HOWARTH LAB
SOCIAL
ADDRESS Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD UK
CONTACT e: mh2186@cam.ac.uk t: 01223 334176
2024 Mark Howarth. All rights reserved.
Papers and Patents using Spy technology Many of these publications don’t come up from a Pubmed search or citation search, so it should be useful to bring them all together and hyperlinked (now >900 publications). Patents may be omitted if there is a journal paper covering very similar work. Ones with particular relevance to IMMUNOLOGY, MATERIALS (polymers or mechanobiology), IMAGING , SEQUENCING or ENZYMES are marked. For an overview, please see our open-access review “Power to the Protein” from 2020. SpyBank is a database of >1,000 Tag/Catcher aa sequences to download (Excel file), from publications below. SpyBank includes includes expression organism, Tag/Catcher location (N-terminal, internal, C-terminal) and annotated linkers, as a reference for groups creating their own fusions. SpyBank itself is described in: “Insider information on successful covalent protein coupling with help from SpyBank. Keeble AH, Howarth M. Meth Enz 2019 PDF”. (See note on codon usage at Reagents page). Most recent below. Publications 301-600 here. Publications 1-300 here. SpyMask enables combinatorial assembly of bispecific binders Claudia L. Driscoll…Mark R. Howarth. Nature Communications volume 15, Article number: 2403 (2024) IMMUN Molecular density-accelerated binding-site maturation underlies CENP-T-dependent kinetochore assembly Ekaterina V. Tarasovetc…Ekaterina L. Grishchuk. bioRxiv 2024 Rational structure-guided design of a blood stage malaria vaccine immunogen presenting a single epitope from PfRH5 Thomas E. Harrison…Matthew K. Higgins. bioRxiv 2024 IMMUN Designed mosaic nanoparticles enhance cross-reactive immune responses in mice Eric Wang…Arup K. Chakraborty. bioRxiv 2024 IMMUN Architecture and flexibility of native kinetochores revealed by structural studies utilizing a thermophilic yeast Daniel J. Barrero…Sue Biggins. bioRxiv 2024 METHODS OF PRODUCING EXTRACELLULAR VESICLES Patent Application Number 18246304 Publication Date 14.03.2024 Applicants LONZA SALES AG Nanoparticle vaccines based on the receptor binding domain of porcine deltacoronavirus elicit robust protective immune responses in mice Yuanhong Wang…Lingxue Yu. Front. Immunol., 14 March 2024 IMMUN A cVLP-Based Vaccine Displaying Full-Length PCSK9 Elicits a Higher Reduction in Plasma PCSK9 Than Similar Peptide-Based cVLP Vaccines Louise Goksøyr…Adam F. Sander. Vaccines 2023, 11(1), 2; https://doi.org/10.3390/vaccines11010002 IMMUN NANOPARTICLE-DERIVED VACCINES AGAINST POXVIRUSES, AND METHODS FOR MAKING AND USING THE SAME Patent WO/2024/049990 Publication Date 07.03.2024 Inventors MOSS, Bernard et al. IMMUN Rapid and Convenient Single-Chain Variable Fragment-Employed Electrochemical C-Reactive Protein Detection System Daimei Miura… Ryutaro Asano. Int. J. Mol. Sci. 2024, 25(5), 2859 Site-directed immobilization of enzymes on nanoparticles using self-assembly systems Haili Zhou…Fei Peng. Bioresour Technol 2024 Feb 28:397:130505. ENZ MagIC-Cryo-EM: Structural determination on magnetic beads for scarce macromolecules in heterogeneous samples Yasuhiro Arimura…Hironori Funabiki. bioRxiv 2024 Feb HIGH THROUGHPUT PEPTIDE IDENTIFICATION USING CONJUGATED BINDERS AND KINETIC ENCODING US patent 18466543 Publication Date 15.02.2024 Applicants Encodia, Inc. SEQUENCIN Denaturing purifications demonstrate that PRC2 and other widely reported chromatin proteins do not appear to bind directly to RNA in vivo Jimmy K. Guo…Mitchell Guttman. Molecular Cell 21 February 2024 Mosaic sarbecovirus vaccination elicits cross-reactive responses in pre-immunized animals Alexander A. Cohen…Pamela J. Bjorkman. bioRxiv 2024 Feb IMMUN 975 LENGTH-ADAPTED CHIMERIC ANTIGEN RECEPTORS AND LENGTH-ADAPTED ACCESSORY RECEPTORS FOR IMPROVED CAR T CELL ACTIVATION Patent WO/2024/033642 Publication Date 15.02.2024 DUSHEK, Omer IMMUN Development and Immunological Evaluation of a Multiantigen Thermostable Nanovaccine Adjuvanted with T-Cell-Activating Scaffold for African Swine Fever Lidan Sun…Zeliang Chen. ACS Appl. Bio Mater. 2024 Feb IMMUN Irreversible light-activated SpyLigation mediates split-protein assembly in 4D Brizzia G. Munoz-Robles & Cole A. DeForest. Nature Protocols 2024 Jan MATERIALS OptoREACT: Optogenetic Receptor Activation on Nonengineered Human T Cells Anja Armbruster…Maximilian Hörner. ACS Synth. Biol. 2024 Feb IMMUN High-Level Secretory Production of Recombinant E2-Spy Antigen Protein via Combined Strategy in Pichia pastoris Bingkun Li…Ding Li. Fermentation 2024 Feb IMMUN Flow-Induced Microfluidic Assembly for Advanced Biocatalysis Materials Phillip Lemke…Christof M. Niemeyer. Advanced Functional Materials 24 January 2024 MATERIALS ENZ Nanoengineering Carboxysome Shells for Protein Cages with Programmable Cargo Targeting Tianpei Li…Lu-Ning Liu. ACS Nano 2024 Feb MATERIALS ENZ Active Template Synthesis of Protein [n]Catenanes Using Engineered Peptide–Peptide Ligation Tools Fan Zhang…Wen-Bin Zhang. CCS Chemistry Feb 2024 MATERIALS Development of plug-and-deliverable intracellular protein delivery platforms based on botulinum neurotoxin Seong Guk Park… Sebyung Kang. Int J Biol Macromol 2024 Jan 23;261(Pt 1):129622. ENZ Mechanically Tunable, Compostable, Healable and Scalable Engineered Living Materials Avinash Manjula-Basavanna…Anna Duraj-Thatte. bioRxiv 2024 Feb MATERIALS VIRAL PARTICLES RETARGETED TO TRANSFERRIN RECEPTOR 1 Patent WO/2024/026494 Publication Date 01.02.2024 REGENERON PHARMACEUTICALS, INC. [US]/[US] IMMUN De novo design of modular protein hydrogels with programmable intra- and extracellular viscoelasticity Rubul Mout…David Baker. PNAS 2024 Jan MATERIALS Exploration and Application of DNA-Binding Proteins to Make a Versatile DNA–Protein Covalent-Linking Patch (D-Pclip): The Case of a Biosensing Element Erika Komiya…Ryutaro Asano. J. Am. Chem. Soc. 2024 Jan Enhancing antibody responses by multivalent antigen display on thymus-independent DNA origami scaffolds Eike-Christian Wamhoff…Mark Bathe. Nature Communications volume 15, Article number: 795 (2024) MATERIALS IMMUN Spontaneous and site-specific immobilization of PNGase F via spy chemistry Liang Zhang…Song Wang. RSC Advances 2024 Jan ENZ Cryo-EM analysis on magnetic beads for scarce macromolecules in heterogeneous samples Yasuhiro Arimura…Hironori Funabiki. bioRxiv 2024 Jan MATERIALS Engineering multivalent Fc display for FcγR blockade Ekaterina Petrova…Ana I. Teixeira. bioRxiv 2024 Jan IMMUN Discovery and engineering of the antibody response against a prominent skin commensal Djenet Bousbaine…Michael A. Fischbach. bioRxiv 2024 Jan IMMUN Enhancing humoral and mucosal immune response of PED vaccine candidate by fusing S1 protein to nanoparticle multimerization Minghui Li…Gaiping Zhang. Veterinary Microbiology19 January 2024, 110003 IMMUN Photoactivatable Nanobody Conjugate Dimerizer Temporally Resolves Tiam1-Rac1 Signaling Axis Chengjian Zhou…Xi Chen. Advanced Science. 15 January 2024 OMPG VARIANTS Patent Application Number 18449904 Publication Date 11.01.2024 Applicants Roche Sequencing Solutions, Inc. SEQUENCING GENETICALLY ENGINEERED RECOMBINANT PROTEINS FOR FUNCTIONAL REGENERATIVE TISSUE SCAFFOLDS Patent Application Number 18365648 Publication Date 11.01.2024 Inventors Minkyu Kim et al. MATERIALS MOLECULAR PEPTIDE MUTANT Patent Application Number 18255108 Publication Date 04.01.2024 Applicants NANJING TECH UNIVERSITY Potent immune responses against thermostable Foot-and-Mouth disease virus VP1 nanovaccine adjuvanted with polymeric thermostable scaffold Yuanli Peng…Zeliang Chen. Vaccine 13 January 2024 IMMUN Affinity-assisted covalent self-assembly of PduQ-SpyTag and Nox-SpyCatcher to construct multi-enzyme complexes on the surface of magnetic microsphere modified with chelated Ni2+ Mianxing Luo…Guo Chen. International Journal of Biological Macromolecules 11 January 2024 ENZ 950 Light-based juxtacrine signaling between synthetic cells Hossein Moghimianavval…Allen P Liu. bioRxiv 2024 Jan Reengineering of an artificial protein cage for efficient packaging of active enzymes Yusuke Azuma…Jonathan G. Heddle. bioRxiv 2023 Dec ENZ Enhancing immune responses of ESC-based TAA cancer vaccines with a novel OMV delivery system Meiling Jin…Jian-Dong Huang. Journal of Nanobiotechnology volume 22, Article number: 15 (2024) IMMUN Hidden route of protein damage through confined oxygen gas Seoyoon Kim…Duyoung Min. bioRxiv 2024 Jan MATERIALS Preclinical Development of a Stabilized RH5 Virus-Like Particle Vaccine that Induces Improved Anti-Malarial Antibodies Lloyd D W King…Mark Howarth, Sumi Biswas, Simon J Draper. bioRxiv 2024 Jan IMMUN Cleavage-intermediate Lassa virus trimer elicits neutralizing responses, identifies neutralizing nanobodies, and reveals an apex-situated site-of-vulnerability Jason Gorman…Peter D. Kwong. Nature Communications volume 15, Article number: 285 (2024) IMMUN KARI NANOPARTICLE Patent WO/2024/006532 Publication Date 04.01.2024 OLMEDILLAS, Eduardo et al. Extraordinary Titer and Broad Anti-SARS-CoV-2 Neutralization Induced by Stabilized RBD Nanoparticles from Strain BA.5 Z. Wang…S. Wang. Vaccines 2024 IMMUN SpyDirect: A Novel Biofunctionalization Method for High Stability and Longevity of Electronic Biosensors Keying Guo…Sahika Inal. Advanced Science 31 December 2023 MATERIALS Display of PETase on the Cell Surface of Escherichia coli Using the Anchor Protein PgsA Takuma Yamashita…Hiroyasu Ogino. Applied Biochemistry and Biotechnology Jan 2024 ENZ Lipid nanodisc scaffold and size alter the structure of a pentameric ligand-gated ion channel Vikram Dalal…Wayland W. L. Cheng. Nature Communications volume 15, Article number: 25 (2024) Molecular Engineering of a Spider Silk and Mussel Foot Hybrid Protein Gives a Strong and Tough Biomimetic Adhesive Yin Yin…Markus B. Linder. Advanced Materials Interfaces 02 January 2024 MATERIALS METHODS FOR SPORE DISPLAY Patent WO/2023/245018 Publication Date 21.12.2023 Inventors NICKS, Trevor et al. MATERIALS IMMUN The SpyCatcher-SpyTag interaction mediates tunable anti-tumor cytotoxicity of NK cells. Guo C…Zhu W. Mol Immunol. 2023 Dec 20;165:11-18. IMMUN Ligand-Mediated Mechanical Enhancement in Protein Complexes at Nano- and Macro-Scale Samuel Kim…Minkyu Kim. ACS Appl. Mater. Interfaces 2023 MATERIALS De novo Semi-synthetic Platform for Monitoring Protein degradation in Live Cells Mahdi Hasan…Ashraf Brik. Chembiochem 2023 Nov 30:e202300731. Protein cage nanoparticle-based NK cell-engaging nanodrones (NKeNDs) effectively recruit NK cells to target tumor sites and suppress tumor growth Seong Guk Park…Sebyung Kang. Nanotoday 2024 Feb IMMUN Engineered immunogens to elicit antibodies against conserved coronavirus epitopes A. Brenda Kapingidza…Mihai L. Azoitei. Nature Communications volume 14, Article number: 7897 (2023) IMMUN METHODS AND COMPOSITIONS FOR DETERMINING THE ANTIGEN SPECIFICITY OF T CELLS Patent WO/2023/235761 Publication Date 07.12.2023 Inventors HACOHEN, Nir BAKALAR, Matthew IMMUN UNIVERSAL ADJUVANT FOR NASAL, ORAL, AND INTRAMUSCULAR DELIVERY OF VACCINES Patent WO/2023/230241 Publication Date 30.11.2023 DESIGN-ZYME LLC [US]/[US] IMMUN Encapsulation of AAV into protein vault nanoparticles as a novel solution to gene therapy's neutralizing antibody problem Logan Thrasher Collins…David Curiel. bioRxiv 2023 Nov IMMUN MATERIALS Accelerating the design of pili-enabled living materials using an integrative technological workflow Yuanyuan Huang…Chao Zhong. Nature Chemical Biology (2023) MATERIALS Enhancing anti-viral neutralization response to immunization with HIV-1 envelope glycoprotein immunogens Shamim Ahmed…Alon Herschhorn. npj Vaccines volume 8, Article number: 181 (2023) IMMUN Free Energy Landscape of Type III Fibronectin Domain with Identified Intermediate State and Hierarchical Symmetry Hao Sun…Hu Chen. Phys. Rev. Lett. 131, 218402 – Published 22 November 2023 MATERIALS Dimer stabilization by SpyTag/SpyCatcher coupling of the reductase domains of a chimeric P450 BM3 monooxygenase from Bacillus spp. improves its stability, activity and purification Arabella Essert, Kathrin Castiglione. ChemBioChem 2023 Nov ENZ 925 A candidate nanoparticle vaccine comprised of multiple epitopes of the African swine fever virus elicits a robust immune response Jinxing Song…Gaiping Zhang. Journal of Nanobiotechnology volume 21, Article number: 424 (2023) IMMUN VECTORS AND METHODS FOR IN VIVO ANTIBODY PRODUCTION Patent WO/2023/220603 Publication Date 16.11.2023 REGENERON PHARMACEUTICALS, INC. [US]/[US] IMMUN Study on the properties of a dual-system-based protein scaffold for orthogonal self-assembly Meng Zhang…Jun Zhao. International Journal of Biological Macromolecules16 November 2023, 127946 MATERIALS Development of a Tag/Catcher-mediated capsid virus-like particle vaccine presenting the conserved Neisseria gonorrhoeae SliC antigen that blocks human lysozyme Fabian G Martinez…Aleksandra E Sikora. Infect Immun 2023 Nov 2:e0024523. IMMUN Protein nanoscaffold enables programmable nanobody-luciferase immunoassembly for sensitive and simultaneous detection of aflatoxin B1 and ochratoxin A Shaowen Wu…Shijuan Yan. J Hazard Mater 2023 Oct 5:462:132701. ENZ A novel protein purification scheme based on salt inducible self-assembling peptides Guang Zeng…Zhanglin Lin. Microbial Cell Factories volume 22, Article number: 224 (2023) A BACTERIOPHAGE-BASED, NEEDLE AND ADJUVANT-FREE, MUCOSAL COVID-19 VACCINE Patent WO/2023/209556 Publication Date 02.11.2023 Inventors RAO, Venigalla B. et al. IMMUN VARIANTS OF THE GAIN DOMAIN OF ADHESION G PROTEIN-COUPLED RECEPTORS Patent WO/2023/213993 Publication Date 09.11.2023 Inventors MARFOGLIA, Matthieu et al. METHOD FOR BIOSYNTHESIS OF PROTEIN HETEROCATENANE Patent United States of America Application Number 17999377 Publication Date 02.11.2023 Inventors Wenbin ZHANG et al MATERIALS A modular approach to enhancing cell membrane-coated nanoparticle functionality using genetic engineering Nishta Krishnan…Liangfang Zhang. Nature Nanotechnology (2023) Oct IMMUN A nanoparticle vaccine displaying the ookinete PSOP25 antigen elicits transmission-blocking antibody response against Plasmodium berghei Guixiang Yao…Yaming Cao. Parasites & Vectors volume 16, Article number: 403 (2023) IMMUN Molecular imaging of bacterial outer membrane vesicles based on bacterial surface display Dávid Szöllősi…Krisztián Szigeti. Scientific Reports volume 13, Article number: 18752 (2023) CONJUGATE SYSTEMS AND METHODS OF USE THEREOF Patent Application Number 18301228 Publication Date 19.10.2023 Applicants David Curiel et al. IMMUN MODIFIED VIRAL PARTICLES FOR GENE THERAPY US patent Application Date 11.05.2023 Publication Date 26.10.2023 Inventors Paul Alexander HEPPENSTALL IMMUN LONG LIFETIME ALPHA-HEMOLYSIN NANOPORES US patent Application Number 18171969 Publication Date 19.10.2023 Applicants Roche Sequencing Solutions SEQUENCING Engineered Reversible Inhibition of SpyCatcher Reactivity Enables Rapid Generation of Bispecific Antibodies Christian Hentrich…Francisco Ylera. bioRxiv 2023 Oct IMMUN Mechanical disengagement of the cohesin ring Martina Richeldi…Maxim I. Molodtsov. Nature Structural & Molecular Biology (2023) Oct MATERIALS Thermostable T Cell Multiepitope Nanoparticle Antigens Inducing Potent Immune Responses against the Swine Fever Virus Lidan Sun…Zeliang Chen. ACS Infect. Dis. 2023, Oct IMMUN Toward Minimal Transcription–Translation Machinery Ting Wang and Yuan Lu. ACS Synth. Biol. 2023 Oct ENZ An Antibiotic Degrading Engineered Living Material Platform to Combat Environmental Antibiotic Resistance Gokce Ozkul…Ozgur Safak Seker. bioRxiv 2023 Oct MATERIALS HIGH THROUGHPUT PEPTIDE IDENTIFICATION USING CONJUGATED BINDERS AND KINETIC ENCODING Patent WO/2023/201365 Publication Date 19.10.2023 ENCODIA, INC. [US]/[US] SEQUENCING Engineering of an Optogenetic T Cell Receptor Compatible with Fluorescence-Based Readouts Vincent Idstein…Wolfgang W. Schamel. ACS Synth. Biol. 2023 Oct IMMUN Biological Applications of Synthetic Binders Isolated from a Conceptually New Adhiron Library Claudia D’Ercole…Ario de Marco. Biomolecules 2023, 13(10), 1533 Synthetic Par polarity induces cytoskeleton asymmetry in unpolarized mammalian cells JL Watson…E Derivery. Cell Volume 186, Issue 21, 12 October 2023, Pages 4710-4727.e35 Unveiling Mechanical Activation: GAIN Domain Unfolding and Dissociation in Adhesion GPCRs Chaoyu Fu…Jie Yan. Nano Lett. 2023 Oct MATERIALS 900 Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation Jiayu Liu…Jing Zhou. Nature Communications volume 14, Article number: 6457 (2023) MATERIALS Living Magnetotactic Microrobots Based on Bacteria with a Surface-Displayed CRISPR/Cas12a System for Penaeus Viruses Detection Haoxiang Chen…Lei Ren. ACS Appl. Mater. Interfaces 2023 Oct MATERIALS Accurate location of two conserved linear epitopes of PEDV utilizing monoclonal antibodies induced by S1 protein nanoparticles Minghui Li…Gaiping Zhang. Int J Biol Macromol 2023 Oct 5:127276. IMMUN MicroRNAs are necessary for the emergence of Purkinje cell identity Norjin Zolboot…Giordano Lippi. bioRxiv 2023 September BACTERIAL MICROCOMPARTMENT VIRUS-LIKE PARTICLES Patent Application Number 18033282 Publication Date 28.09.2023 Inventors Yong Quan Tan et al. GROWTH AND SITE-SPECIFIC ORGANIZATION OF MICRO-SCALE BIMOLECULAR DEVICES ON LIVING CELLS Patent Application Number 18003462 Publication Date 28.09.2023 Inventors Rebecca B. Schulman et al. Hypoallergenic chimeric virus-like particles for the development of shrimp tropomyosin allergen Pen m 1-specific blocking antibodies Supapich Chanasit…Alain Jacquet. Allergy 2023 September IMMUN Native Agarose Gels and Contact Blotting as Means to Optimize the Protocols for the Formation of Antigen–Ligand Complexes Claudia D’Ercole and Ario de Marco. Bioengineering 2023 September Two molecule force spectroscopy on ligand–receptor interactions Jiacheng Zuo…Hongbin Li. Nanoscale 2023 September MATERIALS A SARS-CoV-2 nanoparticle vaccine based on chemical conjugation of loxoribine and SpyCatcher/SpyTag Wenying Yan…Tao Hu. Int J Biol Macromol 2023 Sep 29;127159. IMMUN Ligand requirements for immunoreceptor triggering Michael I. Barton…Anton van der Merwe. bioRxiv 2023 Sept IMMUN Engineered Immunogens to Elicit Antibodies Against Conserved Coronavirus Epitopes Brenda Kapingidza…Mihai L. Azoitei. bioRxiv 2023 September IMMUN Bioluminescent detection of viral surface proteins using branched multivalent protein switches Alexander Grawe…Maarten Merkx. bioRxiv 2023 Sept IMMUN MATERIALS Engineered Escherichia coli Consortia Function in a Programmable Pattern for Multiple Enzymatic Biosynthesis Wenxue Zhang…Ping Wang. ACS Appl. Mater. Interfaces 2023 September ENZ Recording Binding Information Directly into DNA-Encoded Libraries Using Terminal Deoxynucleotidyl Transferase Lukas A. Schneider…Dennis Gillingham. JACS 2023 September ENZ Snoopligase-catalyzed molecular glue enables efficient generation of hyperoligomerized TRAIL variant with enhanced antitumor effect T She…X Lu. Journal of Controlled Release. September 2023, Pages 856-870 MATERIALS Molecular basis of product recognition during PIP5K-mediated production of PI(4,5)P2 with positive feedback Benjamin R. Duewell…Scott D. Hansen. bioRxiv 2023 September ENZ The molecular reach of antibodies determines their SARS-CoV-2 neutralisation potency Anna Huhn…Omer Dushek. bioRxiv 2023 Sept IMMUN AP profiling resolves co-translational folding pathway and chaperone interactions in vivo Xiuqi Chen…Christian M. Kaiser. bioRxiv 2023 September In-situ Single-Molecule Investigations of the Impacts of Biochemical Perturbations on Conformational Intermediates of Monomeric α-Synuclein Wenmao Huang…Jie Yan. bioRiv 2023 Sept MATERIALS HIV-1 binds dynein directly to hijack microtubule transport machinery Somayesadat Badieyan…Michael A. Cianfrocco. bioRxiv 2023 Sept MATERIALS A platform for dissecting force sensitivity and multivalency in actin networks Joseph T. Levin…Gregory M. Alushin. bioRxiv 2023 Aug MATERIALS Rationally designed multimeric nanovaccines using icosahedral DNA origami for molecularly controlled display of SARS-CoV-2 receptor binding domain Qingqing Feng…Xiao Zhao. bioRiv 2023 Aug IMMUN MATERIALS Engineering Protein Nanoparticles Functionalized with an Immunodominant Coxiella burnetii Antigen to Generate a Q Fever Vaccine Aaron Ramirez…Szu-Wen Wang. Bioconjugate Chem. 2023 September IMMUN Compact and modular bioprobe: Integrating SpyCatcher/SpyTag recombinant proteins with zwitterionic polymer-coated quantum dots Sunghwan Kim…Jongnam Park. J Colloid Interface Sci 2023 Aug 6;652(Pt A):184-194. 875 SpyCombinator Assembly of Bispecific Binders Claudia L Driscoll…Mark Howarth. bioRxiv 2023 Sept Neoantigen-Based Nanovaccine In Combination with Immune Checkpoint Inhibitors Abolish Postsurgical Tumor Recurrence and Metastasis Peng Zheng…Yanbing Ma. Small 2023 Aug IMMUN GENETICALLY CONTROLLED NANOSCOPY CONTRAST-GENERATING UNITS, GENETICALLY CONTROLLED STRUCTURAL ELEMENTS, GENETICALLY CONTROLLED SCAFFOLDS, NANOBIOMATERIAL BASED THEREON, AND USE THEREOF IN NANOSOCOPY METHODS Patent WO/2023/156638 Publication Date 24.08.2023 Inventors WESTMEYER, Gil Gregor et al. MATERIALS IMAGING METHODS AND COMPOSITIONS FOR TREATING OR AMELIORATING CARDIAC MUSCLE ARRHYTHMIAS AND SKELETAL MUSCLE TREMORS Patent WO/2023/159234 Publication Date 24.08.2023 Inventors HARRIS, Samantha, P. MATERIALS A platform for dissecting force sensitivity and multivalency in actin networks Joseph T. Levin…Gregory M. Alushin. bioRxiv 2023.08.15.553463 MATERIALS Myddosome clustering in IL-1 receptor signaling regulates the formation of an NF-kB activating signalosome Fakun Cao…Marcus J Taylor. EMBO Reports (2023)e57233 IMMUN Affinity-induced covalent protein-protein ligation via the SpyCatcher-SpyTag interaction Jacob O. Fierer…Edward A. Bayer. Green Carbon 17 July 2023 ENZ Visible light-induced specific protein reaction delineates early stages of cell adhesion Rolle Rahikainen…Mark Howarth. J. Am. Chem. Soc. 2023 Oct and bioRxiv 2023.07.21.549850 MATERIALS IMAGING ANTIBODIES AND ANTIBODY FRAGMENTS AND ANALOGUES SPECIFIC FOR CHONDROITIN SULFATE Patent WO/2023/148398 Publication Date 10.08.2023 Applicants VAR2 PHARMACEUTICALS APS [DK]/[DK] IMMUN ANTIBODIES FOR CORONAVIRUS AND USES THEREOF Patent US Application Number 18060814 Publication Date 10.08.2023 Pamela J. Bjorkman et al. IMMUN METHODS FOR GENERATING NUCLEIC ACID ENCODED PROTEIN LIBRARIES AND USES THEREOF Patent WO/2023/150742 Publication Date 10.08.2023 Inventors ABATE, Adam et al. Self-assembled multienzyme complex facilitates synthesis of glucosylglycerol from maltodextrin and glycerol Xinming Sun…Yuanxia Sun. J Sci Food Agric 2023 Aug 8. ENZ Harnessing split fluorescent proteins in modular protein logic for advanced whole-cell detection Ping-Hen Lim…Yi-Chun Yeh. Analytica Chimica Acta 1275, 22 Sept 2023, 341593 IMAGING Thermostability enhancement of polyethylene terephthalate degrading PETase using self- and nonself-ligating protein scaffolding approaches Barindra Sana…John F Ghadessy. Biotechnol Bioeng 2023 Aug 9. ENZ Toward Selective Synthesis of Protein Olympiadanes via Orthogonal Active Templates in One Step Fan Zhang…Wen-Bin Zhang. CCCS Chem 2023 Jul MATERIALS KITS FOR ANALYSIS USING NUCLEIC ACID ENCODING AND/OR LABEL US patent Application Number 18166461, Application Date 08.02.2023. Encodia, Inc. Nanoparticle-based multivalent human antibodies offer potent and broad neutralization against Omicron sublineages Yizhen Wang…Ningshao Xia. Signal Transduction and Targeted Therapy volume 8: 284 (2023) IMMUN Raman Spectroscopy Combined with Malaria Protein for Early Capture and Recognition of Broad-Spectrum Circulating Tumor Cells Xinning Liu…Jinbo Yang. Int. J. Mol. Sci. 2023, 24(15), 12072 IMAGING In vivo fate of systemically administered encapsulin protein nanocages and implications for their use in targeted drug delivery Claire Rennie…Andrew Care. bioRxiv 2023 July MATERIALS Truly tiny acoustic biomolecules for ultrasound imaging and therapy Bill Ling…Mikhail G. Shapiro. bioRxiv 2023 June MATERIALS IMAGING Cell Cycle Dependent Orchestration of Surface Layer Biogenesis in Caulobacter crescentus Matthew Herdman…Tanmay A.M. Bharat. bioRxiv 2023 June MATERIALS De novo design of modular protein hydrogels with programmable intra- and extracellular viscoelasticity Rubul Mout…David Baker. bioRxiv 2023 June MATERIALS POLYPEPTIDE TERMINAL BINDERS AND USES THEREOF US Patent Application Number 17539033 Publication Date 13.07.2023 Encodia, Inc. ENZ SEQUENCING MULTIPLEXED IMMUNOSIGNAL AMPLIFICATION USING HYBRIDIZATION CHAIN REACTION-BASED METHOD US Patent Application Number 18045811 Publication Date 13.07.2023 Rui LIN Minmin LUO ENZ Construction of metal–organic framework-based multienzyme system for l-tert- leucine production Ru Wang…Feng Xue. Bioprocess and Biosystems Engineering 2023 July MATERIALS ENZ Bacterial Surface-Assembled Chitinosome for Dismantling Chitin into N-Acetyl Glucosamine Chao Du…Guimin Zhang. ACS Sustainable Chem. Eng. 2023 July MATERIALS ENZ 850 CATENATED VAR2CSA RECOMBINANT PROTEIN, PREPARATION METHOD THEREFOR, AND USE THEREOF Patent WO/2023/130667 Publication Date 13.07.2023 CAS LAMVAC (GUANGZHOU) BIOMEDICAL TECHNOLOGY CO., LTD [CN]/[CN] IMMUN Long-term retention of antigens in germinal centers is controlled by the spatial organization of the follicular dendritic cell network Ana Martínez-Riaño…Pavel Tolar. Nature Immunology (2023 July) IMMUN Malaria Biomimetic for Tumor Targeted Drug Delivery Jessica Pihl…Ali Salanti. ACS Nano July 12, 2023 IMMUN Cooperation between bHLH transcription factors and histones for DNA access Alicia K. Michael…Nicolas H. Thomä. Nature 2023 Jul Simultaneous Mapping of DNA Binding and Nucleosome Positioning with SpLiT-ChEC Orion G. B. Banks…Laura E. McKnight. bioRxiv Jul 2023 ENZ METHOD FOR PRODUCING VIRUS-FILTERING MODIFIED LECTIN-BINDING MODIFIED BIOFILM Patent WO/2023/128981 Publication Date 06.07.2023 BILKENT UNIVERSITESI Inventors SEKER, Urartu Ozgur Safak et al. MATERIALS The position of Spy Tag/Catcher system in hepatitis B core protein particles affects the immunogenicity and stability of the synthetic vaccine Y Sheng…S Zhang. Vaccine 28 June 2023 IMMUN T Cell Activating Thermostable Self-Assembly Nanoscaffold Tailored for Cellular Immunity Antigen Delivery Jinsong Zhang…Zeliang Chen. Advanced Science 3 July 2023 MATERIALS IMMUN IMMOBILISED ENZYME Patent WO/2023/118901 Publication Date 29.06.2023 FABRICNANO LIMITED [GB]/[GB] MATERIALS ENZ METHODS FOR BALANCING ENCODING SIGNALS OF ANALYTES Patent WO/2023/122698 Publication Date 29.06.2023 ENCODIA, INC. [US]/[US] MATERIALS ENZ SEQUENCING Biocatalytic Foams from Microdroplet-Formulated Self-Assembling Enzymes Julian S Hertel…Christof M Niemeyer. Adv Mater 2023 Jun 26;e2303952. MATERIALS ENZ Bioinspired Amyloid Fibril-Based Hydrogel with Engineering Programable Functionalities for Diverse Applications Weiqiang Wang…Honghua Ge. Advanced Functional Materials 26 June 2023 MATERIALS An RBD virus-like particle vaccine for SARS-CoV-2 induces cross-variant antibody responses in mice and macaques Yuanyuan Li…Jing Jin. Signal Transduction and Targeted Therapy volume 8, Article number: 173 (2023) IMMUN Self-assembled ferritin nanoparticles displaying PcrV and OprI as an adjuvant-free Pseudomonas aeruginosa vaccine Yuhang Li…Jiang Gu. Front. Immunol., 21 June 2023 IMMUN Sneaking in SpyCatcher using cell penetrating peptides for in vivo imaging James Tyler…Behzad Rad. Nanotechnology 2023 June IMAGING Using CombiCells, a platform enabling titration and combinatorial display of cell surface ligands, to investigate the sensitivity and costimulatory requirements of TCRs and CARs Ashna Patel…Omer Dushek. EMBO J 2023 Dec and bioRxiv 2023 Jun IMMUN Designed Multifunctional Spider Silk Enabled by Genetically Encoded Click Chemistry Bojing Jiang…Fei Sun. Advanced Functional Materials 15 June 2023 MATERIALS Preclinical immunogenicity and protective efficacy of a SARS-CoV-2 RBD-based vaccine produced with the thermophilic filamentous fungal expression system Thermothelomyces heterothallica C1 Mariana Gonzalez-Hernandez…Albert D. M. E. Osterhaus. Front. Immunol., 09 June 2023 IMMUN Modification of Bacterial Microcompartments with Target Biomolecules via Post-Translational SpyTagging David Mark Beal…Martin Warren. Mater. Adv., 2023 June MATERIALS SWITCHABLE POLYPEPTIDE AND ITS USE FOR GENTLE AFFINITY PURIFICATION Patent WO/2023/099876 Publication Date 08.06.2023 HOWARTH, Mark, ANUAR, Irsyad Khairil, RAHIKAINEN, Rolle ANTIBODIES FOR CORONAVIRUSES AND USES THEREOF Patent WO/2023/102476 Publication Date 08.06.2023 BJORKMAN, Pamela J. et al. 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Food Chem Sept 2022 ENZ Long-term retention of antigens in germinal centres is controlled by the spatial organisation of the follicular dendritic cell network Ana Martinez-Riano…Pavel Tolar, bioRxiv Sept 2022 IMMUN CD4-binding site immunogens elicit heterologous anti-HIV-1 neutralizing antibodies in transgenic and wildtype animals Harry B Gristick…Pamela J Bjorkman. bioRxiv Sept 2022 IMMUN Mechanical regulation of Titin N2B-us conformation and its binding to FHL2 Yuze Sun…Jie Yan. bioRxiv Sept 2022 MATERIALS 675 AN ARTIFICIAL PROTEIN-CAGE DECORATED WITH PARTICULAR MOLECULES ON THE EXTERIOR Patent WO/2022/182260 Publication Date 01.09.2022 HEDDLE, Jonathan et al. MATERIALS AN ARTIFICIAL PROTEIN-CAGE COMPRISING ENCAPSULATED THEREIN A GUEST CARGO Patent WO/2022/182262 Publication Date 01.09.2022 HEDDLE, Jonathan et al. 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Chem Eur J Aug 2022 MATERIALS ENZ ADHESIVE PROTEIN Patent Application Number 17628132 Publication Date 18.08.2022 Katsutoshi Hori MATERIALS Single-step purification of functionalized protein nanostructures using multimodal chromatography Daniel L. Winter…Dominic J. Glover. J Bionanotechnology 2023 and bioRxiv MATERIALS Passive immunization with equine RBD-specific Fab protects K18-hACE2-mice against Alpha or Beta variants of SARS-CoV-2 Mariette Barbier…F. Heath Damron. Front Imm Aug 2022 IMMUN RBD-VLP Vaccines Adjuvanted with Alum or SWE Protect K18-hACE2 Mice against SARS-CoV-2 VOC Challenge Ting Y. Wong…F. Heath Damron. mSphere Aug 2022 IMMUN SPECIFIC SELECTION OF IMMUNE CELLS USING VERSATILE DISPLAY SCAFFOLDS US patent Application Number 17614711 Publication Date 04.08.2022 Scott Eugene LINDNER et al. IMMUN MODIFIED VIRAL PARTICLES AND USES THEREOF Patent Application Number 17612669 Publication Date 04.08.2022 Regeneron Pharmaceuticals, Inc. Valence-controlled protein conjugation on nanoparticles via re-arrangeable multivalent interactions of tandem repeat protein chains Hyeongjoo Choi, Yongwon Jung. Chem Sci Aug 2020 MATERIALS A "Plug-and-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-binding Domain Rang Feng…Hai-Bo Li. J Vis Exp 2022 Jul 25 IMMUN Protein Cages Engineered for Interaction-Driven Selective Encapsulation of Biomolecules Yeolin Lee…Yongwon Jung. ACS Appl. Mater. Interfaces Aug 2022 MATERIALS Interactions between mTORC2 core subunits Rictor and mSin1 dictate selective and context-dependent phosphorylation of substrate kinases SGK1 and Akt. Zanlin Yu…David Pearce. J Biol Chem Aug 2022 Bacterial biofilm functionalization through Bap amyloid engineering Leticia Matilla-Cuenca…Jaione Valle. npj Biofilms and Microbiomes 8:62 (2022) MATERIALS Convection Driven Ultrarapid Protein Detection via Nanobody-Functionalized Organic Electrochemical Transistors Anil Koklu…Sahika Inal. Advanced Materials July 2022 MATERIALS Development of a synthetic nanoparticle vaccine presenting the HIV-1 envelope glycoprotein Phindile Ximba…Edward Rybicki. Nanotechnology 26 July 2022 IMMUN Nanocarriers based on bacterial membrane materials for cancer vaccine delivery Xiao Zhao…Guangjun Nie. Nature Protocols (2022) July IMMUN Mechanical regulation of talin through binding and history-dependent unfolding Narayan Dahal…Ionel Popa. Science Advances 15 Jul 2022 MATERIALS TRAIL & EGFR affibody dual-display on a protein nanoparticle synergistically suppresses tumor growth Heejin Jun…Eunhee Kim. J Control Release 2022 Jul 6 MATERIALS Self-Assembling Protein Surfaces for In Situ Capture of Cell-Free-Synthesized Proteins Ella Lucille Thornton…Lynne Regan. Front. Bioeng. Biotechnol., 07 July 2022 MATERIALS Neutralizing monoclonal antibodies elicited by mosaic RBD nanoparticles bind conserved sarbecovirus epitopes Chengcheng Fan…Pamela J. Bjorkman. bioRxiv Jun 2022 IMMUN Steric accessibility of the N-terminus improves the titer and quality of recombinant proteins secreted from Komagataella phaffii Neil C. Dalvie…Christopher Love. bioRxiv Jun 2022 and Microb Cell Fact Sept 2022 IMMUN 650 Mosaic RBD nanoparticles protect against challenge by diverse sarbecoviruses in animal models Alexander A. Cohen…Pamela J. Bjorkman.. Science 5 Jul 2022 IMMUN Liquid–Liquid Phase Separation and Assembly of Silk-like Proteins is Dependent on the Polymer Length Laura Lemetti…A. Sesilja Aranko. Biomacromolecules 2022 Jul MATERIALS METALLOENZYMES FOR BIOMOLECULAR RECOGNITION OF N-TERMINAL MODIFIED PEPTIDES Patent WO/2022/147334 ENCODIA, INC. [US]/[US] ENZ SEQUENCING ANTIBODY-GUIDED PCSK9-MIMICKING IMMUNOGENS LACKING 9-RESIDUE SEQUENCE OVERLAP WITH HUMAN PROTEINS Patent WO/2022/147373 Publication Date 07.07.2022 KWONG, Peter D. et al. IMMUN Sequentially co-immobilized PET and MHET hydrolases via Spy chemistry in calcium phosphate nanocrystals present high-performance PET degradation Kun Chen…Yan Sun. Journal of Hazardous Materials. Jul 2022 MATERIALS SpySwitch enables pH- or heat-responsive capture and release for plug-and-display nanoassembly. Vester SK, Rahikainen R, Khairil Anuar INA, Hills RA, Tan TK, Howarth M. Nat Commun. 2022 Jun 28;13(1):3714. IMMUN A modular dCas9-based recruitment platform for combinatorial epigenome editing Tessa Swain…Ryan Lister. bioRxiv Jul 2022 and Nucleic Acids Research, gkad1108, 24 November 2023 NUCLEIC ACID BINDING PROTEIN FORMULATIONS AND USES THEREOF Patent WO/2022/140795 Publication Date 30.06.2022 SPOTLIGHT THERAPEUTICS [US]/[US] Multienzyme Assembly on Caveolar Membranes In Cellulo Qixin Wei…Jiang Xia. ACS Catal. 2022, 12, 8372–8379 ENZ NUCLEIC ACID VACCINES Patent WO/2022/129547 Publication Date 23.06.2022 UNIVERSITY OF COPENHAGEN [DK]/[DK] ADAPTVAC APS [DK]/[DK] IMMUN UNIVERSAL BACTERIOPHAGE T4 NANOPARTICLE PLATFORM TO DESIGN MULTIPLEX SARS-COV-2 VACCINE CANDIDATES BY CRISPR ENGINEERING Patent WO/2022/130196 Publication Date 23.06.2022 RAO, Venigalla B. ZHU, Jingen IMMUN Freeze-Drying of a Capsid Virus-like Particle-Based Platform Allows Stable Storage of Vaccines at Ambient Temperature Kara-Lee Aves…Adam F. Sander. Pharmaceutics June 2022 IMMUN Two-Dimensional Protein Nanoarray as a Carrier of Sensing Elements for Gold-Based Immunosensing Systems Jingya Tang…Dian-Bing Wang. Anal. Chem. June 21, 2022 MATERIALS Induction of Broadly Cross-Reactive Antibody Responses to SARS-CoV-2 Variants by S1 Nanoparticle Vaccines Cong Sun…Yin-Feng Kang. J Virol Jun 2022 IMMUN Peptide-Inspired One-Step Synthesis of Surface-Functionalized Fe3O4 Magnetic Nanoparticles for Oriented Enzyme Immobilization and Biocatalytic Applications Yanhong Zhou…Guangya Zhang. ACS Appl. Nano Mater. Jun 2022 ENZ DNA passes through cohesin’s hinge as well as its Smc3-kleisin interface James E Collier and Kim A Nasmyth. bioRxiv May 2022 and eLife Sept 2022 Increased Thermal Stability of the Carbonic Anhydrase Enzyme Complex for the Efficient Reduction of CO2 through Cyclization and Polymerization by Peptide Bonding Habin Sun…Jeong Eun Hyeon. Process Biochemistry, 6 June 2022 ENZ A Powassan virus domain III nanoparticle immunogen elicits neutralizing and protective antibodies in mice Ryan J. Malonis…Jonathan R. Lai. PLoS Path Jun 2022 IMMUN ENGINEERED PROBIOTIC COMPOSITIONS AND USES THEREOF Patent WO/2022/119926 Publication Date 09.06.2022 CUBILLOS, Andres Fernando, COLLINS, James J. Quaternary structure independent folding of voltage-gated ion channel pore domain subunits Cristina Arrigoni…Daniel L. Minor Jr. Nat Struct Mol Biol, Jun 2022 RECONSTITUTION OF A SPLIT-HALOTAG VIA ORTHOGONAL TAG-BINDING DOMAINS WO/2022/115696 Publication Date 02.06.2022 HUANG, Bo et al. MULTIPLEXED PROFILING OF RNA AND DNA MODIFICATIONS Patent WO/2022/115608 Publication Date 02.06.2022 ALIDA BIOSCIENCES, INC. [US]/[US] CRISPR-mediated protein-tagging signal amplification systems for efficient transcriptional activation and repression in Saccharomyces cerevisiae Haotian Zhai…Jin Hou. Nuc Acid Res May 2022 LIGHT-CONTROLLED VIRAL TRANSDUCTION Patent WO/2022/106690, Publication Date 27.05.2022 HÖRNER, Maximilian; WEBER, Wilfried Programmable protein topology via SpyCatcher-SpyTag chemistry in one-pot cell-free expression system Qingning Ye…Yuan Lu. Protein Science 21 May 2022 625 A loosened gating mechanism of RIG-I leads to autoimmune disorders Yixuan Lei…Jie Zheng. Nucleic Acid Res May 2022 IMMUN MULTIVALENT CARRIERS AND RELATED VACCINE COMPOSITIONS Patent WO/2022/103891 Publication Date 19.05.2022 COHEN, Alexander, A.; BJORKMAN, Pamela, J. IMMUN SCAFFOLDED ANTIGENS AND ENGINEERED SARS-COV-2 RECEPTOR-BINDING DOMAIN (RBD) POLYPEPTIDES WO/2022/104265 Publication Date 19.05.2022 THE SCRIPPS RESEARCH INSTITUTE [US]/[US] EMMUNE, INC. [US]/[US] IMMUN Non-Covalently Associated Streptavidin Multi-Arm Nanohubs Exhibit Mechanical and Thermal Stability in Protein-Network Materials David S Knoff…Minkyu Kim. Biomacromolecules Sept 2022 and bioRxiv May 2022 MATERIALS A Capsid Virus-Like Particle-Based SARS-CoV-2 Vaccine Induces High Levels of Antibodies and Protects Rhesus Macaques. Ariane Volkmann…Paul Chaplin. Front. Immunol. 05 April 2022 IMMUN SARS-COV-2 RBD CONJUGATED NANOPARTICLE VACCINE WO/2022/088953 Publication Date 05.05.2022 SUN YAT-SEN UNIVERSITY IMMUN A Magnetosome-Based Platform for Flow Biocatalysis Esther Mittmann…Dirk Schüler. ACS Appl. Mater. Interfaces May 2022 MATERIALS Free energy landscape of two-state protein Acylphosphatase with large contact order revealed by force- dependent folding and unfolding dynamics Xuening Ma…Hu Chen. Phys Rev, Jul 2022 and arXiv Mar 2022 MATERIALS A bacteriophage-based, highly efficacious, needle and adjuvant-free, mucosal COVID-19 vaccine Jingen Zhu…Venigalla B. Rao. mBio Jul 2022 and bioRxiv Apr 2022 IMMUN BACTERIAL MICROCOMPARTMENT VIRUS-LIKE PARTICLES Patent WO/2022/086450 Publication Date 28.04.2022 TAN, Yong Quan et al. METHODS AND COMPOSITIONS FOR RAPIDLY REPLACING CARDIAC MYOSIN BINDING PROTEIN-C IN SARCOMERES Patent Application Number 17559865 Publication Date 21.04.2022 Samantha P. Harris MATERIALS Evolutionary balance between foldability and functionality of a glucose transporter Hyun-Kyu Choi…Tae-Young Yoon. Nature Chemical Biology (Apr 2022) MATERIALS Estimation of microtubule-generated forces using a DNA origami nanospring Ali Nick Maleki…Vladimir A. Volkov. bioRxiv Apr 2022 MATERIALS BTB-BCL6 dimers as building blocks for reversible drug-induced protein oligomerization Lena Nitsch…MikołajSłabicki. Cell Reports Methods Apr 2022 An engineered live biotherapeutic for the prevention of antibiotic-induced dysbiosis Andrés Cubillos-Ruiz…James J. Collins. Nature Biomedical Engineering 2022 ENZ POLYPEPTIDE TERMINAL BINDERS AND USES THEREOF WO/2022/072560 Publication Date 07.04.2022 ENCODIA, INC. [US]/[US] SEQUENCING Engineering functional membrane-membrane interface by InterSpy Hossein Moghimianavval…Taekjip Ha. Small 26 May 2022 and bioRxiv 2022 Mar IMAGING Mosaic RBD nanoparticles protect against multiple sarbecovirus challenges in animal models Alexander A. Cohen…Pamela J. Bjorkman. bioRxiv April 2022 IMMUN B12-induced reassembly of split photoreceptor protein enables photoresponsive hydrogels with tunable mechanics Zhongguang Yang…Fei Sun. Science Advances Apr 2022 MATERIALS From 4-arm star proteins to diverse stimuli-responsive molecular networks enabled by orthogonal genetically encoded click chemistries Hong Kiu Francis Fok…Fei Sun. Polymer Chemistry Apr 2022 MATERIALS Effect of SpyTag/SpyCatcher cyclization on stability and refolding of green fluorescent protein Haiyan Song…Hui Luo. Biotechnology Letters Mar 2022 IMAGING POLYPEPTIDE TAGGED NUCLEOTIDES AND USE THEREOF IN NUCLEIC ACID SEQUENCING BY NANOPORE DETECTION Patent Application Number 17201834 Publication Date 24.03.2022 Roche Sequencing Solutions, Inc. SEQUENCING SpyChIP identifies cell type-specific transcription factor occupancy from complex tissues Siqian Feng, Richard S Mann. PNAS June 13, 2022 and bioRxiv Mar 2022 In vitro refolding of the structural protein VP1 of parvovirus B19 produces virus-like particles with functional VP1 unique region. Soto-Valerio, I.A….Bustos-Jaimes, I. Virology (2022) March IMMUN Comparison of Bacterial Expression Systems Based on Potato Virus Y-like Particles for Vaccine Generation by Anete Ogrina…Andris Zeltins. Vaccines 2022, 10(4), 485 IMMUN 301-600 publications here. 1-300 publications here.
It's the oldest question of all, George.  Who can spy on the spies?  ― John le Carré
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