Wang… Zhongyao Shen. Process Biochemistry 24 February 2020
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Construction, characterization, and immunization of nanoparticles that display a diverse array of influenza HA trimers
Alexander A. Cohen…Pamela J. Bjorkman. bioRxiv Jan 2020 and PLoS One Mar 2021
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